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Ion transport regulation by P2Y receptors, protein kinase C and phosphatidylinositol 3-kinase within the semicircular canal duct epithelium
BMC Research Notes volume 3, Article number: 100 (2010)
The ionic composition of the luminal fluid in the vestibular labyrinth is maintained within tight limits by the many types of epithelial cells bounding the lumen. Regulatory mechanisms include systemic, paracrine and autocrine hormones along with their associated intracellular signal pathways. The epithelium lining the semicircular canal duct (SCCD) is a tissue that is known to absorb sodium and calcium and to secrete chloride.
Transport function was assessed by measurements of short circuit current (I sc ) and gene transcript expression was evaluated by microarray. Neither ATP nor UTP (100 microM) on the apical side of the epithelium had any effect on I sc . By contrast, basolateral ATP transiently increased I sc and transepithelial resistance dropped significantly after basolateral ATP and UTP. P2Y2 was the sole UTP-sensitive purinergic receptor expressed. I sc was reduced by 42%, 50% and 63% after knockdown of α-ENaC, stimulation of PKC and inhibition of PI3-K, while the latter two increased the transepithelial resistance. PKCdelta, PKCgamma and PI3-K were found to be expressed.
These observations demonstrate that ion transport by the SCCD is regulated by P2Y2 purinergic receptors on the basolateral membrane that may respond to systemic or local agonists, such as ATP and/or UTP. The sodium absorption from endolymph mediated by ENaC in SCCD is regulated by signal pathways that include the kinases PKC and PI3-K. These three newly-identified regulatory components may prove to be valuable drug targets in the control of pathologic vestibular conditions involving dysfunction of transport homeostasis in the ear, such as Meniere's disease.
The transduction of linear and rotational acceleration into the sense of balance is driven and dependent on the ion composition of the luminal fluid, endolymph, of the vestibular labyrinth . We have shown that the semicircular canal duct (SCCD) epithelium contributes to the homeostasis of endolymph by absorption of Na+, secretion of Cl- and absorption of Ca2+[2–4]. These processes are known to be controlled by glucocorticoids, β-adrenergic agonists and vitamin D, respectively [2–4]. The present study augments previous investigations by reporting data that show the involvement of protein kinase C (PKC) and phosphatidylinositol 3-kinase (PI3-K) in the regulation of Na+ transport by the SCCD and the regulation of transport and/or epithelial barrier function by the P2Y2 purinergic receptor.
Na+ absorption is mediated by Na+ entry from endolymph into the SCCD cytosol via apical epithelial Na+ channels (ENaC) and removal of Na+ from the cytosol across the basolateral membrane by the "Na+-pump", Na+, K+-ATPase . The K+ brought into the cell across the basolateral membrane by the Na+-pump recirculates passively through basolateral K+ channels . The classical ENaC is highly selective for Na+ and is composed of α, β and γ subunits . It has been found in mammals that expression of the three ENaC subunits undergo a tissue-specific non-coordinated regulation depending on the physiological context and this can occur at both the mRNA and protein levels . In the current study, we have tested the specific involvement of α-ENaC in Na+ absorption by SCCD by knockdown of gene expression with RNA interference.
The level of ENaC activity is regulated by a large number of factors, including complex signal pathways, such as protein kinase C (PKC) and phosphatidylinositol 3-kinase (PI3-K) signal pathways, which control the number of channels residing in the plasma membrane . Protein turnover in the membrane is relatively rapid and signals controlling the insertion and removal of channels exerts powerful control over the rate of Na+ absorption. The fractional open time of the channels is also regulated by PKC signaling pathways and phosphoinositide signal pathways that include activation of PI3-K [8–11]. The putative participation of PKC and phosphoinositide signaling in SCCD Na+ absorption is tested in the current investigation by electrophysiology and gene array.
Purinergic signaling via receptors of the P2Y family constitutes an additional pathway that is known to regulate ENaC-mediated Na+ absorption and Cl- transport in other epithelia [12–15]. Experiments are described here that demonstrate the involvement of P2Y receptors in regulating transepithelial transport properties of SCCD, determine the location of the receptors and identify the candidate isoforms.
Results and Discussion
Primary cultures of rat SCCD were tested for 1) regulation of ion transport by purinergic signaling, 2) participation of α-ENaC subunits in Na+ absorption, 3) regulation of Na+ absorption by a phosphoinositide signal pathway and 4) by a protein kinase C pathway.
Array analysis of regulatory genes
Transcript analysis of genes investigated in this study are shown in Table S1 [Additional file 1]. Of the three UTP-sensitive receptors, only P2Y2 was present while P2Y4 and P2Y6 were absent and only P2Y2 was affected by dexamethasone treatment (known to regulate ENaC-mediated Na+ transport) with a small decrease in expression. Expression results for the ionotropic P2X receptors were not consistent across all probe sets. There were probes for six isoforms of protein kinase C (PKC), and only PKCdelta and PKCgamma were present and there were no significant changes of expression in the presence of dexamethasone [Additional file 1]. PI3-K was found to be present and there was no significant change of expression with dexamethasone.
Purinergic regulation of ion transport
Many epithelia express one or more purinergic receptors that are inserted into the apical and/or basolateral cell membranes and anion or cation transport is often increased or decreased by activation of these receptors [16–21]. The P2 receptor agonists ATP and UTP had no effects on the transepithelial voltage (VT), resistance (RT) and equivalent short-circuit current (Isc) at the apical membrane, but they both exerted complex control from the basolateral side (Figure 1a; Table S2) [Additional file 2].
Basolateral ATP (100 μM) caused biphasic changes in 3 out of 4 epithelia (Figure 1a). Initially, there were statistically significant peak increases in VT. The RT did not change over this short interval (about 30 - 120 s after addition of ATP). The resistance dropped significantly over the next ~60 s in all epithelia (n = 4). That drop in resistance was accompanied by a decrease in VT from the peak, while I sc did not change, suggesting that the drop in VT was caused by the drop in RT.
Basolateral UTP (100 μM) yielded similar results with a full bi-phasic response of VT (Figure 1b) in one of three epithelia and all three epithelia exhibited the sustained decrease in VT and RT (Figure 1c; Table S2) [Additional file 2]. The resistance dropped significantly over ~2 min in all three epithelia. That drop in resistance was accompanied by a decrease of VT but no change in I sc , suggesting that the drop in VT was caused by the drop in RT, as for basolateral ATP.
ATP can activate most ionotropic P2X and P2Y family receptors. However, UTP is potent at only 3 of the P2Y receptors (P2Y2, P2Y4 and P2Y6) and none of the P2X receptors . Our observations of transient stimulation of I sc and sustained decrease of RT along with the gene array results, suggest a dual signal pathway from basolateral P2Y2 receptors; one pathway controlling transepithelial ion transport and the other controlling the paracellular pathway. The results are in line with observations of UTP-stimulated Cl- secretion in endometrial gland epithelial cells , but do not rule out a transient stimulation of Na+ absorption. UTP-activated P2Y receptors have been shown to control paracellular tight junction pathways by decreasing the conductance in rabbit airway epithelium, opposite to the effect found here .
Effect of α-ENaC siRNA on dexamethasone stimulated Na+ absorption
Dexamethasone upregulates the expression levels of α-ENaC transcript and total protein in SCCD . This result is consistent with α-ENaC being a limiting constituent that acts as a chaperone for the other subunits of ENaC in trafficking to the apical membrane . We found (Figure 2) that α-ENaC siRNA (Table S3) [Additional file 3] significantly reduced the amiloride-sensitive I sc by 42% after incubation with dexamethasone (100 nM for 24 hr), whereas transfection with the non-silencing control siRNA (Table S3) [Additional file 3] changed the amiloride-sensitive I sc insignificantly (-2.5%). The specificity of the result is validated by the functional controls (transfection agent and non-silencing control siRNA) employed in the measurements of I sc . Amiloride is commonly used as a tool to block epithelial Na+ transport mediated by ENaC .
Effect of PKC activation and PI3-K inhibition on dexamethasone-stimulated Na+ absorption
SCCD were incubated with dexamethasone (100 nM for 24 hr) in the presence or absence of the PKC activator phorbol-12-myristate-13-acetate (PMA; 100 nM, 24 h), its inactive analog 4-alpha-PDD (100 nM, 24 h) or the PI3-K inhibitors wortmannin (100 nM, 9 h) or LY294002 (20 μM, 9 h), or its inactive analog LY 303511 (20 μM, 9 h). Both PMA and the PI3-K inhibitors were used at concentrations that have been reported to alter Na+ transport in other epithelia [9, 11, 25, 26].
Long-term exposure to PMA, but not 4-alpha-PDD, significantly reduced I sc by 49.7% and increased RT by 28.3% (Table S4) [Additional file 4]. Similarly, LY 294002, but not LY 303511, significantly reduced I sc by 62.5% and increased RT by 179% (Table S4) [Additional file 4]. Further, Wortmannin also inhibited I sc by 21.8% (Table S4) [Additional file 4]. The effects of PMA and LY 294002 on dexamethasone stimulated I sc , and on RT are consistent with positive regulation by PI3-K and negative regulation by PKC of glucocorticoid-stimulated Na+ absorption by SCCD epithelium (Figure 3).
Both PKC and PI3-K signaling pathways regulate corticosteroid-stimulated Na+ transport in several epithelial cells and expression systems via acute regulation of the open probability and rapid insertion and removal of ENaC in the cell membrane [8–11] and also the long-term expression level of ENaC in the apical membrane via regulation of ubiquitin-mediated degradation [27, 28].
The present study augments previous reports of ion transport systems that are expressed and characterized in SCCD epithelium, including sodium absorption and chloride secretion. We showed here that sodium absorption from endolymph mediated by ENaC in SCCD is regulated by signal pathways that include the kinases PKC and PI3-K (Figure 3). Further, purinergic receptors control both active ion transport and the integrity of the epithelial barrier. These kinases and the purinergic receptor(s) may prove to be valuable drug targets in the control of pathologic vestibular conditions such as Meniere's disease that involve dysfunction of transport homeostasis in the ear.
Materials and methods
Primary cultures of SCCD epithelium
Primary cultures from the semicircular canals of neonatal (3-5 days) Wistar rats were grown on 6.5 mm diameter Transwell permeable supports as described previously [2, 3, 23] (see Table S5 in Additional File 5 "Additional methods" for additional details of methods).
Amiloride (#A-7410, Sigma), Phorbol-12-myristate-13-acetate (PMA; #524400, Calbiochem), 4-alpha-phorbol-12, 13-didecanoate (4 alpha-PDD; #524394, Calbiochem), 2-(4-Morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY 294002; #L-9908, Sigma) and wortmannin (#W1628, Sigma) were dissolved in DMSO. Cyclodextrin-encapsulated dexamethasone (#D-2915, Sigma), 2-piperazinyl-8-phenyl-4H-1-benzopyran-4-one (LY 303511; #L-2786, Sigma) were dissolved in water. The purinergic agonist UTP (#U6625, Sigma) was dissolved directly in the physiological saline (below).
Epithelia were bathed in symmetrical bicarbonate-buffered physiological saline and the transepithelial voltage (VT), resistance (RT) and equivalent short-circuit current (Isc) were measured as before  (see additional File 5). Amiloride-sensitive I sc (AMIL - I sc ), which corresponds to the net transepithelial Na+ absorption, was measured for each condition studied. Each experimental series was performed on paired cultures from a single batch of dissociated canals.
Rat α-ENaC siRNA (four sets of sequences given in Table S1 [Additional file 1; Additional file 3] ) were designed by Qiagen company based on an available rat α-ENaC cDNA nucleotide sequence (GenBank Accession NM_031548). Both α-ENaC and non-silencing fluorescein-labeled control siRNA (#1022079, Qiagen) were reconstituted in siRNA Suspension Buffer (#301699, Qiagen).
Analysis of electrophysiologic data
Data are presented as mean values ± SEM from n observations. Data in Figure 1 were smoothed with a 15-point FFT smoothing algorithm in Origin software (OriginLab, Northampton, MA). An expanded, unfiltered pulse is shown in the inset to Figure 1a. Student's t-test was used to determine statistical significance of paired (ATP and UTP experiments) and unpaired samples. Differences were considered significant for P < 0.05.
Gene array analysis
Total RNA was extracted from control (n = 4) and dexamethasone-treated (n = 4) samples of SCCD primary cultures using RNeasy Micro Kit. The quality and quantity were determined as described previously . Affymetrix microarrays (see additional File 5 for details) were used to examine the expression of the genes investigated here by electrophysiology. Our methodology conforms to the MIAME (Minimum Information about a Microarray Experiment) guidelines and details are deposited with the data in [GEO: GSE6197].
Statistical algorithms [detection, change call, signal log ratio (SLR)] were used to identify differential gene expression in control and experimental samples. Probe sets were assigned as either present (P), absent (A), or marginal (M) based on detection p values (P, p < 0.05; M, p = 0.05-0.065; A, p > 0.065). Fold change was calculated from the median of the SLRs and only considered physiologically significant when the fold change was ≥ 1.3.
Elsevier, Marcus DC, Wangemann P: Cochlear and Vestibular Function and Dysfunction. Physiology and Pathology of Chloride Transporters and Channels in the Nervous System--From molecules to diseases. Edited by: Alvarez-Leefmans FJ, Delpire E. 2009, Elsevier, 421-433.
Pondugula SR, Sanneman JD, Wangemann P, Milhaud PG, Marcus DC: Glucocorticoids stimulate cation absorption by semicircular canal duct epithelium via epithelial sodium channel. Am J Physiol Renal Physiol. 2004, 286: F1127-F1135. 10.1152/ajprenal.00387.2003.
Milhaud PG, Pondugula SR, Lee JH, Herzog M, Lehouelleur J, Wangemann P, Sans A, Marcus DC: Chloride secretion by semicircular canal duct epithelium is stimulated via β2-adrenergic receptors. Am J Physiol, Cell Physiol. 2002, 283: C1752-C1760.
Nakaya K, Harbidge DG, Wangemann P, Schultz BD, Green E, Wall SM, Marcus DC: Lack of pendrin HCO3- transport elevates vestibular endolymphatic [Ca2+] by inhibition of acid-sensitive TRPV5 and TRPV6 channels. Am J Physiol Renal Physiol. 2007, 292: F1314-F1321. 10.1152/ajprenal.00432.2006.
Kosari F, Sheng S, Li J, Mak DO, Foskett JK, Kleyman TR: Subunit stoichiometry of the epithelial sodium channel. J Biol Chem. 1998, 273: 13469-13474. 10.1074/jbc.273.22.13469.
Weisz OA, Johnson JP: Noncoordinate regulation of ENaC: paradigm lost?. Am J Physiol Renal Physiol. 2003, 285: F833-F842.
Butterworth MB, Edinger RS, Frizzell RA, Johnson JP: Regulation of the epithelial sodium channel by membrane trafficking. Am J Physiol Renal Physiol. 2009, 296: F10-F24. 10.1152/ajprenal.90248.2008.
Booth RE, Stockand JD: Targeted degradation of ENaC in response to PKC activation of the ERK1/2 cascade. Am J Physiol Renal Physiol. 2003, 284: F938-F947.
Helms MN, Liu L, Liang YY, Al Khalili OK, Vandewalle A, Saxena S, Eaton DC, Ma HP: Phosphatidylinositol-3,4,5-trisphosphate (PIP3) mediates aldosterone stimulation of epithelial sodium channel (ENAC) and interacts with gamma-ENAC. J Biol Chem. 2005
Pochynyuk O, Bugaj V, Stockand JD: Physiologic regulation of the epithelial sodium channel by phosphatidylinositides. Curr Opin Nephrol Hypertens. 2008, 17: 533-540. 10.1097/MNH.0b013e328308fff3.
Yamagata T, Yamagata Y, Masse C, Tessier MC, Brochiero E, Dagenais A, Berthiaume Y: Modulation of Na+ transport and epithelial sodium channel expression by protein kinase C in rat alveolar epithelial cells. Can J Physiol Pharmacol. 2005, 83: 977-987. 10.1139/y05-071.
Roman RM, Feranchak AP, Salter KD, Wang Y, Fitz JG: Endogenous ATP release regulates Cl- secretion in cultured human and rat biliary epithelial cells. Am J Physiol Gastrointest Liver Physiol. 1999, 276: G1391-G1400.
Kunzelmann K, Schreiber R, Cook D: Mechanisms for the inhibition of amiloride-sensitive Na+ absorption by extracellular nucleotides in mouse trachea. Pflugers Arch. 2002, 444: 220-226. 10.1007/s00424-002-0796-y.
Palmer-Densmore M, Deachapunya C, Kannan M, O'Grady SM: UTP-dependent Inhibition of Na+ Absorption Requires Activation of PKC in Endometrial Epithelial Cells. J Gen Physiol. 2002, 120: 897-906. 10.1085/jgp.20028608.
Palmer ML, Lee SY, Carlson D, Fahrenkrug S, O'Grady SM: Stable knockdown of CFTR establishes a role for the channel in P2Y receptor-stimulated anion secretion. J Cell Physiol. 2006, 206: 759-770. 10.1002/jcp.20519.
Lee JH, Marcus DC: Purinergic signaling in the inner ear. Hear Res. 2008, 235: 1-7. 10.1016/j.heares.2007.09.006.
O'Mullane LM, Cook DI, Dinudom A: Purinergic regulation of the epithelial Na+ channel. Clin Exp Pharmacol Physiol. 2009, 36: 1016-1022. 10.1111/j.1440-1681.2009.05256.x.
Morse DM, Smullen JL, Davis CW: Differential effects of UTP, ATP, and adenosine on ciliary activity of human nasal epithelial cells. Am J Physiol, Cell Physiol. 2001, 280: C1485-C1497.
Paradiso AM, Ribeiro CM, Boucher RC: Polarized signaling via purinoceptors in normal and cystic fibrosis airway epithelia. J Gen Physiol. 2001, 117: 53-67. 10.1085/jgp.117.1.53.
Wilson SM, Law VW, Pediani JD, Allen EA, Wilson G, Khan ZE, Ko WH: Nucleotide-evoked calcium signals and anion secretion in equine cultured epithelia that express apical P2Y2 receptors and pyrimidine nucleotide receptors. Br J Pharmacol. 1998, 124: 832-838. 10.1038/sj.bjp.0701888.
Hwang TH, Schwiebert EM, Guggino WB: Extracellular ATP stimulates chloride secretion through different purinergic receptor subtypes on apical and basolateral membranes of rat tracheal epithelium [abstract]. J Gen Physiol. 1994, 104: 41a-
Poulsen AN, Klausen TL, Pedersen PS, Willumsen NJ, Frederiksen O: Nucleotide regulation of paracellular Cl- permeability in natural rabbit airway epithelium. Pflugers Arch. 2006, 452: 188-198. 10.1007/s00424-005-0023-8.
Pondugula SR, Raveendran NN, Ergonul Z, Deng Y, Chen J, Sanneman JD, Palmer LG, Marcus DC: Glucocorticoid Regulation of Genes in the Amiloride-Sensitive Sodium Transport Pathway by Semicircular Canal Duct Epithelium of Neonatal Rat. Physiol Genomics. 2006, 24: 114-123.
May A, Puoti A, Gaeggeler HP, Horisberger JD, Rossier BC: Early effect of aldosterone on the rate of synthesis of the epithelial sodium channel alpha subunit in A6 renal cells. J Am Soc Nephrol. 1997, 8: 1813-1822.
Wang J, Barbry P, Maiyar AC, Rozansky DJ, Bhargava A, Leong M, Firestone GL, Pearce D: SGK integrates insulin and mineralocorticoid regulation of epithelial sodium transport. Am J Physiol Renal Physiol. 2001, 280: F303-F313.
Blazer-Yost BL, Paunescu TG, Helman SI, Lee KD, Vlahos CJ: Phosphoinositide 3-kinase is required for aldosterone-regulated sodium reabsorption. Am J Physiol. 1999, 277: C531-C536.
Snyder PM, Olson DR, Thomas BC: Serum and Glucocorticoid-regulated Kinase Modulates Nedd4-2-mediated Inhibition of the Epithelial Na+ Channel. J Biol Chem. 2002, 277: 5-8. 10.1074/jbc.C100623200.
Debonneville C, Flores SY, Kamynina E, Plant PJ, Tauxe C, Thomas MA, Munster C, Chraibi A, Pratt JH, Horisberger JD, Pearce D, Loffing J, Staub O: Phosphorylation of Nedd4-2 by Sgk1 regulates epithelial Na+ channel cell surface expression. EMBO J. 2001, 20: 7052-7059. 10.1093/emboj/20.24.7052.
Marcus DC, Pondugula SR, Lee JH, Chiba T, Yamauchi D, Sanneman JD, Harbidge DG, Kampalli SB, Wu T, Wangemann P: Meniere's Disease & Inner Ear Homeostasis Disorders. Proceedings of the Fifth International Symposium. Ion transport pathways in the cochlea and vestibular labyrinth. 2006, 31-35.
We thank Donald Harbidge, Joel Sanneman and Bambi Harlow for technical assistance. This work was supported by NIH grants R01-DC00212 and P20-RR017686 to DCM.
The authors declare that they have no competing interests.
SRP carried out the study, including microdissection of tissues, primary cell cultures, quantitative analyses and contributed to writing the manuscript. NNR carried out the gene array analyses. DCM conceived of the study, participated in its design and coordination and contributed to writing the manuscript. Both authors read and approved the final manuscript.
Electronic supplementary material
Additional file 1:Table S1. Gene array of rat SCCD. Presence call and fold-change of transcripts for purinergic receptors, protein kinase C and phosphatidylinositol-3 kinase in SCCD epithelium. (PDF 12 KB)
Additional file 2:Table S2. Effects of ATP & UTP on SCCD transport. Effects of purinergic agonists on electrophysiology of ion transport. (PDF 15 KB)
Additional file 3:Table S3. siRNA for alpha-ENaC. Rat α-ENaC siRNA sequences used to knock down the functional expression of the α subunit of ENaC. (PDF 13 KB)
Additional file 4:Table S4. PKC and PI3-K regulation of Isc. Effects of PKC activator and PI3-K inhibitors on Isc and RT. (PDF 12 KB)
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Pondugula, S.R., Raveendran, N.N. & Marcus, D.C. Ion transport regulation by P2Y receptors, protein kinase C and phosphatidylinositol 3-kinase within the semicircular canal duct epithelium. BMC Res Notes 3, 100 (2010). https://doi.org/10.1186/1756-0500-3-100
- Purinergic Receptor
- Purinergic Signaling
- Transepithelial Voltage
- P2Y2 Purinergic Receptor