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The draft genome of Labeo catla



Labeo catla (catla), one of the three Indian major carps, is native to the Indo-Gangetic riverine system of India as well as the rivers of Pakistan, Bangladesh, Nepal and Myanmar. Its higher growth rate and compatibility with other major carps, specific surface feeding habit, and consumer preference have increased its popularity in carp polyculture systems among the fish farmers in Indian subcontinent. Recent advancement in sequencing technology coupled with massive parallel sequencing platforms has facilitated accelerated genetic improvement in aquaculture species through integration of genomics tools. A draft genome and allied resources are lacking in catla. Therefore, in the present study, we have performed de-novo assembly of Labeo catla for the first time.

Data description

A male farm reared catla was used for extracting high molecular weight genomic DNA followed by sequencing in Oxford Nanopore and Illumina platforms. Approximately, 80× coverage of sequence data was assembled adopting the hybrid assembly strategy. The assembled genome size of catla was 1.01 Gb containing 5345 scaffolds with N50 value 0.7 Mb and more than 92% BUSCO completeness. Gene annotation resulted in 25,812 predicted genes.


Aquaculture is the rapidly emerging food production sector all over the world and it is going to be the primary source of fish and shellfish for human diet in the coming future [1]. Genetic improvement of performance traits has huge potential to meet the increasing demand of quality animal protein in the event of exponential growth of human population. Well-designed breeding programmes integrated with genomics tools can accelerate the production and productivity. Recent advancement in sequencing technology coupled with massive parallel sequencing platforms has paved the way for expediting genetic improvement programs in aquaculture species.

Labeo catla (catla), one of the Indian major carps, is native to the Indo-Gangetic riverine system of India as well as the rivers of Pakistan, Bangladesh, Nepal and Myanmar. Its higher growth rate and compatibility with other major carps, specific surface feeding habit, and consumer preference have increased its popularity in carp polyculture systems among the fish farmers in India, Bangladesh, Myanmar, Laos, Pakistan and Thailand [2]. L. catla currently accounts for  3.4% of total freshwater aquaculture production worldwide [3]. With an aim to generate consolidated genomics resource for supporting genetic improvement, we have undertaken de-novo assembly of catla for the first time. The draft genome will also be an important resource for comparative genomics, biological and evolutionary studies of cyprinid species.

Data description

One farm-reared mature (2 years old) male catla weighing approximately 1.7 kg was collected from ICAR-Central Institute of Freshwater Aquaculture (CIFA) farm for this study. Before tissue sampling, fish was anesthetized with MS-222 (300 mg/l) and then weighed. High molecular weight genomic DNA was isolated from testis tissue using standard phenol–chloroform method [4]. The qualitative and quantitative assessment of DNA were performed by NanoDrop spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, U.S.A.) and Qubit fluorometer (Invitrogen, Carlsbad, CA, USA) followed by checking on 0.8% agarose gel. Genomic DNA was sheared using a Covaris S2 sonicator (Covaris, Woburn, Massachusetts, USA) to generate fragments in the range of 200 bp to 20 kb. Four Paired end libraries (insert size: 350 bp) for Illumina Nextseq500 platform and one library (mixed insert size) for Oxford Nanopore were prepared and sequenced as per manufacturer’s instruction. A total of 80.28 Gb sequence data (Table 1, Data file 1) [5] were generated after quality check by FastQC tool [6]. The de novo hybrid assembly was performed with default parameters using MaSuRCA 3.2.8 [7] followed by scaffolding and Gap closing with SSPACE v3.0 [8] and GapCloser v1.12b [9], respectively. This yielded 5,345 scaffolds with N50 value of 0.7 Mb (Table 1, Data file 2) [10] and largest fragment of 6.8 Mb. The assembled genome size of catla is 1.01 Gb (Table 1, Data file 3) [11] against an in silico estimated genome size of 0.95 Gb. The evaluation of genome by Benchmarking Universal Single-Copy Orthologs (BUSCO) version 3.0 [12] and using Actinopterygii odb9 core gene set revealed 92% complete, 87.9% complete and single copy, 4.1% complete and duplicated, 4.1% fragmented and 4.05% missing BUSCOs. RepeatModeler [13] was used for de novo repeat modelling which showed 47.58% of repeat content in catla genome. The genome wide simple sequence repeats of assembled catla genome was 391,331.

The catla genome is predicted to contain 25,812 protein-coding genes. Additionally, scaffold_2219 of a size of 16,600 bp, was found to be of mitochondrial origin, with 13 mRNAs, 22 tRNAs and 2 rRNAs. Functional annotation of the final set of predicted protein sequences was carried out by BLAST2GO v5.0. Out of 25,812 genes, 17,500 were found to have GO term assigned to them. The number of protein coding genes identified in catla (25,812) is comparable to the genomes of sequenced diploid cyprinids such as Labeo rohita [14], Ctenopharyngodon idellus [15], Danio rerio [16] and Anabarilius grahami [17]. Orthologous relationship among these species using OrthoVenn [18] showed a total of 8,494 orthologous gene clusters to be shared by all five species, with 1,357 species specific gene clusters. The whole genome sequence data has been deposited in the GenBank (Table 1, Data file 4) [19

Table 1 Overview of data files/data sets


The assembled genome size of Labeo catla is 1.01 Gb constituting 5345 scaffolds. The number of unassembled regions is 649 and the number of bases positioned in this gap is 0.8 Mb.

Availability of data and materials

This Whole Genome project has been deposited at DDBJ/ENA/GenBank under the Bioproject id: PRJNA557138 and Acc no: VONZ00000000 ( [19]. The version described in this paper is version VONZ01000000. Please see Table 1 and references [5, 10, 11, 19] for details and links to the data.



Food and Agriculture Organisation of the United Nations


Giga base pair


Maryland Super-Read Celera Assembler


Benchmarking Universal Single-copy Orthologs


Gene ontology


Mega base pair


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We acknowledge the financial support by the Department of Biotechnology, Govt. of India, New Delhi under Centre of Excellence program. Authors are thankful to the Director, ICAR-CIFA, Bhubaneswar for providing the facilities to work under the project. We thank Dr. P. C. Das, ICAR-CIFA, Bhubaneswar for providing the farm reared catla sample.


This work was supported by the Department of Biotechnology, Govt. of India under the Grant No. BT/AAQ/3/SP13797/2017.

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Authors and Affiliations



PD, JKS, KDM and PKM conceptualised and designed the project. PD and LS designed the experiments. BS, GD, LS, PD and UKU performed sample collection, tissue extraction and DNA isolation. LS, BS and GD performed data analysis. PD, LS and BS and GD wrote and reviewed the manuscript. All the authors read and approved the manuscript.

Corresponding author

Correspondence to Paramananda Das.

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All handling of fish was carried out following the guidelines for control and supervision of experiments on animals by the Government of India and approved by Institutional Animal Ethics Committee (AEC) of ICAR-CIFA.

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The authors declare that they have no competing interests.

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Sahoo, L., Das, P., Sahoo, B. et al. The draft genome of Labeo catla. BMC Res Notes 13, 411 (2020).

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