Proximity ligation assay to detect DUX4 protein in FSHD1 muscle: a pilot study
BMC Research Notes volume 15, Article number: 163 (2022)
Aberrant expression in skeletal muscle of DUX4, a double homeobox transcription factor, underlies pathogenesis in facioscapulohumeral muscular dystrophy (FSHD). Although previous studies of FSHD muscle biopsies detected mRNAs encoding DUX4 and its target genes, no studies had reported detection of DUX4 protein. Our objective was to develop a proximity ligation assay (PLA) for DUX4 and to determine if this assay could detect DUX4 protein in FSHD muscle sections.
We developed a PLA protocol using two DUX4 antibodies previously reported by Stephen Tapscott’s group: P2G4, a mouse mAb specific for an epitope in the N-terminal region, and E5-5, a rabbit mAb specific for an epitope in the C-terminal region, in combination with commercial PLA secondary reagents. We validated the DUX4 PLA using cultured human myogenic cells in which DUX4 was ectopically expressed in a small fraction of nuclei. Using this two primary mAb PLA on an FSHD1 biceps biopsy, we observed nuclei with apparent DUX4 PLA signals associated with a small subset of myofibers (~ 0.05–0.1%). Though a limited pilot study, these results suggest that the two primary mAb PLA protocol could be useful for detecting DUX4 protein in FSHD muscle biopsies.
Aberrant expression in skeletal muscle of DUX4, a double homeobox transcription factor, underlies pathogenesis in facioscapulohumeral muscular dystrophy (FSHD) [1,2,3,4,5]. In the two forms of the disease—FSHD1 (~ 95% of cases) and FSHD2 (~ 5% of cases)—DUX4 is expressed from an open reading frame in the most telomeric 3.3 kb D4Z4 repeat on chromosome 4q [6,7,8], with rare cases transcribed from chromosome 10q . In differentiated cultures of myogenic cells obtained from FSHD1 patients, DUX4 protein can be detected by immunostaining in only a small fraction of myotube nuclei, typically 0.01–0.1% [7, 10,11,12,13,14,15]. In FSHD muscle biopsies, low levels of DUX4 mRNA, as well as mRNAs encoding DUX4 target genes, have been detected . However, no previous studies had reported detection of DUX4 protein in FSHD muscle biopsies. The objectives of this pilot study, therefore, were to (i) develop and validate in cells a proximity ligation assay (PLA) [17,18,19] for DUX4 and (ii) determine if this assay could detect DUX4 protein in an FSHD1 muscle biopsy. We used PLA because of the high specificity and low background that can be achieved with this technique [17,18,19]. The results, though limited and requiring replication and expansion, suggest that PLA could be of interest as a useful technique to detect DUX4 protein in sections of biopsied FSHD muscle.
Methods and materials
Human primary myogenic cells (21Ubic) were from an unaffected donor, and previous publications have described institutional approvals, isolation, culture conditions, and authentication protocols for these cells [11,12,13, 15, 20]. Also see Declarations.
The FSHD1 biceps biopsy (#6524) was from a 17 year-old male donor with a very short 16 kb (~ 5 repeats), D4Z4 length. The unaffected biceps biopsy (#9557) was from a 33 year-old female donor. Biopsies were provided by Dr. Marina Mora and Dr. Maurizio Moggio (Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy) through the Telethon Network of Genetic Biobanks and were maintained frozen at − 80 °C until sectioned. As noted further under Declarations, biopsies were collected under approved institutional protocols.
Two mammalian baculovirus (BacMam) vectors were used. To express full-length DUX4 tagged with a C-terminal V5 epitope in myoblasts, we used a modified mammalian baculovirus, termed BacMam-DUX4-V5, as described [13, 15, 21, 22]. As a negative control, we infected parallel cultures with a separate mammalian baculovirus, termed BacMam-GFP [13, 15], which leads to ectopic expressed of GFP. For both baculovirus vectors expression of the ectopic protein was under control of the human CMV-IE1 promoter. The amount of BacMam-DUX4-V5 or BacMam-GFP added to cultures was adjusted so that expression of DUX4 or GFP occurred in ~ 1–2% of the cells in cultures of human primary myoblasts.
Two DUX4 antibodies, both previously generated by Geng et al. , were used in this study: (i) P2G4 (a gift of Dr. Stephen Tapscott) which is a mouse mAb specific for an epitope in the N-terminal region; and (ii) E5-5 (cat. ab124699, Abcam, Boston MA), which is a rabbit mAb specific for an epitope in the C-terminal region. Immunostaining for the V5 epitope or GFP were as described [13, 15, 22, 24].
Preparation of cells for proximity ligation assay
Undifferentiated 21Ubic myoblasts were grown on porcine gelatin-coated, 4-well permanox chamber slides (cat.177437, Thermo Fisher, Waltham MA) and incubated for 48 h with BacMam-DUX4-V5 or BacMam-GFP, after which the cultures were fixed in 2% paraformaldehyde in PBS for 10 min at room temperature [13, 15]. After three 5 min washes with PBS, PBS plus 10 mM glycine, and PBS, the cultures were quickly rinsed with deionized water. The fixed cultures were permeabilized in 0.5% Triton X-100 in PBS and then incubated for 30 min at 37 °C in blocking solution, which consisted of 4% normal goat serum (Vector Labs, Burlingame CA), 4% normal horse serum (Vector Labs), 4% bovine serum albumin (Sigma-Aldrich, St. Louis MO), and 0.1% Triton X-100 (Sigma-Aldrich) in PBS. The fixed and blocked cells were incubated overnight at 4 °C with both the N-terminal P2G4 mouse mAb and the C-terminal E5-5 rabbit mAb, each diluted 1:250 and mixed together in blocking solution.
Preparation of muscle sections for proximity ligation assay
Frozen biopsies were embedded in Sakura Tissue-Tek O.C.T. (cat. 27050, Ted Pella Inc. Redding CA), and, using a cryostat, 10 µm serial sections were made from each biopsy and collected on ten glass slides at three sections per slide. Slides were air-dried for 30 min and each section was encircled with a Pap Pen (cat. H4000, Vector Labs) to make a well for reagents. Encircled sections were rehydrated in PBS for 10 min, fixed in ice-cold 100% methanol for 10 min, washed for three times at 5 min each with PBS, washed once quickly with deionized water, and then incubated in blocking solution for 30 min at 37 °C. The fixed and blocked muscle sections were incubated overnight at 4 °C with both the N-terminal P2G4 mouse mAb and the C-terminal E5-5 rabbit mAb, each diluted 1:250 and mixed together in blocking solution.
Proximity ligation assay
After overnight incubation at 4 °C with the mixture of mouse P2G4 and rabbit E5-5 mAbs, cells or sections were washed and processed for PLA exactly according to the manufacturer’s published protocols [25, 26]. PLA reagents, all from Sigma-Aldrich (St. Louis MO), included the following:
DUO92004. Duolink In Situ PLA Probe Anti-Mouse MINUS, Affinity purified Donkey anti-Mouse IgG (H + L) (lot A40108).
DUO92002. Duolink In Situ PLA Probe Anti-Rabbit PLUS, Affinity purified Donkey anti-Rabbit IgG (H + L) (lot A40406).
DUO92008. Duolink In Situ Detection Reagents Red. This reagent kit included (i) 5× Ligation, which contained oligonucleotides that hybridized to the PLA probes and components needed for ligation; (ii) 1× Ligase (1 unit/μL); (iii) 1× Polymerase (10 units/μL); (iv) 5× Amplification Red, which contained components needed for rolling circle amplification and oligonucleotide probes labeled with red fluorophore that hybridized to the amplification product.
DUO82049. Duolink in situ wash buffers A and B.
DUO82040. Duolink in situ mounting medium with DAPI.
Images of cells and sections were acquired using a 20× or 40× objective on a Nikon E800 microscope equipped with a Spot camera and software version 5.1 (Diagnostic Instruments Inc., Sterling Heights, Michigan).
Results and discussion
We first used the two mAb PLA protocol (as described in Methods) on primary human myoblasts that had been incubated for 48 h with an amount of BacMam-DUX4-FL-V5 that, based on V5 immunocytochemistry, generated DUX4 expression in a small percentage (~ 1–2%) of the host cells. In such cultures, we found a similarly small percentage of myoblast nuclei that showed a positive PLA signal for DUX4 (Fig. 1 and Additional file 1: Fig. S1). These nuclear signals had the characteristic pattern of small fluorescent puncta expected for PLA [17, 18]. Background staining (i.e., non-nuclear) was almost absent (Fig. 1 and Additional file 1: Fig. S1). The PLA signal was inhomogeneously distributed within most nuclei, with different regions showing lower or higher densities of PLA puncta. The degree of intra-nuclear signal inhomogeneity varied among nuclei (e.g. compare Fig. 1B–F). Standard immunofluorescence assays with mAb E5-5 also show inhomogeneity in DUX4 distribution within many individual nuclei [13, 15, 24]. As controls, we noted that nuclear PLA signals were not found when (i) mouse mAb P2G4 was replaced with an anti-GFP antibody or (ii) the myoblasts were incubated with BacMam-GFP instead of BacMam-DUX4-FL-V5. Thus, the two primary mAb PLA protocol showed low background and high specificity for BacMam-mediated DUX4 protein expression in myoblasts.
Encouraged by these PLA results with myoblasts, we next used the two primary mAb PLA protocol on sections of FSHD1 and unaffected muscle biopsies (see Methods). Histological analyses of sections from the FSHD1 biopsy (Additional file 1: Fig. S2) showed minimal myopathic changes , including a small percentage of angular (possibly atrophic) myofibers, as well as occasional central nucleate myofibers. There was little or no evidence of extensive fiber rounding, perivascular inflammation, or fatty replacement.
When examining PLA signals on sections of this FSHD1 biceps muscle, we observed a small number of nuclei with PLA signals that were well above background and entirely within the nuclear boundary (Figs. 2, 3). In some nuclei, the PLA signal was evidently composed of multiple puncta (e.g. Figs. 2D, 3C), though, in others, puncta in the PLA signal were not well-resolved. When compared to PLA of myoblasts (Fig. 1), the individual PLA puncta in sections were generally less-well resolved, perhaps due to the varied orientations and greater thickness of muscle nuclei vs. the nuclei in cultured myoblasts. Also, nuclei with positive PLA signals were located at different depths in the sections, so different image planes were needed to visualize puncta when more than one PLA-positive nucleus was present within a single 40X microscope field (Additional file 1: Fig. S3). The methanol fixation used for the muscle sections also may have limited resolution vs. the paraformaldehyde fixation used for myoblasts.
On sections analyzed by PLA, the non-nuclear signals were generally low, consisting of scattered individual puncta, diffuse fluorescence in myofibers damaged during sectioning, and, on a minority of myofibers, regions of the cell border (Additional file 1: Fig. S4). When we analyzed sections of the unaffected muscle biopsy by PLA, we observed similar non-nuclear background, but we did not observe any nuclei with PLA signals.
We enumerated myofibers and DUX4-positive nuclei on four sections and found that ~ 0.05–0.1% of the FSHD1 myofibers were associated with DUX4-positive nuclei (~ 8000 myofibers had 6 DUX4-positive nuclei).
The two primary mAb protocol we developed appears to have good specificity for DUX4 protein in nuclei coupled with a low background on both cultured myoblasts and muscle sections. In earlier preliminary work, we also attempted to analyze muscle sections with standard single (mAb E5-5) and double (mAbs E5-5 and P2G4) immunofluorescence, as well as with a variation of the PLA protocol that used only single primary mAb (E5-5) with two secondary mAbs. Despite rare detection of DUX4-positive nuclei, each of these alternatives was limited by much higher background, particularly at myofiber surfaces and along connective tissue, than was seen with the two primary mAb PLA protocol reported here. As described in the Limitations section, we recognize that this study needs to be extended and replicated. Although we are not able to continue these studies (due to P.I. retirement), we have presented our initial work both because detection of DUX4 protein in FSHD muscle samples has not previously been reported and because our PLA protocol could prove useful to other investigators in further studies of FSHD pathogenesis.
Additional experiments are needed to overcome the limitations of this n = 1 pilot study. In particular, to replicate and extend confidence in these initial results, the two primary mAb PLA should be carried out on biopsies from many additional FSHD and unaffected donors. Coupling DUX4 PLA and mRNA analyses of each biopsy could determine how closely correlated the number of DUX4-positive nuclei is to the level of DUX4 mRNA. In addition, positive results from PLA analyses of one or more of the mouse models in which human DUX4 is expressed [28,29,30,31,32] could increase confidence in specificity of the protocol. Co-staining the PLA sections for dystrophin by immunofluorescence could be used to determine if the DUX4-positive nuclei are located within myofibers. Future studies would also be strengthened by using confocal microscopy to increase resolution and to allow three-dimensional reconstruction of PLA signals within myonuclei.
Availability of data and materials
All data generated or analyzed during this study are included in this published article and its Additional file 1.
Proximity ligation assay
Facioscapulohumeral muscular dystrophy
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We thank Dr. Stephen Tapscott (Fred Hutchinson Cancer Research Center) for the gift of the N-terminal DUX4 mAb P2G4; Dr. Frederick M. Boyce (Massachusetts General Hospital) for generating and providing BacMam-DUX4-V5 and BacMam-GFP; and Michaela Welch (Boston University School of Medicine) for help with initial studies using PLA in our laboratory. Muscle biopsies were provided through the biobank: “Cells, tissues, and DNA from patients with neuromuscular diseases”, which is a member of the Telethon Network of Genetic Biobanks (project no. GTB12001, funded by Telethon Italy) and of the EuroBioBank network. In particular, we thank Dr. Marina Mora and Dr. Maurizio Moggio (Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy), as well as the anonymous tissue donors, for providing the FSHD1 and unaffected biceps biopsies through the biobank.
This work was supported by the Muscular Dystrophy Association [#606383 to J.B.M.]; the National Institutes of Health (NIH) [R01AR062587 to Peter L. Jones with a subcontract to J.B.M.]; and the FSHD Society [FSHS-82016 to S.H.].
Ethics approval and consent to participate
All human cells and biopsies were anonymized prior to our receipt with no personal identifying information available to us. In addition, the cells and biopsies had been produced prior to our study and had been collected under protocols approved by the appropriate institution that included informed donor consent and approval to publish results in accordance with standards of the Helsinki Declaration. Because our studies were of human materials that were obtained from biobanks and for which personal identification data were not obtainable by us, the studies were classified as exempt from Human Studies review by the Boston University Institutional Review Board in accordance with US Department of Heath and Human Services policy.
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Beermann, M.L., Homma, S. & Miller, J.B. Proximity ligation assay to detect DUX4 protein in FSHD1 muscle: a pilot study. BMC Res Notes 15, 163 (2022). https://doi.org/10.1186/s13104-022-06054-8
- Facioscapulohumeral muscular dystrophy
- Skeletal muscle biopsy
- Proximity ligation assay