Skip to main content

Chromosome segregation in B. subtilis is highly heterogeneous

Abstract

Objective

The bacterial cell cycle comprises initiation of replication and ensuing elongation, concomitant chromosome segregation (in some organisms with a delay termed cohesion), and finally cell division. By quantifying the number of origin and terminus regions in exponentially growing Bacillus subtilis cells, and after induction of DNA damage, we aimed at determining cell cycle parameters at different growth rates at a single cell level.

Results

B. subtilis cells are mostly mero-oligoploid during fast growth and diploid during slow growth. However, we found that the number of replication origins and of termini is highly heterogeneous within the cell population at two different growth rates, and that even at slow growth, a majority of cells attempts to maintain more than a single chromosome at all times of the cell cycle. Heterogeneity of chromosome copy numbers may reflect different subpopulations having diverging growth rates even during exponential growth conditions. Cells continued to initiate replication and segregate chromosomes after induction of DNA damage, as judged by an increase in origin numbers per cell, showing that replication and segregation are relatively robust against cell cycle perturbation.

Introduction

The segregation of chromosomes is a central part of the cell cycle in all kinds of cells, yet poorly understood in bacteria [1, 2]. In a previous study, cell cycle parameters of Bacillus subtilis have been characterized by determination of population averages of DNA content and nucleoid morphology at four different growth rates. The pattern of increased numbers of replication forks at higher growth rates was similar compared to Escherichia coli cells: at low growth rates, B. subtilis was mostly mono or diploid (later in the cell cycle), meaning cells had one chromosome copy or two, while cells became mero-oligoploid at higher growth rates [3], meaning they contain several origin regions per chromosome copy because of overlapping round of replication. More recent publications have shown that the situation is different from what was calculated, namely that few cells are monoploid, even in poor growth medium [4], and that during exponential phase, most cells are mero-oligoploid in rich medium, and reduced chromosome copy numbers during entry into stationary phase [5].

In our recent study, we have shown that the pattern of chromosome segregation follows that of a linear, directed manner, and could be explained by entropy driven segregation based on diffusion [6]. In this work, we extend these analyses by scoring the number of origin or terminus regions, visualized by FROS, at two different growth rates, namely minimal and rich medium. We also characterized chromosome copy numbers by quantitative DAPI staining of the DNA, and investigated changes in the number of origins and termini after induction of DNA damage via Mitomycin C.

Main text

Polyploidy of Bacillus subtilis

We wished to determine the ploidy status of cells growing at a defined rate, and counted a) number of origins, b) of termini (stained by FROS), and c) number of chromosomes by quantitative DNA staining (DAPI intensity). We also used nucleoid morphology as a determinant for the number of chromosomes per cell, in combination with origin and terminus numbers. We used all four parameters because counting of FROS signals leads to an underestimation of copy numbers, since duplicated regions that are separated less than 250 nm will appear as one signal. All strains are described in [6] and were characterized in this prior work.

The numbers of origins and termini (see Fig. 1 for examples of imaging) were counted through visual scoring of signals per cell under conditions of slow growth in S750 minimal medium (no amino acids) at 25 °C, with a doubling time of 90 min, or during fast growth at 37 °C in LB rich medium (doubling times of 26 min, similar to cells lacking a FROS system) at an OD600 ranging between 0.5 and 3, and the results are summarized in Table 1. As chromosomes can have multiple replication forks, and thus termini, we used the number of termini (and DAPI staining, see below) to determine the ploidity status of cells. The termini counts show that Bacillus subtilis is mostly diploid irrespective of the growth conditions, but that it is mero-polyploid in rich medium, where it can have multiple (up to 8) origins per cell (Table 1, first panel). The chromosome exists as a distinct, compact structure termed the ‘nucleoid’, for the entire cell cycle. Near the end of DNA replication, the nucleoid adopts a bi-lobed, dumb-bell shape that ultimately splits into two independent structures prior to cytokinesis [7]. In the terminus-tag strain, chromosomes forming a dumb-bell shape were counted as two nucleoids when they corresponded with two foci for the terminus tag. However, when only one focus was detected in the GFP (terminus tag) channel, a dumb-bell was considered as two nuclei when each lobe measured > 0.55 µm, or when the lobes were separated by at least 3 dark pixels. The offset of 0.55 was chosen through measuring several small separated lobes and averaging them. For the origin tag strain, the number of nucleoids was used for the classification of cells. Nucleoids with a dumb-bell shape were considered as one chromosome when the lobes were smaller than 0.7 µm and not separated by a minimum of 3 dark pixels. Cells with three or more distinct nucleoids were considered polyploid (Fig. 1), see also Fig. 1a in [6]. Elongated nucleoids occupying > 2.5 µm of the cell length were counted as 2 distinct chromosomes. The numbers of origins and termini were also counted every half an hour in in LB at 37 °C, starting from an OD600 of 0.1. Results are summarized in Table 1, second and third panels.

Fig. 1
figure1

Exponentially growing cells of strain AT62 (terminus tag). a Example are shown of monoploid (dark blue rectangle), diploid (light blue rectangle), and polyploid cells (White rectangle). Green dashes point at the termini, the yellow dash represents 0.55 µm (the minimal length of a single nucleoid), and the red dash represents a distance of at least 3 pixels between 2 nuclei. b Cells grown in minimal medium at 25 °C: Cells are either diploid or monoploid. c Cells grown in LB at 37 °C. An elongated cell with 4 nucleoids is shown. White bars represent 2 µm

Table 1 Origins and termini count under slow and fast growth

Interestingly, the number of termini did not increase strongly at fast growth compared to slow growth, while the number of origins increased with growth rate, consistent with the observation that B. subtilis is diploid most of the time. However, the number of termini could be underestimated because in many cases it was difficult to tell if the focus between 2 nuclei corresponded to one or two termini. Based on the counted number of nuclei according to the above criteria, there was always a subset of polyploid cells under fast growth, but this subset never exceeded 22% for the origin tag strain (PG26), and 18% for the terminus tag (AT62) strain (Table 1). The total polyploidy calculated over all time points under fast growth conditions for both strains was 17.2% and 15.06%, respectively (Table 1, second and third panels). Fig. S1 (https://doi.org/10.6084/m9.figshare.12792407.v1) shows examples of monoploid, diploid, and polyploid cells. Most strikingly, under slow and even rapid growth conditions, fractions of monoploid, diploid and polyploidy cells were present, revealing a much higher heterogeneity of chromosome contents than previously anticipated.

Estimation of the number of nuclei based on DAPI fluorescence

Although manual counting of terminus signals provided a good estimate on the polyploidy state of B. subtilis, it was prone to subjectivity and hence needed to be corroborated with an automated count. For this purpose, the ChainTracer software (Norbert Vischer, University of Amsterdam) was used together with the ImageJ plugin ObjectJ [8] to automatically calculate the amount of DAPI fluorescence in the cell.

The software can detect cell chains in a hyperstack, and extracts single cells from chains by detecting the septa and cell borders from channel containing the membrane- stain images. Integrated density of fluorescence (sum all of the pixels within a region) from the DAPI channel of the hyperstack is calculated, which quantifies the amount of DNA present in the cell. The terminus tag strain was chosen to perform the count under slow and fast growth. A threshold was specified for monoploid cells in each condition by averaging through fluorescence intensity of at least 30 cells measuring between 1.1 µm and 2 µm (i.e. the smallest cells) in each condition.

Average intensity of nucleoids in small cells was comparable in all counted samples. Monoploid cells were taken to have 1–1.69 genomes calculated from the threshold, diploid cells between 1.7 and 2.69, and polyploid cells to contain ≥ 2.69 genomes. Results are shown in Table 1, fourth panel. The percent polyploidy was underestimated by the manual count when compared to the automated based count. However, from both cases, it can be clearly deduced that B. subtilis is preferably diploid under slow and fast growth conditions, and that the number of polyploid cells is very small under slow growth and increases with the growth rate under fast growth. Cell length in minimal medium ranged between 1.894 µm and 4.112 µm, with an average of 3.05 ± 0.47 µm (n = 252). At faster growth both strains showed a direct correlation between the chromosomal content and the cell length whereby the polyploid cells were considerably longer than the diploid and the monoploid cells.

Thus, a B. subtilis culture contains cells with strongly diverging numbers of chromosomes and origin regions. This has to be taken into account when calculating cell cycle parameters from doubling times and replication periods. Interestingly, it has recently been shown that small RNAs induce diversity in transcription repressor AbrB levels generating heterogeneity in growth rates during the exponential growth phase. This leads to subpopulations of fast- and slow-growing B. subtilis cells, which has been proposed to increase fitness of the population when it has to deal with changes between favourable and unfavourable conditions [9].

Effect of DNA double strand breaks on the polyploidy state of B. subtilis

In order to assess the effects of DNA double strand breaks on the chromosome count as well as the number of origins in B. subtilis, Mitomycin C (MMC) was added to a final concentration of 50 ng/ml to cells growing in minimal medium at 25 °C. In B. subtilis, MMC addition leads to a RecA-dependent transcriptional (SOS) response, and also to a RecA-independent response, which is mediated in part by initiator of replication protein DnaA [10]. MMC was shown to cause a relative increase of the dosage of genes proximal to the origin of replication relative to genes in the terminus region [10], which might be caused by re-initiation of replication in the absence of cell division, or in other words, because chromosome segregation and replication continue while cell division is blocked.

We wanted to analyse the number of origins as well of termini in cells treated with MMC. Addition of MMC resulted in a strong reduction of growth for three hours. Cells imaged 30 min after MMC addition showed nucleoids with a condensed morphology (Fig. 2), and a majority of cells (~ 70% of 250 cells counted) contained one nucleoid instead of two, and 2.5 origins on average. The remaining cells had two nucleoids (Fig. 2). 90 min after MMC addition, around 15% of the cells were dead in each imaging field (as judged from high fluorescence throughout the cells), and were excluded from the analyses. The cells were elongated, average cell length increased from 2.87 ± 0.52 µm during exponential growth (n = 150 cells) to 4.4 ± 1.4 µm 90 min after MMC addition. Cell length fell in a range between 1.6 and 7.7 µm, with 20% of the cells being longer than 5 µm, revealing high heterogeneity. The number of origins increased from around 2 per cell prior to the induction of damage to 3.5 ± 1.4 (n = 200 cells). Of note, 16% of the MMC-treated cells had 5 origins or more, with a few having up to 8 origins, and a subset of the cells was anucleate (around 3%). 80% of cells longer than 5 µm contained an elongated, unsegregated nucleoid, and 0.5% of the cells had nucleoids bisected by a septum, evidence of chromosome segregation defects. The terminus tag strain showed comparable phenotypes, in that the cells were elongated and nucleoids were decondensed, 90 min after MMC addition. Excluding the dead cells, the average number of termini was 1.7 ± 0.6 (n = 224 cells), with 7.14% of the cells having 3 termini or even 4 in very rare cases.

Fig. 2
figure2

Cells of strain AT26 (origin tag) after DNA damage induction. a 30 min after addition of MMC, b, c 90 min after MMC addition. In a an example of one condensed nucleoid (white box) and fused nucleoids (red box) are shown. b An example of decondensed DNA in an elongated cell (green box), and c DNA bisected by a septum (green box). The red arrow points to the bisected nucleoid, while the white arrow shows the septum in question. Green lines point at the origins, of which up to seven can be seen in one cell in b White bars indicate 2 µm

These findings show that although MMC induces events of replication arrests due to interstrand crosslinks, and leads to atypical nucleoid morphology, most MMC-treated cells were still able to complete a replication cycle, or even reinitiated another cycle, giving rise to a double set of chromosomes after induction of DNA damage. The findings are in accordance with the report that MMC slows down the cell cycle considerably but does not stop it completely [6], and with findings from Goranov et al. showing that double strand breaks induced by MMC slow down the replication elongation beyond the origin proximal region [10].

Limitations

As stated in reference [6], a small fraction of cells containing the FROS system showed irregularities in segregation. Cells were taken from exponential growth conditions and were imaged within 10 min, minimally influencing the actual number of origins and termini. Due to the resolution of light, origin or terminus regions separated by less than 250 nm would appear as a single signal, leading to underestimation of actual numbers.

Availability of data and materials

All data generated or analysed during this study are included in this published article. Raw images are available upon request at the corresponding author.

Abbreviations

FROS:

Fluorescent operator/repressor system

DAPI:

4′,6-Diamidin-2-phenylindol

References

  1. 1.

    Graumann PL. Chromosome architecture and segregation in prokaryotic cells. J Mol Microbiol Biotechnol. 2014;24(5–6):291–300.

    CAS  Article  Google Scholar 

  2. 2.

    Badrinarayanan A, Le TB, Laub MT. Bacterial chromosome organization and segregation. Annu Rev Cell Dev Biol. 2015;31:171–99.

    CAS  Article  Google Scholar 

  3. 3.

    Sharpe ME, Hauser PM, Sharpe RG, Errington J. Bacillus subtilis cell cycle as studied by fluorescence microscopy: constancy of cell length at initiation of DNA replication and evidence for active nucleoid partitioning. J Bacteriol. 1998;180:547–55.

    CAS  Article  Google Scholar 

  4. 4.

    Wang X, Montero Llopis P, Rudner DZ. Bacillus subtilis chromosome organization oscillates between two distinct patterns. Proc Natl Acad Sci U S A. 2014;111(35):12877–82.

    CAS  Article  Google Scholar 

  5. 5.

    Bottinger B, Semmler F, Zerulla K, Ludt K, Soppa J. Regulated ploidy of Bacillus subtilis and three new isolates of Bacillus and Paenibacillus. FEMS Microbiol Lett. 2018;365:4.

    Article  Google Scholar 

  6. 6.

    Najjar N, Geisel D, Schmidt F, Dersch S, Mayer B, Hartmann R, Eckhardt B, Lenz P, Graumann PL. Chromosome segregation in Bacillus subtilis follows an overall pattern of linear movement and is highly robust against cell cycle perturbations. Sphere. 2020;5:3. https://doi.org/10.1128/mSphere.00255-20.

    Article  Google Scholar 

  7. 7.

    Woldringh CL, Nanninga N. Structural and physical aspects of bacterial chromosome segregation. J Struct Biol. 2006;156(2):273–83.

    CAS  Article  Google Scholar 

  8. 8.

    Pandey R, Ter Beek A, Vischer NO, Smelt JP, Brul S, Manders EM. Live cell imaging of germination and outgrowth of individual Bacillus subtilis spores; the effect of heat stress quantitatively analyzed with SporeTracker. PLoS ONE. 2013;8(3):e58972.

    CAS  Article  Google Scholar 

  9. 9.

    Mars RA, Nicolas P, Ciccolini M, Reilman E, Reder A, Schaffer M, Mader U, Volker U, van Dijl JM, Denham EL. Small regulatory RNA-induced growth rate heterogeneity of Bacillus subtilis. PLoS Genet. 2015;11(3):e1005046.

    Article  Google Scholar 

  10. 10.

    Goranov AI, Kuester-Schoeck E, Wang JD, Grossman AD. Characterization of the global transcriptional responses to different types of DNA damage and disruption of replication in Bacillus subtilis. J Bacteriol. 2006;188(15):5595–605.

    CAS  Article  Google Scholar 

Download references

Acknowledgements

Not applicable

Funding

Open Access funding enabled and organized by Projekt DEAL. This work was supported by the University of Marburg. We also acknowledge funding by the Katholischer Akademischer Ausländer-Dienst and the BMBF funded graduate school NANOKAT.

Author information

Affiliations

Authors

Contributions

NEN performed all experiments, analysed the data and co-wrote the manuscript. PLG conceived of the study and co-wrote the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Peter L. Graumann.

Ethics declarations

Ethics approval and consent to participate

Not applicable.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

El Najjar, N., Graumann, P.L. Chromosome segregation in B. subtilis is highly heterogeneous. BMC Res Notes 13, 477 (2020). https://doi.org/10.1186/s13104-020-05322-9

Download citation

Keywords

  • Bacterial cell cycle
  • Chromosome segregation
  • Bacillus subtilis
  • Single cell analyses