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Table 1 Species level taxonomic assignment successa of barcodes from different primer pairs of selected bee species representing different families

From: A new primer pair for barcoding of bees (Hymenoptera: Anthophila) without amplifying the orthologous coxA gene of Wolbachia bacteria

Taxon LCO1490/HCO2198 BeeCox1F1/BeeCox1R2
Megachilidae
 Anthidium punctatum (107)b CORRECT CORRECT
 Chelostoma florisomne (99) CORRECT CORRECT
 Coelioxys alata (1) CORRECT CORRECT
 Coelioxys elongata (110) CORRECT CORRECT
 Coelioxys rufescens (108) CORRECT CORRECT
 Herides truncorum (112) CORRECT CORRECT
 Osmia bicolor (93) CORRECT CORRECT
 Megachile circumcincta (2) CORRECT CORRECT
 Megachile willughbiella (104) CORRECT CORRECT
 Megachile versicolor (95) CORRECT CORRECT
Andrenidae
 Andrena barbilabris (4) CORRECT CORRECT
 Andrena flavipes (6) WOLBACHIA CORRECT
 Andrena fulvago (105) MIXED CORRECT
 Andrena ovatula (5) MIXED CORRECT
 Andrena ovatula (7) WOLBACHIA CORRECT
 Andrena minutula (15) CORRECT CORRECT
 Andrena nigroaenea (8) WOLBACHIA CORRECT
 Andrena wilkella (35) MIXED CORRECT
 Andrena wilkella (36) MIXED CORRECT
 Andrena wilkella (37) MIXED CORRECT
Halictidae
 Lasioglossum minutulum (14) MIXED CORRECT
 Lasioglossum nitidiusculum (41) CORRECT CORRECT
 Lasioglossum pauxillum (49) CORRECT CORRECT
 Lasioglossum semilucens (46) WOLBACHIA CORRECT
 Lasioglossum sexstrigatum (16) CORRECT CORRECT
 Lasioglossum villosulum (48) CORRECT CORRECT
 Sphecodes ferruginatus (97) CORRECT CORRECT
 Sphecodes miniatus (19) CORRECT CORRECT
 Sphecodes monilicornis (18) WOLBACHIA CORRECT
 Sphecodes puncticeps (17) CORRECT CORRECT
Colletidae
 Colletes daviesanus (117) WOLBACHIA CORRECT
 Hylaeus brevicornis (13) CORRECT CORRECT
 Hylaeus communis (12) WOLBACHIA CORRECT
 Hylaeus confusus (106) CORRECT CORRECT
Apidae
 Anthophora furcata (102) CORRECT CORRECT
 Bombus pratorum (101) CORRECT CORRECT
 Bombus rupestris (91) CORRECT CORRECT
 Bombus terrestris (72) CORRECT CORRECT
 Ceratina cyanea (113) MIXED CORRECT
 Epeolus variegatus (115) CORRECT CORRECT
 Eucera nigrescens (114) CORRECT CORRECT
 Nomada fabriciana (96) CORRECT CORRECT
 Nomada goodeniana (92) CORRECT CORRECT
 Nomada marshamella (98) CORRECT CORRECT
 Nomada ruficornis (118) MIXED CORRECT
 Nomada succincta (71) MIXED CORRECT
Mellitidae
 Melitta haemorrhoidales (109) CORRECT CORRECT
 Melitta leporina (M10) MIXED CORRECT
  1. aAssignment success was either given as unambiguous (correct) identification in BOLD as CORRECT, misamplification of endosymbiont as WOLBACHIA, or a sequence that showed double or mixed signal and could not be identified as MIXED
  2. bNumber in parentheses correspond to unique identifiers with the online available sequence chromatograms and data