Antibody screening data of human midgestation liver cells with a focus on hematopoietic, liver sinusoidal endothelial, and hepatoblast cell-populations
BMC Research Notes volume 15, Article number: 358 (2022)
Cell-surface antigen screening was performed on human fetal liver cells using flow cytometry. The goal was to provide proteomic expression data on a number of human fetal liver cell populations that can inform studies on developmental hepatology and hematology.
A 21 weeks’ gestation liver was depleted of erythrocytes prior to antibody staining. Screening was performed using phycoerythrin-labelled antibodies against 332 antigens. In addition to these antibodies, all samples were stained for CD14, CD45, CD235a, and CD326 (epithelial cell adhesion molecule – EpCAM). Subpopulations of fetal liver cells were identified using the co-stained antigens. Hematopoietic cells were identified by their expression of CD45 and CD235a; non-hematopoietic cells were further subdivided based on CD14 and CD326 expression. CD326++CD14low hepatoblasts and CD14++ liver sinusoidal endothelial cells were analyzed for the frequency and intensity of antigen expression. Analyzed flow cytometry data are presented for the expression of the antigens on hematopoietic cells and on non-hematopoietic cells in the context of CD14 and CD326 expression.
During prenatal development the cellular composition of the liver is complex as the liver is a major site of hematopoiesis while also supporting the development of the hepatocytic elements that dominate the adult liver . Moreover, there are notable differences between adult hepatocytes and their prenatal precursors known as hepatoblasts. To better distinguish and isolate different cell types for study, flow cytometric screening of cell surface antigens was performed to provide phenotype data for multiple cell types found in the human midgestation liver. One aim was to discover new antigens that are useful in distinguishing cell types, but confirmation of antigen expression only known from studies of human adult liver or liver cells of other species was also sought.
Five color flow cytometry was used to identify different subsets of live cells using three colors in addition to the antibody screening performed using phycoerythrin (PE) labeled antibodies. Hematopoietic cells were stained with a combination of CD45 to identify progenitors and mature leukocytes as well as CD235a to mark any remaining erythrocytes not depleted from the samples. CD326, although expressed by many different cell types in the fetal liver , was used to identify hepatoblasts [3, 4]. CD14 expression further distinguished hepatoblasts as well as liver sinusoidal endothelial cells (LSECs) [2, 5].
These data may be of value to researchers studying liver ontogeny, normal development of hepatocytic cells, and the development of liver cancer. This work provides a resource for protein expression data for researchers studying human developmental hepatology, hematology, immunology, and liver sinusoidal endothelial cell biology. The authors have submitted a manuscript that further evaluates these data on hepatoblasts, LSECs, and hematopoietic cells .
Cells were analyzed using an LSR II flow cytometer (BD) (Table 1) and data analyzed using FlowJo software, version 9 (FlowJo, Inc.) for presentation . The number of events collected was maximized to provide the largest possible number of events for analysis and ranged from 3.33 × 104 to 2.80 × 105 events/sample, with a mean of 1.06 × 105 events. This sometimes resulted in drop-off in flow rate at the end of sample collection or a rapid increase owing to air bubbles. This can be mitigated using the time parameter to select for analysis only events collected from a period of stable flow rate (Fig. 1 A – all figures stored in the data repository listed in Table 1). Live cells, lacking propidium iodide staining (Fig. 1B), can be selected and further gated to reduce the number of doublet events (Fig. 1 C) and small debris (Fig. 1D) based on light scatter measurements to improve the quality of data for analysis.
As the fetal liver is primarily a hematopoietic organ, the cells were stained with a combination of CD45 and CD235a antibodies to identify blood cells (Fig. 1E). Before staining, the liver cells were depleted of erythrocytes by immunomagnetic bead depletion [2, 5] but as this depletion is not fully effective – specially with immature erythrocytes that express low levels of CD235a – additional staining with CD235a was performed to better differentiate the hematopoietic from the non-hematopoietic fraction. Cells stained with fluorochrome-labelled non-specific IgG1 antibodies can be used to guide the placement of gates as shown in Fig. 2 A-B.
Samples were stained with CD14 and CD326 to differentiate subpopulations of non-hematopoietic cells (Fig. 1 F). High levels of CD326 expression and low levels of CD14 expression can be used to identify fetal hepatoblasts (Fig. 1 F and 2 C) [2,3,4]. Liver sinusoidal endothelial cells (LSECs) can be gated based on high expression levels of CD14 . The different light scatter properties of hematopoietic cells (Fig. 1G), hepatoblasts (Fig. 1 H), and LSECs (Fig. 1I) further indicate differences in the size and complexities of these cells. Using the common markers stained in all the samples, it is possible to further identify other subpopulations of cells beyond those described.
Collection of data using the flow cytometer took place over 19.5 h: the first of four 96-well sample plates was completed in just over 3.5 h, followed by a break in analysis for 6.7 h, and then the 3 remaining plates were analyzed in succession over 3.5 h, 3.4 h, and 2.1 h for the partially filled fourth plate. Data analysis revealed some shifts in the frequencies of cell populations over the course of the experiment (Fig. 3). Notably, the hepatoblast population was negatively affected by the prolonged period of cold storage.
The complete list of antigens and antibody clones are indicated in Fig. 4. Analysis of the antigens in the context of CD45/CD235a expression is shown in Fig. 5. In Fig. 6, the expression of the antigens is shown on the non-hematopoietic cell fraction using polychromatic plots to indicate CD14 expression as shown in Fig. 1 J. The frequency of antigen expression as well as the mean fluorescence intensity of antigen expression for gated CD45/CD235a+ hematopoietic cells (Fig. 1E), CD326++CD14low hepatoblasts, and CD14++ LSECs (Fig. 1 F) are listed in Fig. 4.
The data are derived from a single midgestation specimen and, therefore, only represent a single moment in time during a dynamic period of ontogeny for the liver.
A single specimen cannot represent the diversity in phenotype that may result from genetic differences, sex differences, and environmental factors.
The long period to time required to collect the data had a detrimental influence on the hepatoblast population resulting in fewer CD326++CD14low events collected at the end of the experiment than at the beginning.
Availability of data and materials
The data described in this Data note can be freely and openly accessed on Flowrepository.org under “Human 21 week gestation liver phenotype (Repository ID: FR-FCM-Z58Z)” . Please see Table 1 for details and links to the data, methods, and analyses.
liver sinusoidal endothelial cells
Muench MO. Stem cells and progenitors in liver development. San Rafael: Morgan & Claypool Life Science Publishers; 2012. p. 126.
Fomin ME, Tai LK, Bárcena A, Muench MO. Coexpression of CD14 and CD326 discriminate hepatic precursors in the human fetal liver. Stem Cells Dev. 2011;20(7):1247–57.
Schmelzer E, Wauthier E, Reid LM. The phenotypes of pluripotent human hepatic progenitors. Stem Cells. 2006;24(8):1852–8.
Schmelzer E, Zhang L, Bruce A, Wauthier E, Ludlow J, Yao HL, et al. Human hepatic stem cells from fetal and postnatal donors. J Exp Med. 2007;204(8):1973–87.
Fomin ME, Zhou Y, Beyer AI, Publicover J, Baron JL, Muench MO. Production of factor VIII by human liver sinusoidal endothelial cells transplanted in immunodeficient uPA mice. PLoS ONE. 2013;8(10):e77255.
Muench MO, Fomin ME, Gutierrez AG, López-Terrada D, Gilfanova R, Nosworthy C, et al. CD203c is expressed by human fetal hepatoblasts and distinguishes subsets of hepatoblastoma. Frontiers in Oncology 2022;(In Press).
Muench MO. Human 21 week gestation liver phenotype. FlowRepository. 2022; Repository ID: FR-FCM-Z58Z. Available from: https://flowrepository.org/id/FR-FCM-Z58Z.
We thank Dr. Renata Gilfanova for proofreading the manuscript.
This work was supported by the California Institute for Regenerative Medicine, grant number DISC1-08855. C.N. was supported by a Bridges to Stem Cell Research and Therapy Award EDUC2-08400 from the California Institute of Regenerative Medicine. The content is solely the responsibility of the authors and does not necessarily represent the official views of the California Institute for Regenerative Medicine or any other agency of the State of California.
Ethics approval and consent to participate
A human 21 weeks’ gestation liver, of unknown sex, was obtained with approval of the University of California at San Francisco Institutional Review Board (UCSF IRB #10–00768) in accordance with the Declaration of Helsinki. Written informed consent was obtained for the donation from a woman undergoing elective abortion at San Francisco General Hospital. After the decision to have an abortion was made, the donor was offered the possibility to donate tissue for research without any benefit to themselves. Consent was obtained by clinic staff, not the researchers involved in this study, to ensure anonymity of the donor. The donor had the choice to decline donation without the choice affecting their medical treatment.
Consent for publication
The authors declare that they have no competing interests.
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Muench, M.O., Nosworthy, C. Antibody screening data of human midgestation liver cells with a focus on hematopoietic, liver sinusoidal endothelial, and hepatoblast cell-populations. BMC Res Notes 15, 358 (2022). https://doi.org/10.1186/s13104-022-06229-3