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Table 1 Origins and termini count under slow and fast growth

From: Chromosome segregation in B. subtilis is highly heterogeneous

  Slow growth (25 °C) Fast growth (37 °C)
  Foci/cell nt Foci/cell nt
PG26 (origin tag) 1.8 ± 0.51 620 4.42 ± 1.49 848
AT62 (terminus tag) 1.49 ± 0.6 700 1.89 ± 0.66 848
PG26 Control 25 °C 1 h
37 °C
1.5 h
37 °C
2 h
37 °C
2.5 h
37 °C
3 h
37 °C
Percent polyploidy and average number of origins over time
 OD 0.7 0.11 0.3 0.7 1.8 2.5
 % monoploida 37.1 11 9 11 10 13
 % diploidb 59.2 70 71 70 68 68
 % polyploidc 3.7 19 20 19 22 19
 Origins per cell 1.8 ± 0.51 4.8 ± 1.6 4.9 ± 2 4.9 ± 1.1 4.7 ± 0.55 4.1 ± 0.89
 nt 620 300 300 350 400 400
AT62 Control
25 °C
1 h
37 °C
1.5 h
37 °C
2 h
37 °C
2.5 h
37 °C
3 h
37 °C
Percent ploididity and average number of origins over time
 OD 0.7 0.13 0.4 1 1.9 3
 % monoploidd 35 17 13 12 15 12
 % diploide 61 74 72 71 69 75
 % polyploidf 4 9 15 17 16 13
 Termini per cell 1.49 ± 0.6 1.7 ± 0.5 1.93 ± 0.6 1.8 ± 1.1 1.72 ± 0.73 1.95 ± 0.8
 nt 700 200 300 300 400 400
AT62 Control
25 °C
1.5 h
37 °C
2 h
37 °C
2.5 h
37 °C
Percent ploididity from automatically calculated average DAPI fluorescence
 OD 0.7 0.4 1 1.9
 % monoploid 27.8 11.7 15 11.5
 % diploid 67.5 68.3 63.3 62.25
 % polyploid 4.6 20 21.7 26.25
 nt 540 300 300 400
  1. % polyploidy calculated over all time points at 37 °C = 23.65%.
  2. *nt refers to the total number of cells counted in each experiment
  3. a1 or 2 origins
  4. b2 to 4 origins
  5. c4 to 8 origins
  6. *nt refers to the total number of cells counted in each time interval.
  7. d1 terminus
  8. e2 termini
  9. f> 2 termini