Y chromosome of the inbred mouse KK/Ta strain is associated with reduced body size in Y-consomic strains
© Suto; licensee BioMed Central Ltd. 2013
Received: 7 January 2013
Accepted: 11 February 2013
Published: 18 February 2013
We have established 17 Y chromosome consomic (Y-consomic) mouse strains in an inbred DH/Sgn strain. In this study, based on investigations in four different genetic backgrounds, we proved that the Y chromosome of the inbred mouse KK/Ta strain is associated with reduced body size.
In the DH-Chr Y-+/+ background, Y chromosome substitution significantly decreased the body weight in DH-Chr YKK-+/+ and DH-Chr YSJL-+/+ strains, and the DH-Chr YKK-+/+ strain was the lightest among the 17 Y-consomic strains. In the DH-Chr Y-Dh/+ background (Dh/+ mice have skeletal malformations and are usually lighter than +/+ mice), although Y chromosome substitution did not significantly alter the body weight, the DH-Chr YKK-Dh/+ strain was the lightest among the 17 Y-consomic-Dh/+ strains. In the (B6.Cg-A y × DH-Chr Y) F1-+/+ background, Y chromosome substitution significantly decreased the body weight and length in the (B6.Cg-A y × DH-Chr YKK) F1 hybrids. In the (B6.Cg-A y × DH-Chr Y) F1-A y /+ background (A y causes obesity and promotes linear growth), Y chromosome substitution significantly decreased body weight and length in the (B6.Cg-A y × DH-Chr YKK) F1-A y /+ hybrids.
A body-size-reducing effect of the Y chromosome of the KK/Ta mouse strain was observed irrespective of genetic background. The effect was observed in the presence of Dh and A y , the autosomal dominant mutations, both of which are known to have substantial effects on body size. These results suggest that there are Y-linked genes that control the body size in mice.
We have established 17 Y chromosome consomic (hereafter Y-consomic) mouse strains in an inbred DH/Sgn (hereafter DH) strain. There was a wide spectrum of variation in body weight and testis weight among the Y-consomic mouse strains [1, 2]. Thus, it was expected that there were Y-linked genes associated with body weight and testis weight. We identified several SNPs and gene polymorphisms that were associated with testis weight variation when the trait was evaluated as a quantitative trait . Although we have not yet identified SNPs and gene polymorphisms associated with body weight, we noted that the DH-Chr YKK strain was lighter than other Y-consomic strains . Therefore, we further investigated the effect of the Y chromosome by incorporating additional mice in this study. Based on the investigation in four different genetic backgrounds, we proved that the Y chromosome of the inbred mouse KK/Ta strain is associated with reduced body weight and length.
First, we analyzed Y-consomic strains in the DH strain background. Because DH includes both +/+ and Dh/+ genotypes at the dominant hemimelia (Dh) locus on chromosome 1, each Y-consomic strain includes both +/+ and Dh/+ mice. Some skeletal elements are lost in Dh/+ mice; therefore, Dh/+ mice are usually lighter than +/+ littermates (see Methods for details). We next analyzed Y-consomic strains with the Dh mutation. We further investigated the Y-consomic strains in combination with A y , the obesity mutation. The A y allele at the agouti locus on chromosome 2 is known to cause obesity and promote linear growth in mice (see Methods for details). When the males of each Y-consomic strain were crossed with females of the B6.Cg-A y strain, the F1 generation consisted of A y (yellow, A y /+) and non-A y (agouti, +/+) mice. We analyzed the F1-+/+ and F1-A y hybrids. This analysis allowed us to evaluate the effect of the Y chromosome in obese (F1A y ) animals as well as in addition to non-obese (F1 non-A y ) animals in the same genetic background. Thus, we investigated the effect of the Y chromosome in the presence of autosomal dominant mutations, both of which substantially affected body size.
The following Y-consomic strains were used in this study: DH-Chr YA (Y chromosome from A/J strain), DH-Chr YAKR (AKR/J), DH-Chr YB6 (C57BL/6J), DH-Chr YBALB (BALB/cA), DH-Chr YC3H (C3H/HeJ), DH-Chr YCAST (CAST/EiJ), DH-Chr YCBA (CBA/N), DH-Chr YCF1 (CF1/Sgn), DH-Chr YDBA (DBA/2J), DH-Chr YDDD (DDD/Sgn), DH-Chr YDH (identical to DH), DH-Chr YKK (KK/Ta), DH-Chr YRF (RF/J), DH-Chr YRR (RR/Sgn), DH-Chr YSJL (SJL/J), DH-Chr YSS (SS/Sgn), and DH-Chr YSWR (SWR/J). B6.Cg-A y strain was purchased from the Jackson Laboratory (Bar Harbor, ME, USA) and maintained at the National Institute of Agrobiological Sciences (NIAS, Tsukuba, Japan). Each Y-consomic strain included Dh/+ and +/+ mice with respect to the genotype at the Dh locus. Dh causes visceral and skeletal malformations of various degrees of severity [3, 4]. Visceral abnormalities include a small stomach, short intestine, hydropic kidneys, and congenital absence of the spleen. Skeletal malformations appear in the trunk caudally from the thorax, particularly in the hindlimbs. The abnormalities induced by Dh are expressed more severely in Dh/Dh than in Dh/+ animals. Because Dh/Dh mice die shortly after birth owing to their visceral abnormalities, only heterozygous Dh/+ mice were available for this study. The skeletal malformations in Dh/+ mice are worth mentioning. In Dh/+ mice, the number of lumbar vertebrae is reduced to five, as opposed to six in +/+ mice. Loss of the hallux (i.e., presence of only four digits) is commonly observed in Dh/+ mice. However, triphalangy of the hallux (i.e., presence of five digits with an extra phalange on the hallux) is also commonly observed. Polydactyly is sometimes observed and is associated with an additional phalange on the hallux (the number of metatarsal bones do not exceed five even in the case of polydactyly). Although the fibula is rarely affected, various lengths of the distal part of the tibia are frequently lost. Thus, Dh is associated essentially with reduction of skeletal elements. Dh/+ mice were distinguished from +/+ mice by the presence of hindlimb malformation, and the Dh/+ genotype was confirmed by the absence of the spleen on laparotomy. The Y-consomic strains in a DH background are hereafter designated as DH-Chr Y-+/+ and DH-Chr Y-Dh/+ for convenience.
Genetic backgrounds and numbers of mice in the Y-consomic strains used in this study
(♀B6.Cg-A y × ♂DH-Chr Y-+/+) F1-+/+ (F1-+/+)a
(♀B6.Cg-A y × ♂DH-Chr Y-+/+) F1-A y /+ (F1-A y /+)a
All mice were maintained in a specific-pathogen-free facility with a regular light cycle (12 h light and 12 h dark) and controlled temperature (23 ± 1°C) and humidity (50%). Food and water were freely available throughout the experimental period. DH-Chr Y-+/+ and DH-Chr Y-Dh/+ strains were fed a CE-2 (CLEA Japan Inc., Tokyo) and F1-+/+ and F1-A y /+ hybrids were fed a CRF-1 (Oriental Yeast Co. Ltd., Tokyo). We are uncertain whether or not the difference in the lot of diet might have any impacts on body weights and/or body sizes of mice. All animal experiments were performed in accordance with guidelines approved by the Institutional Animal Care and Use Committee of NIAS.
At the age of 80 days for DH-Chr Y-+/+ and DH-Chr Y-Dh/+ strains and at the age of 16 weeks for F1-+/+ and F1-A y /+ hybrids, the mice were weighed on an electric balance to the nearest 0.01 g after 4 h fasting. For F1-+/+ and F1-A y /+ hybrids, the anal–nasal length and tail length of each mouse were measured to the nearest 0.01 mm with digital calipers. Body length was defined as the anal–nasal length.
Normality of distribution of the trait data for each Y-consomic strain was tested by the Shapiro–Wilk W test (JMP 8, SAS Institute Inc., Cary, NC, USA). If the trait values did not follow a normal distribution, they were normalized using the Box–Cox transformation.
Statistical comparison of two groups was performed by the Student’s t-test. Effects of Y chromosome substitution were assessed using Dunnett’s multiple-comparison tests with the background DH strain as a reference. P <0.05 was considered statistically significant.
Body size is probably determined by multiple genes under the influence of non-genetic factors such as nutritional condition. To identify Y-linked gene polymorphisms associated with body size, it is essential to unify autosomal effects and to minimize non-genetic environmental influences. Thus, Y-consomic mouse strains are desirable and essential tools for investigating the effect of the Y chromosome on body size. There are several reports on the association of the Y chromosome with adult male height in humans, but the results are still contradictory [16, 17].
It is a fact that many reports on body size have been obtained in human studies . For example, the presence of gene associated with short stature in the pseudoautosomal region has been suggested in human [19, 20]. The gene SHOX (short stature homeobox) is now considered to be involved in idiopathic growth retardation and in the short-stature phenotype of patients with Turner syndrome [18, 21, 22]. The pseudoautosomal localization of SHOX suggested the presence of a Y-linked functional homolog, SHOXY. However, in mice, Shox is not pseudoautosomal but autosomal. Therefore, the effect of the Y chromosome on body size observed in this study should not be attributed to Shoxy. Thus, it was suggested that there are other genes on the Y chromosome that influence body size in mice. We have genotyped Y-linked SNPs and other gene polymorphisms in these Y-consomic strains . However, none of them showed polymorphisms specific to the KK/Ta strain clearly excluding these gene polymorphisms as candidates.
As a next step, it is crucial to determine at what age the body size of DH-Chr YKK strain becomes smaller than that of the other Y-consomic strains. Analysis of growth curves will be useful for this purpose. Because the difference was apparent at 80 days at the latest, the effect of the Y chromosome is expected to manifest earlier. The effect may already be apparent during the fetal period because the effect of Y chromosome on fetal growth rate has been hypothesized . Comparison of birth weights will be critical to test of this hypothesis.
A body-size-reducing effect of the Y chromosome of the KK/Ta mouse strain was observed irrespective of genetic background. The effect was observed in the presence of Dh and A y , the autosomal dominant mutations, both of which are known to have substantial effect on body size. These results suggest that there are Y-linked genes that control body size in mice.
This work was supported by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (nos. 15500305 and 19500373).
- Suto J: Genetic dissection of testis weight in a mouse strain having an extremely large testis: major testis weight determinants are autosomal rather than Y-linked on the basis of comprehensive analyses in Y-chromosome consomic strains. Proc Jpn Acad Ser B. 2008, 84: 393-406. 10.2183/pjab.84.393.View ArticleGoogle Scholar
- Suto J: Genetic dissection of testis weight in mice: quantitative trait locus analysis using F2 intercrosses between strains with extremely testis weight, and association study using Y-consomic strains. Mamm Genome. 2011, 22: 648-660. 10.1007/s00335-011-9353-3.PubMedView ArticleGoogle Scholar
- Searle AG: The genetics and morphology of two ‘luxoid’ mutants in the house mouse. Genet Res Camb. 1961, 5: 171-197.View ArticleGoogle Scholar
- Suto J, Wakayama T, Imamura K, Goto S, Fukuta K: Skeletal malformations caused by the Dh (Dominant hemimelia) gene in mice. Exp Anim. 1996, 45: 95-98. 10.1538/expanim.45.95.PubMedView ArticleGoogle Scholar
- Heston WE, Vlahakis G: Influence of the Ay gene on mammary-gland tumors, hepatomas, and normal growth in mice. J Natl Cancer Inst. 1961, 26: 969-983.PubMedGoogle Scholar
- Bultman SJ, Michaud EJ, Woychik RP: Molecular characterization of the mouse agouti locus. Cell. 1992, 71: 1195-1204. 10.1016/S0092-8674(05)80067-4.PubMedView ArticleGoogle Scholar
- Miller MW, Duhl DM, Vrieling H, Cordes SP, Ollmann MM, Winkes BM, Barsh GS: Cloning of the mouse agouti gene predicts a secreted protein ubiquitously expressed in mice carrying the lethal yellow mutation. Genes Dev. 1993, 7: 454-467. 10.1101/gad.7.3.454.PubMedView ArticleGoogle Scholar
- Robbins LS, Nadeau JH, Johnson KR, Kelly MA, Rosell-Rehfuss L, Baack E, Mountjoy KG, Cone RD: Pigmentation phenotypes of variant extension locus alleles result from point mutations that alter MSH receptor function. Cell. 1993, 26: 827-834.View ArticleGoogle Scholar
- Lu D, Willard D, Patel IR, Kadwell S, Overton L, Kost T, Luther M, Chen W, Woychik RP, Wilkinson WO, Cone RD: Agouti protein is an antagonist of the melanocyte-stimulating-hormone receptor. Nature. 1994, 371: 799-802. 10.1038/371799a0.PubMedView ArticleGoogle Scholar
- Duhl DM, Vrieling H, Miller KA, Wolff GL, Barsh GS: Neomorphic agouti mutations in obese yellow mice. Nat Genet. 1994, 8: 59-65. 10.1038/ng0994-59.PubMedView ArticleGoogle Scholar
- Michaud EJ, Bultman SJ, Stubbs LJ, Woychik RP: The embryonic lethality of homozygous lethal yellow mice (Ay/Ay) is associated with the disruption of a novel RNA-binding protein. Genes Dev. 1993, 7: 1203-1213. 10.1101/gad.7.7a.1203.PubMedView ArticleGoogle Scholar
- Michaud EJ, Bultman SJ, Klebig ML, van Vugt MJ, Stubbs LJ, Russell LB, Woychik RP: A molecular model for the genetic and phenotypic characteristics of the mouse lethal yellow (Ay) mutation. Proc Natl Acad Sci USA. 1994, 91: 2562-2566. 10.1073/pnas.91.7.2562.PubMedPubMed CentralView ArticleGoogle Scholar
- Chen AS, Marsh DJ, Trumbauer ME, Frazier EG, Guan XM, Yu H, Rosenblum CI, Vongs A, Feng Y, Cao L, Metzger JM, Strack AM, Camacho RE, Mellin TN, Nunes CN, Min W, Fisher J, Gopal-Truter S, Maclntyre DE, Chen HY, Van der Ploeg LH: Inactivation of the mouse melanocortin-3 receptor results in increased fat mass and reduced lean body mass. Nat Genet. 2000, 26: 97-102. 10.1038/79254.PubMedView ArticleGoogle Scholar
- Huszar D, Lynch CA, Fairchild-Huntress V, Dunmore JH, Fang Q, Berkemeier LR, Gu W, Kesterson RA, Boston BA, Cone RD, Smith FJ, Campfield LA, Burn P, Lee F: Targeted disruption of the melanocortin-4 receptor results in obesity in mice. Cell. 1997, 88: 131-141. 10.1016/S0092-8674(00)81865-6.PubMedView ArticleGoogle Scholar
- Ollmann MM, Wilson BD, Yang YK, Kerns JA, Chen Y, Gantz I, Barsh GS: Antagonism of central melanocortin receptors in vitro and in vivo by agouti-related protein. Science. 1997, 278: 135-138. 10.1126/science.278.5335.135.PubMedView ArticleGoogle Scholar
- Ellis JA, Stebbing M, Harrap SB: Significant population variation in adult male height associated with the Y chromosome and the aromatase gene. J Clin Endocrinol Metab. 2001, 86: 4147-4150. 10.1210/jc.86.9.4147.PubMedView ArticleGoogle Scholar
- Weedon MN, Turner M, Knight B, Clark P, Hattersley AT, Frayling TM: Variants in the aromatase gene and on the Y-chromosome are not associated with adult height or insulin resistance in a UK population. Clin Endocrinol. 2003, 59: 175-179. 10.1046/j.1365-2265.2003.01797.x.View ArticleGoogle Scholar
- Online Mendelian Inheritance in Man. [http://www.ncbi.nlm.nih.gov/omim]
- Ogata T, Matsuo N: Comparison of adult height between patients with XX and XY gonadal dysgenesis: support for a Y specific growth gene(s). J Med Genet. 1992, 29: 539-541. 10.1136/jmg.29.8.539.PubMedPubMed CentralView ArticleGoogle Scholar
- Ogata T, Matsuo N: The Y specific growth gene(s): how does it promote stature?. J Med Genet. 1997, 34: 323-325. 10.1136/jmg.34.4.323.PubMedPubMed CentralView ArticleGoogle Scholar
- Ellison JW, Wardak Z, Young MF, Robey PG, Webster M, Chiong W: PHOG, a candidate gene for involvement in the short stature of Turner syndrome. Hum Mol Genet. 1997, 6: 1341-1347. 10.1093/hmg/6.8.1341.PubMedView ArticleGoogle Scholar
- Rao E, Weiss B, Fukami M, Rump A, Niesler B, Mertz A, Muroya K, Binder G, Kirsch S, Winkelmann M, Nordsiek G, Heinrich U, Breuning MH, Ranke MB, Rosenthal A, Ogata T, Rappold GA: Pseudoautosomal deletions encompassing a novel homeobox gene cause growth failure in idiopathic short stature and Turner syndrome. Nat Genet. 1997, 16: 54-63. 10.1038/ng0597-54.PubMedView ArticleGoogle Scholar
- Ounsted C, Ounsted M: Effect of Y chromosome on fetal growth-rate. Lancet. 1970, 2: 857-858.PubMedView ArticleGoogle Scholar
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