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A plasmid-encoded papB paralogue modulates autoaggregation of Escherichia coli transconjugants
BMC Research Notes volume 13, Article number: 565 (2020)
Plasmids are key to antimicrobial resistance transmission among enteric bacteria. It is becoming increasingly clear that resistance genes alone do not account for the selective advantage of plasmids and bacterial strains that harbor them. Deletion of a 32 Kb fitness-conferring region of pMB2, a conjugative resistance plasmid, produced a hyper-autoaggregation phenotype in laboratory Escherichia coli. This study sought to determine the genetic basis for hyper-autoaggregation conferred by the pMB2-derived mini-plasmid.
The 32 Kb fragment deleted from pMB2 included previously characterized nutrient acquisition genes as well as putative transposase and integrase genes, a 272 bp papB/ pefB-like gene, and several open-reading frames of unknown function. We cloned the papB/ pefB paralogue and found it sufficient to temper the hyper-autoaggregation phenotype. Hyper-autoaggregation conferred by the mini-plasmid did not occur in a fim-negative background. This study has identified and characterized a gene capable of down-regulating host adhesins and has shown that trans-acting papB/pefB paralogues can occur outside the context of an adhesin cluster. This plasmid-mediated modification of a bacterial host’s colonization program may optimize horizontal transfer of the mobile element bearing the genes.
Bacterial autoaggregation is an adherence phenotype that can manifest in macroscopical clumping. Autoaggregation enhances colonization of some niches but because it is not always advantageous, it is typically regulated transcriptionally or post-transcriptionally by antiaggregation proteins or steric hindrance [1,2,3,4,5]. The Escherichia coli pangenome contains hundreds of known autoaggregation/antiaggregation factors, many of which also function in adherence to other cells and surfaces and have been studied in the context of specific virulent E. coli strains or pathotypes .
We recently isolated and sequenced a large multi-drug resistance plasmid from a commensal Escherichia strain in Nigeria and mapped a 32 Kb segment that ameliorated the 125 Kb plasmid’s carrying cost . Here we report that deletion of this portion of plasmid pMB2 produces the smaller pRMKO plasmid that is sufficient to enhance autoaggregation in laboratory E. coli strains in a manner that pMB2 does not. We further demonstrate that the responsible locus was an orphan papB/pefB paralogue, without a cognate fimbrial cluster, and propose that this gene may optimize colonization and transmission in conjugation recipients.
The canonical, pathogenicity-island-encoded PapB regulator mediates crosstalk among different fimbriae in extraintestinal pathogenic E. coli, leveraging type I fimbrial phase variation to ensure that type I fimbriae and pyelonephritis-associated (P)-fimbriae are not expressed simultaneously [7, 8]. Type I fimbriae or fim genes are present in most E. coli whilst the P-fimbriae-encoding pap genes are typically only found in uropathogenic and other extraintestinal E. coli. ON to OFF phase variation of type I fimbriae is mediated by FimE recombination at the fimS switch locus within the fim cluster. FimB, an analogous site-specific recombinase, predominantly mediates the reverse fimS phase OFF to phase ON switching . FimB switching behavior is repressed by PapB and PapB also promotes fimE expression, thereby shutting type I fimbriae OFF. Although papB lies within the chromosomal pyelonephritis-associated pilus (pap) cluster PapB controls fim expression even when expressed in trans from a plasmid in the laboratory [8, 10].
A number of PapB paralogues (PFAM03333) have been described in enteric bacteria, with varying fim regulatory activities. The family includes DaaA of the F1845 fimbrial cluster, SfaB of S-fimbriae, ClpB of CS31 fimbriae, FaeB of K88 fimbriae, FanA and FanB of K99 fimbriae, AfaA of the afa adhesin cluster as well as PefB of Salmonella pef fimbrial cluster [8, 11]. All these genes occur in the context of an adhesin gene cluster that they regulate and have the potential to crosstalk to other fimbriae.
Strains and plasmids
Bacterial strains (Table 1) were cultured in in Luria broth containing chloramphenicol (30 μg/mL), ampicillin (100 μg/mL), tetracycline (25 μg/mL), or neomycin (50 μg/mL), where required for selection, and maintained at − 70 °C in Luria broth:glycerol 1:1. Plasmid pMB2, originally isolated from an Escherichia isolate in Nigeria , and its derivatives are described in Table 1.
General microbiology and molecular biology procedures
Standard molecular biology procedures were used . pMB2, pRMKO and pRMC were extracted using the Qiagen® Large Construct Kit, as previously described . All other plasmid extractions used the Qiagen® MiniPrep Kit. Plasmids were electroporated into E. coli strains using a Bio-Rad micropulser. The pMB2 papB/pefB analogue was amplified using the oligonucleotides 5′CACCACTCCCTCCCCCTATCCAA-3′ and 5′-CTCACGGTAGAAATATTTAAGAGC-3′ and then cloned into pBAD/Thio-TOPO. Overexpression of a protein of size consistent with PapB by 1, 2 and 4% arabinose, and repression by 1% glucose was verified by SDS PAGE according to the pBAD/Thio-TOPO manufacturer’s protocols (Additional file 1: Figure S1). In vitro conjugation was effected by solid mating on LB agar as described previously. The number of transconjugant colonies per donor colony-forming units was computed as the plasmid transfer efficiency .
Sequence was viewed in Artemis  and BLAST  searches were performed via Artemis on the NCBI platform. Multiple alignments were performed using Clustal W on the EBI server  and in MEGA6 . Maximum likelihood phylogenies were computed in MEGA6.
The settling assay of Hasman et al. , with previously detailed slight modifications , was used to quantify autoaggregation. Overnight cultures were prepared in test medium containing selective antibiotics and where necessary, gene expression was induced from the arabinose promoter in the pBAD/Thio-TOPO vector with 1% arabinose for 90 min before the start of the assay. At the assay start, cultures of each strain were adjusted to the same optical density at 600 nm (OD600). Eight mL of each adjusted culture was placed into paired tubes. One tube in each pair remained static and the other was lightly vortexed before each optical density measurement. The tubes were incubated without shaking at 37 °C. At designated time points, 0.5 mL was removed from within 2 cm of the surface of the culture, and the OD600 was measured, diluting the sample if required. OD600 supernatant measurements for different strains were compared using a t-test.
Results and discussion
A PapB/PefB paralogue on pMB2 interferes with autoaggregation
pMB2 was originally extracted from a commensal Escherichia isolate from Nigeria [20, 21]. In the course of functional analyses of fitness loci , we additionally observed that DH5α carrying the pRMKO miniplasmid autoaggregated significantly, particularly under low nutrient conditions. As shown in Fig. 1a, autoaggregation in liquid media was prominent in DH5α (pRMKO) but absent in DH5α (pMB2) cultures grown under the same conditions (p < 0.001). Cloning the 32 Kb deleted region into pBluescript also reduced autoaggregation of DH5α compared to an isogenic strain carrying the vector alone (Fig. 1a). We examined the sequence of the region deleted from pRMKO for genes with the potential to contribute to autoaggregation. The best candidate is small open reading frame (orf) located from 11,258–11,530 of the plasmid. The predicted amino acid sequence of this gene was 29% identical/50% similar to E. coli PapB from uropathogenic strain J96 (Genbank Accession ELL39276.1) and was 32% identical/ 48% similar to PefB, a PapB paralogue from Salmonella enterica serovariety Typhimurium strain LT2 (Genbank Accession AAL23523). There are no fimbrial genes proximal to the pMB2 papB-like gene, however there are mobility genes on either side and the papB-like gene region has a noticeably lower G + C content than the surrounding region (Fig. 1b).
We cloned the papB/pefB paralog under the control of the arabinose promoter and measured the clone’s ability to complement pRMKO hyperautoaggregation. As shown in Fig. 1c, when the papB/pefB paralogue is induced by arabinose, autoaggregation was diminished in DH5α (pRMKO, pINKPefB). This phenotype was not seen when the arabinose promoter is glucose-repressed (Fig. 1c). Based on this finding, we attribute the hyperautoaggregation phenotype conferred by pRMKO to deletion of the pMB2 papB/pefB paralogue.
The pMB2 papB/pefB paralogue acts on chromosomally-encoded effectors
PapB binds between papI and papB in the extraintestinal pathogenic E. coli pap cluster as well as, if less strongly, upstream of fimE . R61A and C65A substitutions, within a hydrophilic region of PapB, produce derivatives unable to bind DNA . As shown in Fig. 2, pMB2 PefB paralogue has a cysteine at the position equivalent to 65 in PapB. It does not have an arginine at position 61 but does have a positively-charged lysine residue at the equivalent position. Positions 86 and 91 at the PapB carboxy terminus are also essential for switching activity . Holden et al. reported that L82F or F83Q substitutions, which altered these PapB residues to the equivalents in the non-binding DaaA paralogue, reduced switching. When both substitutions were made, activity was obliterated . As shown in Fig. 2, the pMB2 PefB paralogue has a leucine at the position equivalent to PapB 82 but has an asparagine substitution at position 83, and so, even though it is considerably dissimilar to PapB, it could conceivably inhibit fim switching. However, Fig. 2 illustrates that there are many sequence differences between the allele we have identified and previously studied members of this family making it impossible to infer the nature and degree of its function or activity from sequence alone. In DH5α (pMB2), PapB/PefB could be repressing either chromosomally-encoded fim genes or pMB2 factors not deleted from pRMKO. From the complete sequence of pMB2, we did not identify genes encoding chaperone-user fimbriae or any other fimbrial type other than the F (conjugative)-pili, which are known to promote autoaggregation [6, 22, 23], although they lack a type I fimbriae-like switch. We additionally acknowledge that any of the several pMB2 orfs of unknown function could contribute to autoaggregation. To determine which possibility could be at play, we transformed pMB2 and pRMKO into ORN172, a fim-negative E. coli strain . In this Δfim background, there were no papB/pefB-associated differences in autoaggregation (Fig. 1d). Therefore, while it remains to be confirmed in direct experiments, this finding suggests that interactions of papB/pefB with core chromosomal factors, likely fimbriae, account for the hyperautoaggregation seen in DH5α (pRMKO) and that the hydrophobic residue at position 83 of PapB, may be less critical for FimB regulation than the presence of a leucine at position 82.
PapB is not known to directly regulate conjugative pili, but a fim repressor could conceivably enhance F-pilus-mediated autoaggregation and conjugation by preventing occlusion by chromosomally-encoded fimbriae. We performed an initial test of this hypothesis by comparing conjugative transfer rates from DH5α (pMB2) and DH5α (pRMKO) to strain EC1502. (The pRMKO miniplasmid contains the complete conjugative gene region of parental pMB2 plasmid). We recorded a transfer rate of 7.9 × 10–6 for DH5α (pMB2) in solid matings, compared to 3 × 10–5 of control plasmid pMB80 . In contrast, conjugation from DH5α (pRMKO) could not be detected at the limits of the assay. Due to the low conjugation rate overall and the impossibility of detecting rates below 1 × 10–8 we could not perform a robust complementation experiment. However, altogether the available data support the idea that papB/pefB repressed genes could interfere with pMB2 conjugative transfer.
The pMB2 papB/pefB paralogue is widely distributed and largely overlooked on extraintestinal E. coli plasmids
A BLAST search of the pMB2 nucleotide sequence range 7247–14,962, representing the papB/ pefB paralogue, its flanking low G + C region and the adjacent NikM/Hmu genes, reveal that it is a common plasmid-borne feature. Importantly, plasmids of E. coli O25b:H4-ST131 strains, commonly implicated in bloodstream, urinary and other extraintestinal infections, bear the entire region depicted in Fig. 1b. On the E. coli str. UMN026 plasmid p1ESCUM, often used an extraintestinal E. coli reference sequence (GenBank Accession Number: CU928148.1) , the entire region is present, but the papB/pefB paralog is not annotated as is the case for some well-known resistance plasmids , but not for others.
Cystitis and pyelonephritis isolates are more likely to contain one or more pap operons than other E. coli. Holden et al. , who observed this, also showed that cystitis and pyelonephritis isolates are better able to agglutinate red blood cells and attributed this to a higher copy-number and activity of intracellular PapB, which promoted expression of alternate fimbriae whilst shutting type I fimbriae OFF. papB/pefB paralogues on plasmids could achieve this effect irrespective of the presence of a full fimbrial operon consequently modulating the expression of core adhesins for in vivo adherence and/or exposure to the immune system. Thus, virulence factor profiling of pathogenic E. coli needs to take into account the available fimbrial genes as well as fimbrial regulators some of which—like the gene we characterize—could be plasmid-borne. As our papB/pefB paralogue is borne on a plasmid conferring resistance to no less than eight antimicrobial classes, antibiotic selective pressure could ultimately alter colonization and virulence profiles of strains in the wild.
In conclusion, pMB2 is self-transmissible by an F-type conjugative system that may function more effectively due to repression of chromosomal adhesins by a papB/pefB paralogue located on the same plasmid. Plasmids almost identical to pMB2 have been isolated from the USA (Accession number CP054458), Colombia (Acession number CP010372), France (Accession number LO017738), the UK (Accession number CP023372, CP018990) and elsewhere. The papB/pefB paralogue we describe is highly conserved among them and therefore the autoaggregation it represses is likely common, warranting further study. Our findings add to experimentally validated reports of two other non-resistance loci of pMB2 that promote E. coli survival and adaptability [6, 29]. Thus there are multiple genes resistance plasmids hat compensate the costs of their carriage or, as in this case, potentially propagate transmission. This study also provides yet another example of  horizontally acquired DNA that radically affects the expression of recipient cell core genes.
This study used convincing indirect methods to infer a regulatory link between the papB/pefB paralog on pMB2 and chromosomal adhesins and our findings agree with existing evidence on the mechanism of action of genes that belong to this family. We however lack the resources to demonstrate that the new papB/pefB paralog directly interacts with cis or trans fimbrial regulatory elements, or that fimbrial production is altered by the presence or absence of the papB/pefB gene.
Availability of data and materials
The sequence of plasmid pMB2, on which this manuscript is based is available from Genbank, Accession number MK492688. Other data generated or analysed during this study are included in this article.
Schembri MA, Dalsgaard D, Klemm P. Capsule shields the function of short bacterial adhesins. J Bacteriol. 2004;186(5):1249–57.
Blanton LV, Wang LT, Hofmann J, DuBow J, Lafrance A, Kwak S, Bowers L, Levine MA, Hale CO, Meneely PM, Okeke IN. Aggregative Adherence and Intestinal Colonization by Enteroaggregative Escherichia coli Are Produced by Interactions among Multiple Surface Factors. mSphere. 2018;3(2):e00078–18.
Schembri MA, Hjerrild L, Gjermansen M, Klemm P. Differential expression of the Escherichiacoli autoaggregation factor antigen 43. J Bacteriol. 2003;185(7):2236–42.
Trunk T, Khalil HS, Leo JC. Bacterial autoaggregation. AIMS Microbiol. 2018;4(1):140–64.
Sheikh J, Czeczulin JR, Harrington S, Hicks S, Henderson IR, Le Bouguenec C, Gounon P, Phillips A, Nataro JP. A novel dispersin protein in enteroaggregative Escherichiacoli. J Clin Investig. 2002;110(9):1329–37.
Monárrez R, Braun M, Coburn-Flynn O, Botelho J, Odetoyin BW, Otero-Vera JI, Quartey NKE, Peixe L, Aboderin AO, Okeke IN. A large self-transmissible resistance plasmid from Nigeria contains genes that ameliorate a carrying cost. Sci Rep. 2019;9(1):19624.
Nowicki B, Rhen M, Vaisanen-Rhen V, Pere A, Korhonen TK. Organization of fimbriate cells in colonies of Escherichiacoli strain 3040. J Gen Microbiol. 1985;131(5):1263–6.
Holden NJ, Uhlin BE, Gally DL. PapB paralogues and their effect on the phase variation of type 1 fimbriae in Escherichiacoli. Mol Microbiol. 2001;42(2):319–30.
Klemm P. Two regulatory fim genes, fimB and fimE, control the phase variation of type 1 fimbriae in Escherichiacoli. Embo J. 1986;5(6):1389–93.
Xia Y, Gally D, Forsman-Semb K, Uhlin BE. Regulatory cross-talk between adhesin operons in Escherichiacoli: inhibition of type 1 fimbriae expression by the PapB protein. EMBO J. 2000;19(7):1450–7.
Xia Y, Uhlin BE. Mutational analysis of the PapB transcriptional regulator in Escherichiacoli. Regions important for DNA binding and oligomerization. J Biol Chem. 1999;274(28):19723–30.
Sambrook J, Russell DW. Molecular cloning : a laboratory manual. 3rd ed. Cold Spring Harbor: Cold Spring Harbor Laboratory Press; 2001.
Lu J, Manchak J, Klimke W, Davidson C, Firth N, Skurray RA, Frost LS. Analysis and characterization of the IncFV plasmid pED208 transfer region. Plasmid. 2002;48(1):24–37.
Carver T, Harris SR, Berriman M, Parkhill J, McQuillan JA. Artemis: an integrated platform for visualization and analysis of high-throughput sequence-based experimental data. Bioinformatics. 2012; 28(4):464–9.
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990;215(3):403–10.
Madeira F, Park YM, Lee J, Buso N, Gur T, Madhusoodanan N, Basutkar P, Tivey ARN, Potter SC, Finn RD, et al. The EMBL-EBI search and sequence analysis tools APIs in 2019. Nucleic Acids Res. 2019;47(W1):W636–41.
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol. 2013.
Hasman H, Chakraborty T, Klemm P. Antigen-43-mediated autoaggregation of Escherichiacoli is blocked by fimbriation. J Bacteriol. 1999;181(16):4834–41.
Bhargava S, Johnson BB, Hwang J, Harris TA, George AS, Muir A, Dorff J, Okeke IN. The heat resistant agglutinin 1 is an accessory enteroaggregative Escherichiacoli colonization factor. J Bacteriol. 2009;191:4934–42.
Odetoyin BW, Hofmann J, Aboderin AO, Okeke IN. Diarrhoeagenic Escherichiacoli in mother-child Pairs in Ile-Ife, South Western Nigeria. BMC Infect Dis. 2016;16(1):1.
Odetoyin BW, Labar AS, Lamikanra A, Aboderin AO, Okeke IN. Classes 1 and 2 integrons in faecal Escherichiacoli strains isolated from mother-child pairs in Nigeria. PLoS ONE. 2017;12(8):e0183383.
Ghigo JM. Natural conjugative plasmids induce bacterial biofilm development. Nature. 2001;412(6845):442–5.
Reisner A, Krogfelt KA, Klein BM, Zechner EL, Molin S. In vitro biofilm formation of commensal and pathogenic Escherichiacoli strains: impact of environmental and genetic factors. J Bacteriol. 2006;188(10):3572–81.
Woodall LD, Russell PW, Harris SL, Orndorff PE. Rapid, synchronous, and stable induction of type 1 piliation in Escherichia coli by using a chromosomal lacUV5 promoter. J Bacteriol. 1993;175(9):2770–8.
Nwaneshiudu AI, Mucci T, Pickard DJ, Okeke IN. A second large plasmid encodes conjugative transfer and antimicrobial resistance in O119:H2 and some typical O111 enteropathogenic Escherichia coli strains. J Bacteriol. 2007;189(16):6074–9.
Lescat M, Calteau A, Hoede C, Barbe V, Touchon M, Rocha E, Tenaillon O, Medigue C, Johnson JR, Denamur E. A module located at a chromosomal integration hot spot is responsible for the multidrug resistance of a reference strain from Escherichiacoli clonal group A. Antimicrob Agents Chemother. 2009;53(6):2283–8.
Li J-J, Spychala CN, Hu F, Sheng J-F, Doi Y. Complete nucleotide sequences of blaCTX-M-harboring IncF plasmids from community-associated Escherichiacoli strains in the United States. Antimicrobial Agents Chemother. 2015;59(6):3002–7.
Holden NJ, Totsika M, Mahler E, Roe AJ, Catherwood K, Lindner K, Dobrindt U, Gally DL. Demonstration of regulatory cross-talk between P fimbriae and type 1 fimbriae in uropathogenic Escherichia coli. Microbiology. 2006;152(Pt 4):1143–53.
Billard-Pomares T, Clermont O, Castellanos M, Magdoud F, Royer G, Condamine B, Fouteau S, Barbe V, Roche D, Cruveiller S, et al. The arginine deiminase operon is responsible for a fitness trade-off in extended-spectrum-β-lactamase-producing strains of Escherichia coli. Antimicrob Agents Chemother. 2019;63(8):e00635–19.
Doyle M, Fookes M, Ivens A, Mangan MW, Wain J, Dorman CJ. An H-NS-like stealth protein aids horizontal DNA transmission in bacteria. Science. 2007;315(5809):251–2.
Joo LM, Macfarlane-Smith LR, Okeke IN. Error-prone DNA repair system in enteroaggregative Escherichia coli identified by subtractive hybridization. J Bacteriol. 2007;189(10):3793–803.
We thank Lawrence Wang for helpful comments and Mandy Levine for technical assistance.
INO is an African Research Leader award supported by the UK Medical Research Council (MRC) and the UK Department for International Development (DFID) under the MRC/DFID Concordat agreement that is also part of the EDCTP2 programme supported by the European Union. This work was supported by a Branco Weiss Fellowship from the Society in Science, Zürich, Switzerland and by US National Science Foundation ‘Research at Undergraduate Institutions’ award MCB 0948460 both to INO, and by an undergraduate research studentship from Haverford College to RM.
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. Coomassie blue-stained SDS PAGE gel of whole cell lysates of DH5α pRMKO transformed with pINKpefB and grown in LB containing 0, 1, 2 and 4 % arabinose or 1% glucose showing induction of a protein of papB/pefB predicted size (7 KDa). Ladder: Prestained protein marker (Biorad).
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Monárrez, R., Okeke, I.N. A plasmid-encoded papB paralogue modulates autoaggregation of Escherichia coli transconjugants. BMC Res Notes 13, 565 (2020). https://doi.org/10.1186/s13104-020-05405-7
- Fimbrial regulation