Dynamics of actin polymerisation during the mammalian single-cell wound healing response

Objective

The contribution of actomyosin contractile rings in the wound healing program of somatic cells as never been directly assessed. This contrast with the events characterising the wound healing response of in wounded Xenopus oocytes, in which formation and contraction of an actomyosin ring provides a platform for cytoskeletal repair and drives the restoration of proper plasma membrane composition at the site of injury. As such, we aimed to characterize, using high-resolution live-cell confocal microscopy, the cytoskeletal repair dynamics of HeLa cells.

Results

We confirm here that the F-actin enrichment that characterizes the late repair program of laser-wounded cells is mostly uniform and is not associated with co-enrichment of myosin-II or the formation of concentric zones of RhoA and Cdc42 activity.

Single-cell repair is highly context-dependent. Small, nanometer-sized plasma membrane (PM) wounds are thermodynamically favored to reseal spontaneously [1, 2]. Conversely, the repair of the very large wounds studied in Xenopus laevis and sea urchin oocytes, and in early drosophila embryos, requires the involvement of rapid exocytic events as well as the formation and “explodosis” of a large membrane patch [3]. In the same models, restoration of normal PM composition and of damaged cytoskeletal structures involves the cooperation between the contraction of an actomyosin ring [4] and actin polymerisation [5]. The repair of clinically and physiologically-relevant wounds experienced by somatic cells such as neurons, myofibers, and pneumonocytes also involves exocytic events are followed by the same core steps of wound stabilization: exocytosis of intracellular vesicles, followed by spontaneous repair, or the removal of damaged PM components by shedding or endocytosis (reviewed in [6]). It is also worth noting that none of these processes are able to fully restore a cell’s PM to its pre-wounded state [7], or restore damaged underlying cortex cytoskeletal structures [8, 9]. Somatic cells possess the necessary machinery to form contractile ring (CR) structures [10], but formation and contraction of a CR following PM injury have never been directly observed or confirmed. In Xenopus oocytes, CRs have been observed in response to puncture wounds of 150 µm in diameter [11] and laser wounds of approximately 50–100 µm in diameter [5, 12]. The wounds created in previous assays performed in mammalian cells are usually much smaller, historically in the range of a few μm in diameter. Is the smaller nature of the wounds created in somatic cells the reason for the apparent absence of a CR upon wounding?

We present here the results of a wound healing assay aiming to answer these questions and to directly investigate the presence of an CR following PM injury in somatic cells. Data was acquired by way of a laser-mediated wounding assay which created very large (10 μm range) wounds on the bottom surface of HeLa cells. We then imaged the PM and cytoskeletal repair dynamics using high-resolution, live confocal microscopy. We report strong microscopic evidence confirming that the wound healing response program of mammalian cells is mostly driven via actin polymerisation and does not necessarily involve the presence of a CR.

Materials and methods

Cell culture

HeLa cells purchased from ATCC (ATCC CCL-2) were maintained at 37 °C with 5% CO₂, in MEM, supplemented with 10% FBS, 1% Penicillin–Streptomycin, and 1% Glutamax (all reagents from Gibco, Canada). For live imaging, cells were seeded on glass-bottom dishes (1.5 thickness; MatTek, USA) and imaged in phenol red-free imaging media.

PM labelling and targeted laser ablation of the PM

CellMask Deep Red Plasma Membrane Stain (Invitrogen, USA) was used to label the PM as per the manufacturer’s instructions and was used to target multiple sequential laser pulses of a MicroPoint UV laser (Andor, Northern Ireland) to a ~ 95 µm² (11 µm diameter) area of the PM on the side contacting the dish (i.e. basal side). The success of PM ablation was made on the basis of the creation of a visually detectable gap in the fluorescent signal for both the PM (CellMask) and cytoskeletal compartments. Formation of bona fide PM and cytoskeletal wounds (vs photobleaching) was confirmed via a series of independent wounding/repair. FM1-43 (N-(3-Triethylammoniumpropyl)-4-(4-(Dibutylamino) Styryl) Pyridinium Dibromide; Invitrogen, USA) wounding assays [13] (Additional file 1), and re-assessed at regular intervals.

Transfection of genetically-encoded probes for live imaging

Cytoskeletal dynamics and RhoGTPase activity were tracked using a series of genetically encoded probes: F-actin (calponin homology domain of utrophin; PCS2-UtrCH-GFP; Addgene #26737 or PCS2-UtrCH-mCherry; Addgene #26740), myosin-II (RFP-tagged myosin regulatory light chain 1; PCS2-MRLC-RFP), microtubules (GFP-labelled microtubule binding-domain of ensconsin; PCS2-EMTB-3xGFP; Addgene # 26741), active RhoA (RhoA-binding domain of rhotekin; PCS2- γGBD-GFP; Addgene #26732), and Cdc42 (Cdc42 binding domain of N-WASP; PCS2- ωGBD-GFP Addgene # 26734). All plasmids were transfected using PolyJet In Vitro DNA transfection reagent (SignaGen, USA), as per the manufacturer’s instructions.

Imaging

Imaging was performed at the Cell Imaging and Analysis Network (CIAN) imaging facility at McGill University, using a Quorum WaveFX-X1 spinning disk confocal system on a Leica DMI6000B inverted microscope, two Hamamatsu “ImagEM X2 C9100-23B” EM-CCD cameras, and an 63×/1.40 oil objective (lBL, HCX PL APO; 506192). Cells were kept at 37 °C with 5% CO₂ using a Live Cell Instruments Chamlide TC environmental control system. Acquisition was performed using MetaMorph Microscopy Automation and Image Analysis Software (Molecular Devices LLC, USA) and consisted of Z-stacks (11 slices, 0.2 µm apart), taken every 10 s for at least 15 min. 1 × 1 binning, 1 gain, and 100 EM gain was used throughout.

Images were further processed using ImageJ software (National Institutes of Health, USA). Quantitation of fluorescence at the wound site, was performed on maximum intensity projections (3 slices, 0.2 µm apart). Average fluorescence intensity was measured at every 10-s time point, at four 95 μm² sites: the wound site region (FI_W) and three control cytoplasmic regions within the cell (FI_C). The relative fluorescence intensity at the wound site (FI_R) was calculated by dividing FI_W by the average of the three FI_C. To enable cell-to-cell comparisons, the resulting FI_R were then further normalized to the average FI_R on the same unwounded cell. Micrographs, kymographs, and orthogonal views were prepared from maximum intensity projections (MIPs) of the entire imaged volume (10 slices, 0.2 µm apart).

Statistics

Statistical analyses were performed using GraphPad Prism 7.04 (GraphPad Software Inc., USA) and consisted of repeated measure 2-way ANOVAs, followed by Dunnett’s multiple comparisons tests. Significance was set at p < 0.005.

Results and discussion

We provide here substantial evidence that under normal 2D-culture conditions, mammalian cytoskeletal repair or large PM and cytoskeletal injuries involves actin polymerisation, without the formation of actomyosin contractile arrays.

Wound healing response involves uniform actin polymerisation, but not myosin-II accumulation

We first set out to also track the spatio-temporal dynamics of actin polymerisation that follows laser-mediated injury of HeLa cells. As expected, the ablated area showed clear indications of actin polymerisation following wounding (Figs. 1 and 3a; Additional file 2, Additional file 3, Additional file 4, Additional file 5, Additional file 6, Additional file 7, Additional file 8 and Additional file 9). Furthermore, UtrCH FI_R rapidly rose above the baseline post-wounded levels (significant after 30 s), which was significantly higher than the pre-wounding baseline levels after 6 min (Fig. 1b), suggesting enrichment of F-actin at the wound site. CR-mediated repair involves the formation of two functionally and structurally distinct zones of actin polymerisation: a dense ring of F-actin and Myosin-II located at the wound edge, which is surrounded by a wider array of perpendicularly oriented F-actin [5, 11, 14, 15]. Neither F-actin patterns were readily observable (Fig. 1a, 3a) during the course of more than 37 individual wounding assays. Kymographic and line scan analyses (Fig. 1c, 3b, c; Additional file 2) confirmed this qualitative assessment and also failed to reveal any other significant spatially defined enrichment of the UtrCH signal. Instead, UtrCH signal increased uniformly over the entire area of the wound site, with no noticeable enrichment at the wound edge (Fig. 1c, 3c; Additional file 2). Orthogonal views of the wound site were similarly unable to detect any localized UtrCH signal indicative of a clearly defined actomyosin structure (Fig. 1d).

F-actin is enriched at the wound site following laser-mediated ablation of the PM. a Maximum intensity projections of selected micrographs of the CellMask and UtrCH signals following laser-mediated ablation of the PM of HeLa cells. b Normalized UtrCH fluorescent signal intensity (UtrCH FI_R) at the wound site relative to control regions. Mean and SEM shown of n = 37 cells. c Kymograph of the UtrCH signal before and after laser-mediated ablation of the PM. Cell and targeted area is the same as in the one shown in a. d Resliced cortical volume (each 10 μm) of selected time-points of the wounding assay shown in a, c. Targeted area (95 μm²) is represented by the red circle found on the pre-wounded, PM micrograph. The line scan region used for the creation of the kymograph and resliced volumes correspond to the cyan line (22 μm) displayed on the 0 s, UtrCH micrograph. The cell shown in a, c, and d is representative of n = 37 wounding assays, all of which were used to prepare b. Scale bars = 10 μm

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The spatio-temporal dynamics of myosin-II following injury was also investigated (Fig. 2a), as it is responsible for the actomyosin array’s contractility [11]. We found that the return of myosin-II at the wound site did not follow the same pattern as we observed for F-actin, as the rate of MRLC FI_RW signal accumulation to the wound site (Fig. 2b) was much slower than the one observed for UtrCH, and never went above pre-wounded baseline levels within the time allotted (Fig. 2b). Like F-actin, myosin-II did not show sign of accumulating according to any discernible patterns (Fig. 2a, c and d; Additional file 10). The sequence timing of F-actin and of myosin-II accumulation, broadly confirms the findings of other reports for wounds created on both the apical or basal side of cells, using a variety of wounding methods [16,17,18]. Specific differences in the actin polymerisation dynamics between our and all of the other previous studies could be attributable to the differences in wound size, wounding methods (laser vs mechanical), location (apical vs basal), cell shape and in local tension between the different wounding assays.

Mammalian single-cell repair does not involve myosin enrichment at the wound borders. a Maximum intensity projections of selected micrographs of the CellMask and MRLC signals following laser-mediated ablation of the PM of HeLa cells. b Normalized MLRC fluorescent signal intensity (MRLC FI_R) at the wound site relative to control regions. Mean and SEM shown of n = 12 cells. c Kymograph of the MRLC signal before and after laser-mediated ablation of the PM. Cell and targeted area is the same as in the one shown in a. d Resliced cortical volume (each 10 μm) of selected time-points of the wounding assay shown in a, c. Targeted area (95 μm²) is represented by the red circle found on the pre-wounded, PM micrograph. The line scan region used for the creation of the kymograph and resliced volumes correspond to the cyan line (22 μm) displayed on the 0 s, MRLC micrograph. The cell shown in a, c, and d is representative of n = 12 wounding assays, all of which were used to prepare b. Scale bars = 10 μm

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The mammalian wound healing response does not involve spatially regulated RhoA or Cdc42 signalling, microtubule arrays, or coordination of PM restoration and cytoskeletal repair

The presence of concentric rings of RhoA and Cdc42 activity around the wound edge is a hallmark event of the formation of actomyosin contractile arrays [19]. As such, we set out to characterize the spatio-temporal distribution of RhoA and Cdc42 signalling in the context of our wound healing assay. Neither γGBD (Fig. 3) or ωGBD (Additional file 3) signals coalesced into the clearly defined RhoA or Cdc42 “activity zones” around the wound edge that would be necessary to the formation and contraction of CR, as demonstrated in wounded Xenopus oocytes [19, 20]. Instead, RhoA activity exhibited a pattern that closely matched that of UtrCH and accumulated over the entire area of the wound site (Fig. 3a, c). γGBD signal quickly rose after wounding, but never rose above its “pre-wounded” baseline levels (Fig. 3b). We were unable to observe any quantifiable or observable change in ωGBD following wounding anywhere in the cell, following the wounding event (Additional file 3; Additional file 7). These findings are consistent with a previous study that examined RhoA activity in mouse myoblasts wounded with a single laser blast to the basal side of their PM, which found local RhoA activation signal at the wound site that also failed to show ring-like patterning [18]. The same study attributed this pattern of RhoA activity to the increased reactive oxygen species (ROS) production caused by the increased mitochondrial uptake of calcium through the mitochondrial calcium uniporter (MCU) [18]. Whether mitochondria mediated ROS production also plays a role in the RhoA activation and actin polymerisation events observed in wounds as large as the ones produced within the context of our study remains to be confirmed.

RhoA activity is increased the over entire area of the wound-site. a Maximum intensity projections of selected micrographs of the CellMask, UtrCH, and γGBD signals following laser-mediated ablation of the PM of HeLa cells. b Normalized γGBD fluorescent signal intensity (γGBD FI_R) at the wound site relative to control regions. Mean and SEM shown of n = 12 cells. c Kymographs of the UtrCH and γGBD signals before and after laser-mediated ablation of the PM. Cell and targeted area is the same as in the one shown in a. Targeted area (95 μm²) is represented by the red circle found on the pre-wounded, PM micrograph. The line scan region used for the creation of the kymograph correspond to the cyan line (22 μm) displayed on the 0 s, γGBD micrograph. The cell shown in a, c is representative of n = 12 wounding assays, all of which were used to prepare b. Scale bars = 10 μm

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We also failed to observe the formation of a MT array following injury. Instead, MTs seemed to polymerize from the wound-edge inward (Additional file  6; Additional file 11). This would be consistent with the absence of an actomyosin ring as CR formation is further driven by cortical flow and feedback with microtubules (MTs), which accumulate in a radial array around the wound site and the CR in the Xenopus oocyte model [5, 11, 12]. These observations are also compatible with a study of mouse myoblasts wounded with a single laser point, where MTs were also found to be neither enriched nor necessary for repair [18] and with observations about the dynamics of MTs of mechanically wounded fibroblasts [21].

In echinoderm oocytes, Xenopus oocytes, and early Drosophila embryos, PM and cytoskeletal repair are deeply interconnected (reviewed [6]). The contraction of the actomyosin array not only provides a platform for F-actin polymerisation, but also regenerates normal PM composition as it drags PM inwards through the contracting actomyosin array [14]. We did not observe such coordination in the healing rates of the PM and F-actin wounds (Additional file 12), which again suggests that cells from our wounding assay did not heal according to the classical model of actomyosin array-mediated contraction. It should be noted however, that our assays prioritized the visualization of cytoskeletal events, and as such did not set out to verify the presence of a de facto membrane patch as observable in the Xenopus model or of any of the exocytosis or “explocytosis” events that have been shown to be required for its formation [3].

Conclusions

In mammalian somatic cells, repair of cytoskeletal wounds up to 10 μm is not strictly dependent on the assembly and contraction of an actomyosin array.

Apical and basal side of cells cultured in 2D probably exhibit differences in the properties of their cortex cytoskeleton structures or in their local tension. Exclusive targeting of the basal surface may have biased our results and limited compatibility with previously published mechanically wounded-cells or of the repair processes related to the apical surface.

Raw images used to produce the data are available from the corresponding author on reasonable request.

CR:

contractile ring

Cdc42:

cell division control protein 42 homolog

Drosophila :

Drosophila melanogaster

F-actin:

filamentous actin

PM:

plasma membrane

MT:

microtubule

MRLC:

myosin regulatory light chain

N-WASP:

neural Wiskott-Aldrich syndrome protein

RhoA:

Ras homolog family member A

Rho GTPase:

Rho family of GTP hydrolases

Xenopus :

Xenopus laevis

We thank David Zhou and Quentin Basiren for their critical revision of the manuscript.

Funding

Natural Sciences and Engineering Research Council of Canada (NSERC) Grant Number: 231925.

Affiliations

1. Department of Anatomy and Cell Biology, Faculty of Medicine, McGill University, 3640 University Street, Strathcona Anatomy and Dentistry Bldg, Montreal, QC, H3A 0C7, Canada
   • Corina DeKraker
   • , Laurence Goldin-Blais
   • , Eric Boucher
   •  & Craig A. Mandato

Contributions

This study was primarily designed by CAM and EB, with input from CD. Data acquisition was performed by CD, assisted by LGB. Analysis was performed by CD, assisted by LGB and EB. The manuscript was prepared by CD and EB, with input from LGB and CAM. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Craig A. Mandato.

Ethics approval and consent to participate

Not applicable.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

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