Lipid profiles of female and male Drosophila
© Oliver et al; licensee BioMed Central Ltd. 2011
Received: 31 January 2011
Accepted: 15 June 2011
Published: 15 June 2011
D. melanogaster is increasingly used as a lipid metabolism model, but the D. melanogaster metabolome is not well studied. A number of studies strongly suggest that lipid metabolism is linked to sexual behavior and gametogenesis.
We determined the levels of 400 different lipids in the non-gonadal soma of D. melanogaster females and males. We found higher levels of saturated cholesterol esters and lysophosphatidylcholine in males, and higher levels of polyunsaturated cholesterol esters in females. We also determined the levels of these lipids in females and males without a germline to determine if the absence of gamete "sinks" for metabolic products, such as yolk and lipid deposits in eggs, altered somatic lipid profiles. We observed little change in lipid profiles between these samples.
Overall lipid compositions are similar between the sexes, although there are differences in saturation states of two lipid classes, where saturated fatty acids were male-biased and polyunsaturated fatty acids were female-biased. The presence of a germline did not significantly influence lipid profiles, raising the possibility that germline-dependent changes in metabolic gene expression patterns serve a homeostatic purpose.
Lipids are the major energy storage molecules in cells and act as ligands in cell-cell and organism-organism pheromone signaling. Drosophila is an emerging model for studying all of these aspects of lipid biology [1–4]. We are particularly interested in sex differentiation and there is much indirect evidence that energy storage, cell-cell signaling, and pheromone lipid requirements differ between the Drosophila sexes.
The energy storage needs of females are higher than those of males due to egg production. Eggs, which are comprised primarily of lipoprotein particles (yolk) to store energy for embryonic development, make up a large fraction of the female's body mass and are therefore a metabolically expensive energy sink [5, 6]. The lipid signaling molecule ecdysone, best known for the role it plays in metamorphosis , is highly female-biased in adults  and plays a major role in production of yolk constituents in the ovarian somatic follicle cells and distantly located fatbody where they are transported to growing oocytes via the hemolymph [5, 9–11]. Metabolic enzymes such as the digestive chymotrypsins also show sex-biased expression in Drosophila[8, 12], again supporting the idea of a link between reproduction and energy homeostasis.
In addition to the direct connections between egg and lipid production, a number of lipids act as sex-biased hormones or pheromones that modulate pre- and post-mating behaviors in flies [13, 14]. These lipids might play a regulatory role in linking energy storage and reproduction. For example, the head fatbody shows sex-biased and/or circadian expression of a host of genes that encode lipid-binding proteins, some of which regulate feeding behavior, mating, or both [15–19]. Interestingly, the gene encoding the critical transcriptional regulator of most aspects of somatic sex differentiation, Doublesex, is expressed in a tightly regulated and spatially restricted set of cells in the nervous system, the fatbody, and a segment of the midgut where it is well positioned to modulate lipid metabolism in the full spectrum of cell types that might regulate a physiological axis including the brain, fatbody, and digestive tract of the sexes [20, 21]. Fruitless, another transcription factor controlling mating behavior is expressed in a limited set of neurons in Drosophila, and also regulates lipid storage . These studies suggest that the sex determination hierarchy is a regulator of energy homeostasis.
Such physiological relationships are perhaps best observed in fitness trade-off experiments that explore the competing optimal conditions for somatic and germline development. For example, reproduction reduces the lifespan of C. elegans and alters lipid metabolism [24, 25]. In Drosophila, increased egg production results in starvation sensitivity, and conversely, blocking egg maturation prevents a metabolic shift in the acid/base balance in the female gut at the onset of young adult female reproductive activity [26, 27]. These and other studies suggest that lifespan, reproduction, and energy metabolism are linked in both Drosophila and C. elegans. We have previously reported germline-dependent changes in the expression of genes encoding metabolic functions and suggested that they may underlie some of these metabolic/reproductive phenotypes . To support future work on lipid metabolism as it relates to sex, we undertook a broad survey of lipid profiles in adult non-gonadal tissues. We also explored the possible influence of the germline on these profiles.
To obtain a reasonably comprehensive profile of lipids in the Drosophila soma, we examined 10 lipid classes: cardiolipin, cholesterol ester, diacylglycerol, free fatty acid, lysophosphatidylcholine, phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin, and triacylglycerol by mass spectrometry (Lipomics Technologies, Sacramento CA). We made lipid determinations on mated sexed adult flies of the genotype tud 1 bw 1 sp 1 /CyO at 5-7 days after eclosion. To eliminate direct germline contributions to the lipid profiles, we removed the gonads prior to extraction. This also results in the loss of hemolymph and therefore most of the circulating lipids. To determine if lipid profiles differed due to indirect effects of the germline on somatic physiology, we examined flies from homozgyous tud 1 mothers. The progeny of homozygous tud 1 mothers do not form a germline, while progeny of heterozygous tud 1 mothers have a fully functional germline. This allowed us to examine the effect of the germline on somas with the same zygotic genotype. This is one of the same maternal/zygotic genotypes we previously described for expression profiling . Flies were grown on a standard rich cornmeal/sugar/yeast/agar media (<https://stockcenter.ucsd.edu/info/food_cornmeal.php>, Drosophila Species Stock Center, Tucson AZ); at 22°C; with 60% relative humidity; under constant light. We obtained lipid profiles from 8 samples, 4 from each sex, further stratified by germline status (Additional File 1). Note that statistical power was strongest for overall lipid profiles in adult flies where sample size was 8 and weakest for germline status within sex where sample size was 2. Because of the limited differences in lipid levels observed, collapsing germline classes to increase power was statistically justified by homogeneity.
We then binned lipid classes into saturated, monounsaturated, and polyunsaturated fatty acids. Again, we observed no significant differences between the flies with or without a germline within each sex, but we did observe sex-bias in the saturation states of cholesterol esters and lysophosphatidylcholines (Figure 2b-d). Since we observed no significant differences due to germline status (p > 0.05, t-test), we treated these within-sex samples as an additional level of replication in order to increase the power of statistical tests for the differences in lipid saturation between sexes. As suggested by the initial exploratory analysis, we observed significantly higher saturated cholesterol ester and lysophosphatidylcholine levels in males (p < 0.005, t-test) and an increase in polyunsaturated and/or monounsaturated cholesterol ester and lysophosphatidylcholine levels in females (p < 0.05, t- test). Given that lecithin:cholesterol acyltransferase transfers fatty acids from phosphatidylcholine to form cholesterol ester and lysophosphatidylcholine, these differences in saturation states may be linked.
Our a priori hypothesis was that lipid profiles would differ dramatically between sexes and especially between flies with or without a germline. We provide no evidence to support the hypothesis that lipid profiles in the non-gonadal soma are germline-dependent. However, we did observe sex-biased saturation states. It is intriguing that the saturation differences we observed were in the lysophosphatidylcholine and cholesterol ester classes, as lysophosphatidylcholine and cholesterol ester are produced by LCAT, an enzyme implicated in Low and High Density Lipoprotein particle formation . Drosophila egg development relies on Low Density Lipoprotein particles that are taken-up from the hemolymph , which is also intriguing. But in the absence of eggs, we would have expected some change in the lysophosphatidylcholine or cholesterol ester profiles in the female soma. Thus the germline-dependent expression of genes encoding various lipid metabolism enzymes [8, 27] is not mirrored by germline-dependent lipid profiles. One hypothesis is that those changes in gene expression maintain lipid homeostasis in the absence of a germline "sink" for lipids.
Saturation states have been implicated in mating behavior in flies. The sex-specific enzyme 1 (sxe1) locus encodes a putative fatty acid hydrolase required for high mating efficiency. In the absence of sxe1 the saturation states of multiple lipids are altered in male heads suggesting that lipid saturation plays a role in mating behavior . The lipid desaturase 1 locus (dsat1) is required for both pheromone signaling and the starvation response in flies [30–32]. Our work suggests that the major lipid differences between the sexes are restricted to saturation states. Saturation states may be an area of further investigation for those interesting in tying together the emerging physiological axis that coordinates mating and feeding behavior with energy storage and gametogenesis.
This research was supported by the Intramural Research Program of the NIH, National Institute of Diabetes and Digestive and Kidney Diseases.
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