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Table 1 Strains and plasmids used in this study

From: A plasmid-encoded papB paralogue modulates autoaggregation of Escherichia coli transconjugants

Designation Description Reference or source
Strains   
 M63c E. coli wt, SR, NalR, SulR, AmpR, CipR, TeR, WR, KR [20]
 DH5α F ø80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK mK+) phoA supE44 λ–thi-1 gyrA96 relA1 Invitrogen
 ORN172 thr-l leuB thi-1 A(argF-lac)U169 maL1l xyl-7 ara-13 26 mtl-2 gal-6 rpsL tonA2 supE44 Δ(fimBEACDFGH); [24]
 EC1502 Rifampicin-resistant, plasmid free E. coli strain University of Bradford
Plasmids   
 pMB2 125 Kb Naturally occurring aac(6′)-Ib-cr-bearing plasmid [6]
 pMB80-2 Naturally occurring conjugative plasmid from enteropathogenic E. coli strain [25]
 pRMKO 93 Kb Miniplasmid constructed by deleting a 32,331 bp Not1—Xba1 fragment from parental plasmid pMB2 [6]
 pRMC pBluescript II SK containing a 32,331 bp Not1—Xba1 fragment from pMB2 cloned [6]
 pBAD/Thio-TOPO Arabinose inducible expression vector Invitrogen
 pBluescript II SK +  High copy number cloning vector Agilent
 pINKpefB papB/pefB paralogue from pMB2 cloned into pBAD/Thio-TOPO This study
 pLMJ50 90 Kb aggregative adherence plasmid from enteroaggregative E. coli strain 60A marked with a cat cassette [31]