Array data extractor (ADE): a LabVIEW program to extract and merge gene array data
© Kurtenbach et al.; licensee BioMed Central Ltd. 2013
Received: 25 July 2013
Accepted: 26 November 2013
Published: 1 December 2013
Large data sets from gene expression array studies are publicly available offering information highly valuable for research across many disciplines ranging from fundamental to clinical research. Highly advanced bioinformatics tools have been made available to researchers, but a demand for user-friendly software allowing researchers to quickly extract expression information for multiple genes from multiple studies persists.
Here, we present a user-friendly LabVIEW program to automatically extract gene expression data for a list of genes from multiple normalized microarray datasets. Functionality was tested for 288 class A G protein-coupled receptors (GPCRs) and expression data from 12 studies comparing normal and diseased human hearts. Results confirmed known regulation of a beta 1 adrenergic receptor and further indicate novel research targets.
Although existing software allows for complex data analyses, the LabVIEW based program presented here, “Array Data Extractor (ADE)”, provides users with a tool to retrieve meaningful information from multiple normalized gene expression datasets in a fast and easy way. Further, the graphical programming language used in LabVIEW allows applying changes to the program without the need of advanced programming knowledge.
KeywordsArray Data Extractor ADE Gene expression array Microarray data analysis Meta-analysis Combining of multiple datasets Statistics Merge p-values
High-throughput gene expression array technologies are commonly used in biomedical research and provide huge amounts of data. Today, there are close to one million preprocessed datasets publicly available repositories like the NCBI Gene Expression Omnibus , ArrayExpress  or the Stanford Microarray Database . This provides researchers with the opportunity to detect novel treatment targets for various diseases , discover and refine signaling pathways, and to identify unknown interaction networks. Combining and comparing data from different studies is a rewarding approach, but comparing data across several studies is a challenging task. Various approaches have been published to normalize and refine data to detect meaningful expression changes in genes/networks and there are several software packages, e.g. the open source software Bioconductor , allowing for complex microarray analysis like pre-processing, quality assessment, differential expression, clustering and classification, and gene set enrichment analysis. Other examples of open source software are the TM4 Microarray Software Suite  and GenePattern . Whilst other software packages allow very advanced data processing, performing a meta-analysis with data from multiple studies and platforms is still difficult for a “bench” scientist, and there is a lack of user-friendly software allowing researchers do so in a fast and easy way. A remarkable online tool, INMEX , has recently been published, providing user-friendly web-based platform for meta-analysis, but other available tools require substantial bioinformatics skills perform cross-platform meta-analysis [8, 9]. Here, we present a LabVIEW program, Array Data Extractor (ADE), which allows users to extract expression information for a list of genes from multiple datasets, merge it into one output file, and perform basic statistics. Although e.g. INMEX can perform much more advanced meta-analysis, ADE allows working offline with large datasets, easy modification of the code (see below), and to prioritize and exclude array spots according to their specificity.
LabVIEW is a graphical programming language, where code is written by wiring together graphical modules. While LabVIEW contains the same concepts found in most traditional programming languages, such as different data types, loops, variables, and object-oriented programming, the visual representation allows for easy access and modification of the code, in contrast to programming languages where the code is written in text. It must be noted that the user needs a licensed copy of the basic LabVIEW software. The LabVIEW code can be compiled into an executable file if wished. LabVIEW was chosen as the programming platform, because (I) the graphical programming interface allows users without profound programming skills to edit the program, (II) many processing subroutines (statistics, data sorting, fitting) are built-in and can be applied to the program, (III) it is platform independent, and (IV) LabVIEW is an established software platform used for various research purposes in many laboratories. Program structures can be assigned to existing projects, which is why several LabVIEW programs for various purposes have been published [10–18].
Defined Groups will be clustered together correctly (because in some data files they are not) and a ratio of the means or medians (can be selected by the user on the interface) from the defined groups to the control group will be calculated. Because some studies provide their data non-logarithmic, the user can add “NOLOG” to the folder name of the respective study, which ADE will recognize, or turn on the automatic Log2 detection on the software interface. If automatic Log2 detection is turned on, ADE will display which studies it finds to be Log2 performed (please check if correct!).
In some gene expression arrays, multiple spots can be present for the same gene and there are several ways to deal with multiple probe sets . ADE can automatically prioritize or exclude samples by their name on the gene array: Affymetrix platforms usually provide information about spot specificity in the spot ID name (e.g. “_s_at”). By adding a list of extensions to the fourth column of the “Annotation.txt” file (see Additional file 1) users can define which samples shall be included and in which priority. If information about specificity is available but not included in the probes names (e.g. for some Illumina arrays), the user can add this information in the third row of the “Annotation.txt” file. If there are multiple spots for one gene with highest priority (or if no priorities are defined), ADE will select the spot with the highest mean/median value of the control group by default. Refined data will be placed in a “Refined Data” folder. ADE will not overwrite existing files.
ADE will merge the ratio values for each gene and study into a single spreadsheet. Further, it will insert the correct group names, the study number, and information about the sample sizes (N). ADE will not overwrite an existing file, but perform statistics on it if selected (see below).
If the “Make statistics?” button is activated, ADE will perform a statistical analysis of the data and provide a combined p-value for a gene being up- or down-regulated in all studies in the merged output file. As ADE will not overwrite existing files, it is possible to perform statistical analysis after an initial run, allowing the user to delete fractions (genes, studies, columns) or sort the merged output file before performing statistics (the first column, containing the gene names/IDs, and the column labels at the bottom of the file (for the remaining columns) must remain unchanged). ADE will first calculate p-values for each gene in the respective studies by performing a one-tailed Student’s t-test. To merge p-values, we included Stouffer’s Z-transform method  and Fischer’s method . For Stouffer’s Z-transform method, p-values are first transformed to Z scores, with Z i = Φ- 1(p1), Φ(⋅) being the standard normal cumulative distribution function (CDF). Z-Scores receive signs according to the gene being up- or down-regulated and summed to an overall Z-Score (Zs), with , N being the number of Z-scores. Z-scores are not weighted in this approach. Zs is finally transformed to a combined two-sided p-value (ps) with p s = 2Φ (Z s ). Fisher’s method uses , where is a chi-squared distribution with 2k degrees of freedom, k being the number of p-values. We found Stouffer’s method to be more restrictive, but other statistical approaches may also be considered [22–25].
Class A GPCRs significantly regulated in human heart disease
0.00E + 00
0.00E + 00
The analysis reported 43 class A GPCRs as differentially expressed in diseased human hearts with a p-value < 0.001 (Table 1), according to Stouffer’s Z-transform method. The confirmation of published experimental data demonstrates the functionality of the ADE software. For example, we found a highly significant down-regulation of the beta 1 adrenergic receptor (ADRB1) in 19/21 groups, which is well known for its crucial role in heart function and down-regulation in heart failure [36–38]. Other interesting candidates for in depth analysis were predicted, like P2Y receptor subunits, a receptor class expressed in various heart cells and regulating cardiovascular function in health and disease [39–46]. We also found a high significance for sphingosine-1-phospate receptor 3 (S1PR3) being down-regulated in 14/14 groups. Other S1PRs were differentially expressed with lower significances. S1PRs have multiple functions in the cardiovascular system including modulation of the heart rate, cardioprotection and vascular contraction [47–54]. A third interesting candidate among the ten most significantly regulated class A GPCRs is the prostaglandin E receptor 3 (PTGER3) in 21/22 groups. Activation of PTGER3 was shown to protect cardiomyocytes from oxidative stress  and reduce ischemia-induced arrhythmias and infarct size . Overexpression was shown to promote hypertrophy  and changes in EP3 receptor density were reported after occlusion of the left anterior descending coronary artery . These results exemplify how ADE can be utilized to quickly compare expression data as a starting point for further research. In summary, our analysis of diverse datasets from different heart disease groups strongly suggests that a substantial amount of class A GPCRs are significantly regulated.
We introduce an easy-to-use software tool to extract and analyze normalized expression data. This program provides researchers with a tool to analyze gene array data utilizing publicly available normalized expression data. Beyond this scope, far more sophisticated tools (cited above) may be used for more detailed analysis.
Be sure to use “.” as decimal separators.
Avoid duplicate gene names in “Genes of Interest. txt”.
If ADE reports that it can’t open a file, be sure the format of files is correct (.txt), and/or use sample files provided to test.
Moving Data folders between Mac and Windows systems may cause problems. Be sure to:
○ Delete the complete “Refined Data” and “Extracted Data” folders.
○ Check the Data Folder names, special characters may cause problems.
Availability and requirements
Project name: Array Data Extractor (ADE)
Project home page: Software and sample data is included in the supplement. Webpage will be designed upon publication.
Operating systems: Mac OS X, Windows, and Linux
Programming language: LabVIEW (National Instruments)
Other requirements: LabVIEW (National Instruments)
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