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A de novo transcriptome assembly for the bath sponge Spongia officinalis, adjusting for microsymbionts

Abstract

Objectives

We report a transcriptome acquisition for the bath sponge Spongia officinalis, a non-model marine organism that hosts rich symbiotic microbial communities. To this end, a pipeline was developed to efficiently separate between bacterial expressed genes from those of eukaryotic origin. The transcriptome was produced to support the assessment of gene expression and, thus, the response of the sponge, to elevated temperatures, replicating conditions currently occurring in its native habitat.

Data description

We describe the assembled transcriptome along with the bioinformatic pipeline used to discriminate between signals of metazoan and prokaryotic origin. The pipeline involves standard read pre-processing steps and incorporates extra analyses to identify and filter prokaryotic reads out of the analysis. The proposed pipeline can be followed to overcome the technical RNASeq problems characteristic for symbiont-rich metazoan organisms with low or non-existent tissue differentiation, such as sponges and cnidarians. At the same time, it can be valuable towards the development of approaches for parallel transcriptomic studies of symbiotic communities and the host.

Objective

Sponges are organisms with simple body plan, lacking true tissue differentiation [1]. Moreover, they often host rich symbiotic bacterial communities, thus creating complex holobionts [2, 3]. These traits, combined with the diverse nature of the poriferan phylum and their vulnerability to global change makes them ideal case-study species (e.g. [4,5,6]). Although transcriptomic studies facilitated through NGS can provide sound answers to ecological questions, the lack of a reference genome makes the building a de novo assembly necessary, as for all non-model organisms. This becomes more challenging in sponges, as it is often difficult to discriminate between signals of metazoan and prokaryotic origin [7, 8], thus introducing biases to interpretation.

Here, we constructed the transcriptome of the Mediterranean bath sponge Spongia officinalis, an organism that has suffered a substantial decline in the past decades due to the combined impact of harvesting and mass mortalities attributed to extreme climatic events [9, 10]. The acquisition of the transcriptome was used to assess gene expression within a manipulative experiment, where individuals of the sponge were subjected to a gradient of elevated temperatures simulating extreme climatic events currently occurring during the warm season in its native habitats (see Table 1 data file 1 for experimental design). The results of the study are published in [4] and all data files are presented in Table 1.

Table 1 Overview of data files/data sets

The built transcriptome assembly comprises the only transcriptome reference available for S. officinalis and can serve as a baseline for further studies on the species. This transcriptome reference has already been used in studies of different focus (see [11]) indicating the importance of this transcriptome generation in various study fields. The proposed pipeline can be followed to overcome the technical RNASeq problems characteristic for symbiont-rich metazoan organisms with low or non-existent tissue differentiation, such as sponges and cnidarians.

Data description

Four S. officinalis individuals collected from natural populations from the island of Crete, Greece, were reared in closed tanks and experimentally exposed to elevated temperatures approximate an extreme climate event naturally occurring in the sponge’s habitat during summer. The 50 m3 rearing tanks contained natural seawater collected from a pristine open-sea area, with temperature and salinity adjusted to reflect typical local conditions for the time of year (24 °C and 39 ppt, respectively). Two experimental tanks were employed, one as control (24 °C) and one as treatment with increasing temperature (up to 30 °C). Five sampling points initiated after 5 days acclimatization in the tanks and over a span of 6 days, resulted in 20 samples. RNA was extracted with TRIZOL (TRIzol™ Reagent, Thermo Fisher Scientific, Cat. number 15596026) following the manufacturer’s protocol. The quality control of the RNA revealed a unique profile. Apart from the expected 28 s, 18 s ribosomal bands two extra bands, possibly of 23 s, 16 s characteristic of the microbial ribosomal RNA, appeared at the agarose gel, which reflected a remarkably large proportion of prokaryotes in the extracted RNA (data file 2). For the library preparation we used the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, Cat. Number 20020594) and followed the protocol of the manufacturer for sequencing using the shortest possible fragmentation time and applying 13 cycles instead of 15 in the amplification library PCR at the very last step of the protocol. In total, 20 RNA libraries were sequenced on an Illumina HiSeq 2000 platform. Τhe amount of prokaryotic RNA in our extraction urged us to implement extra steps for excluding the prokaryotic sequences from our dataset (data file 3).

Sequencing yielded on average 12,933,232 raw paired reads per library (data set 1). Raw reads were quality controlled using multiple software in a workflow described in [12] and run through bash scripts (data file 4 and 5). The used software included scythe (version 0.994 BETA; https://github.com/vs.buffalo/scythe), sickle (version 1.33; https://github.com/najoshi/sickle), prinseq (version 0.20.4; http://prinseq.sourceforge.net/) and trimmomatic version 0.32 [13]. The quality-controlled data were used to build an initial Trinity (v2.1.1) [14] assembly (data file 6). However, given that a great percentage of sponge transcriptome is comprised of bacterial sequences, we downloaded all bacterial sequences from NCBI (data file 7) and removed all reads (2.2 to 17.6% of the reads of each sample) that were successfully mapped on them using riboPicker (ribopicker-standalone-0.4.3 version; https://sourceforge.net/projects/ribopicker/files/standalone/; command ribopicker.pl -c 47 -i 75 -l 40 -z 3). Then, we built another assembly with the remaining reads (data file 8). The reconstructed transcripts were then used for a similarity search through NOBLAST [15] against the Swiss-Prot database (e-value: 1.0E−5). Transcripts that had as best hit prokaryotic sequences (17.1% of the assembly) were eliminated leading to the final assembly (data file 9). Their corresponding reads were eliminated from the bam files as well (data file 10) and were excluded from downstream analyses.

Limitations

The proposed pipeline eliminates effectively most prokaryotic sequences within the sequenced dataset, however, it does not filter out non-sponge eukaryotic sequences that are often present due to existence of symbiotic eukaryotes as well, e.g. fungi and dinoflagellates.

Availability of data materials

The data described in this Data note can be freely and openly accessed on figshare (https://doi.org/10.6084/m9.figshare.10001870.v3) and SRA (https://www.ncbi.nlm.nih.gov/sra/?term=SRP150632). Please see Table 1 and reference list for details and links to the data.

Abbreviations

RNASeq:

RNA-sequencing the use of next-generation sequencing to assess the presence and quantity of the expressed RNA in a biological sample

NGS:

next-generation sequencing

References

  1. 1.

    Leys SP, Hill A. The physiology and molecular biology of sponge tissues. Adv Mar Biol. 2012;62:1–56. https://doi.org/10.1016/b978-0-12-394283-8.00001-1.

    Article  PubMed  Google Scholar 

  2. 2.

    Thomas T, Moitinho-Silva L, Lurgi M, Björk JR, Easson C, Astudillo-García C, et al. Diversity, structure and convergent evolution of the global sponge microbiome. Nat Commun. 2016;7:11870. https://doi.org/10.1038/ncomms11870 (PMID: 27306690; PMCID: PMC4912640).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  3. 3.

    Moitinho-Silva L, Nielsen S, Amir A, Gonzalez A, Ackermann GL, Cerrano C. The sponge microbiome project. Gigascience. 2017;6(10):1–7. https://doi.org/10.1093/gigascience/gix077.

    Article  PubMed  Google Scholar 

  4. 4.

    Koutsouveli V, Manousaki T, Riesgo A, Lagnel J, Kollias S, Tsigenopoulos CS, Arvanitidis C, Dounas C, Magoulas A, Dailianis T. Gearing up for warmer times: gene expression of Spongia officinalis under heat-stress reveals recruited molecular mechanisms and potential for resilience. Front Mar Sci. 2019;6:786. https://doi.org/10.3389/fmars.2019.00786.

    Article  Google Scholar 

  5. 5.

    Conaco C, Neveu P, Zhou H, Arcila ML, Degnan SM, Degnan BM, et al. Transcriptome profiling of the demosponge Amphimedon queenslandica reveals genome-wide events that accompany major life cycle transitions. BMC Genomics. 2012. https://doi.org/10.1186/1471-2164-13-209.

    Article  PubMed  PubMed Central  Google Scholar 

  6. 6.

    Dailianis T, Tsigenopoulos CS, Dounas C, Voultsiadou E. Genetic diversity of the imperilled bath sponge Spongia officinalis Linnaeus, 1759 across the Mediterranean Sea: patterns of population differentiation and implications for taxonomy and conservation. Mol Ecol. 2011. https://doi.org/10.1111/j.1365-294x.2011.05222.x.

    Article  PubMed  Google Scholar 

  7. 7.

    Cebrian E, Uriz MJ, Garrabou J, Ballesteros E. Sponge mass mortalities in a warming Mediterranean Sea: are cyanobacteria-harboring species worse off? PLoS ONE. 2011. https://doi.org/10.1371/journal.pone.0020211.

    Article  PubMed  PubMed Central  Google Scholar 

  8. 8.

    Webster NS, Cobb RE, Negri AP. Temperature thresholds for bacterial symbiosis with a sponge. ISME J. 2008;2:830–42. https://doi.org/10.1038/ismej.2008.42.

    CAS  Article  PubMed  Google Scholar 

  9. 9.

    Gerovasileiou V, Dailianis T, Sini M, Otero Mar del M, Numa C, Katsanevakis S, et al. Assessing the regional conservation status of sponges (Porifera): the case of the Aegean ecoregion. Mediterr Mar Sci. 2018;19:526–37. https://doi.org/10.12681/mms.14461.

    Article  Google Scholar 

  10. 10.

    Webster NS. Sponge disease: a global threat? Environ Microbiol. 2007;9:1363–75. https://doi.org/10.1111/j.1462-2920.2007.01303.x.

    CAS  Article  PubMed  Google Scholar 

  11. 11.

    Plese B, Rossi ME, Kenny NJ, Taboada S, Koutsouveli V, Riesgo A. Trimitomics: an efficient pipeline for mitochondrial assembly from transcriptomic reads in nonmodel species. Mol Ecol Resour. 2019;19:1230–9. https://doi.org/10.1111/1755-0998.13033.

    CAS  Article  PubMed  Google Scholar 

  12. 12.

    Ilias A, Lagnel J, Kapantaidaki DE, Roditakis E, Tsigenopoulos CS, Vontas J, et al. Transcription analysis of neonicotinoid resistance in Mediterranean (MED) populations of B. tabaci reveal novel cytochrome P450s, but no nAChR mutations associated with the phenotype. BMC Genomics. 2015;16:939. https://doi.org/10.1186/s12864-015-2161-5.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  13. 13.

    Bolger AM, Lohse M, Usadel B. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics. 2014. https://doi.org/10.1093/bioinformatics/btu170.

    Article  PubMed  PubMed Central  Google Scholar 

  14. 14.

    Haas BJ, Papanicolaou A, Yassour M, Grabherr M, Blood PD, Bowden J, et al. De novo transcript sequence reconstruction from RNA-Seq: reference generation and analysis with Trinity. Nat Protoc. 2013. https://doi.org/10.1038/nprot.2013.084.

    Article  PubMed  PubMed Central  Google Scholar 

  15. 15.

    Lagnel J, Tsigenopoulos CS, Iliopoulos I. NOBLAST and JAMBLAST: new options for BLAST and a Java application manager for BLAST results. Bioinformatics. 2009. https://doi.org/10.1093/bioinformatics/btp067.

    Article  PubMed  Google Scholar 

  16. 16.

    Figshare. https://doi.org/10.6084/m9.figshare.10001870.v3.

  17. 17.

    SRA. https://www.ncbi.nlm.nih.gov/sra/?term=SRP150632.

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Acknowledgements

The authors thank Vasso Terzoglou, Katerina Oikonomaki and Eliza Kaitetzidou for recommendations during RNA extraction and Alexandros Tsakogiannis for useful discussions on transcriptome analysis. Sequencing service was provided by the Norwegian Sequencing Centre (http://www.sequencing.uio.no), a national technology platform hosted by the University of Oslo and supported by the “Functional Genomics” and “Infrastructure” programs of the Research Council of Norway and the Southeastern Regional Health Authorities.

Funding

This research is co-financed by Greece and the European Union (European Social Fund-ESF) through the Operational Programme «Human Resources Development, Education and Lifelong Learning» in the context of the project “Reinforcement of Postdoctoral Researchers” (MIS-5001552 - 7834), implemented by the State Scholarships Foundation (ΙΚΥ). Funding was awarded to the corresponding author in the form of a postdoctoral scholarship.

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TD conceived and managed the study; TD, VK, and TM designed experiments; TD provided live samples; VK and TD performed the experimental treatments; VK carried out laboratory analyses; SK performed library preparation and sequencing; VK, JL and TM analysed the data; CD, CT, CA, AM, provided access to research infrastructures; TM wrote the first draft of the paper and VK and TD made major contributions to the writing. All authors reviewed and contributed to the final version of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Thanos Dailianis.

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Manousaki, T., Koutsouveli, V., Lagnel, J. et al. A de novo transcriptome assembly for the bath sponge Spongia officinalis, adjusting for microsymbionts. BMC Res Notes 12, 813 (2019). https://doi.org/10.1186/s13104-019-4843-6

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Keywords

  • Porifera
  • Marine invertebrate
  • Prokaryotic symbionts
  • RNAseq
  • Heat stress
  • Bioinformatics