- Research article
- Open Access
Effects of epiplakin-knockdown in cultured corneal epithelial cells
© The Author(s). 2016
- Received: 27 July 2015
- Accepted: 10 May 2016
- Published: 20 May 2016
To investigate effects of knockdown of epiplakin gene expression on the homeostasis of cultured corneal epithelial cell line. We previously reported acceleration of corneal epithelial wound healing in an epiplakin-null mouse.
Gene expression of epiplakin was knockdowned by employing siRNA transfection in SV40-immortalized human corneal epithelial cell line. Protein expression of E-cadherin, keratin 6 and vimentin was examined by western blotting. Cell migration and proliferation were examined by using scratch assay and Alamar blue assay, respectively.
Scratch assay and Alamar blue assay showed migration and proliferation of the cells was accelerated by epiplakin knockdown. siRNA-knockdown of epiplakin suppressed protein expression of E-cadherin, keratin 6 and vimentin.
Decreased expression of E-cadherin, keratin 6 and vimentin might be included in the mechanisms of cell migration acceleration in the absence of epiplakin. The mechanism of cell proliferation stimulation by epiplakin knockdown is to be investigated.
- Corneal epithelium
Epiplakin was one of intermediate filament-related components and was originally identified as an autoantigen that reacted with serum from a patient with subepidermal blistering disease , . Epiplakin is homologous to plectin and other members of the plakin family , . Human epiplakin is a 552-kDa protein that is expressed in various epithelial tissues, i.e., epidermis, esophagus, outer root sheath of hair follicles and mucous epithelial cells , . Studies by using an epiplakin-null mouse line showed that lacking epiplakin accelerates migration of epidermal keratinocytes in mice in vivo and also showed that this is also the case in outgrowth of keratinocytes from explanted skin tissue in vitro, although the exact mechanism of the phenomena is to be revealed . We reported that corneal epithelium, a non-keratinizing stratified squamous epithelium, also express epiplakin and its loss accelerates cell migration-dependent healing of the corneal epithelium in mice. The in vivo study showed reduced E-cadherin protein and mRNA in epiplakin -null corneal epithelium, although E-cadherin expression level is not altered in epidermis by the loss of epiplakin . In the present study we investigated behaviors, i.e., cell migration and proliferation, of epiplakin-knockdowned cultured corneal epithelial cell line and also examined the expression level of E-cadherin, keratin 6 and vimentin, both members of intermediate filament involved in cell migration , in the cells, although decreased epiplakin expression does not solely explain the mechanism of cell migration acceleration in corneal epithelium.
Experimental protocols and the use of experimental mice were approved by the DNA Recombination Experiment Committee and the Animal Care and Use Committee of Wakayama Medical University and conducted in accordance with the Association for Research in Vision and Ophthalmology Statement for the Use of Animals in Ophthalmic and Vision Research.
Araki-Sasaki SV40-immortalized human corneal epithelial cell line was obtained from RIKEN Laboratories, Tsukuba Japan. The phenotype of this cell line retains many of the properties identified in their primary cell counterpart . They were grown to confluence in DMEM/F12 medium containing 200 U/ml of penicillin and streptomycin, 5 % FBS, 0.1 μg/ml cholera toxin, 5 μg/ml insulin, and 10 ng/ml human epidermal growth factor (EGF).
Expression of epiplakin was knockdowned in Araki-Sasaki cell line by employing a commercially available siRNA for epiplakin. The cells (2 × 105) in each well of a six well plate in 2 ml antibiotic-free normal growth medium supplemented with 5 % fetal bovine serum. The cells were incubate at 37 °C under 5.0 % CO2 until the cells are 60–80 % confluent. Human epiplakin 1 siRNA (sc-77799, Santa Cruz Biotechnology, CA) or control siRNA (sc-37007, Santa Cruz Biotechnology) was transfected for 6.0 h according to the protocol prepared by the manufacturer using siRNA Transfection Medium (sc-36868, Santa Cruz Biotechnology). Cells without siRNA were also prepared (control). Normal growth medium (1.0 ml) containing 2 times the normal serum and antibiotics concentration (2× normal growth medium) was added to each well without removing the transfection mixture.
The cells were cultured for 72 h after siRNA transfection procedure. The medium was then replaced with fresh 1× normal growth medium and processed for scratch assay as previously reported. Two liner defects were produced by a silicone needle in monolayer of the cells in control culture and siRNA epiplakin knockdown culture. Six cultures were prepared for each condition. The closure of the defect was evaluated at two independent points in each defect under phase-contrast microscopy at every 3 h until 30 h post-wounding. The width of the remaining defect was measured at four independent points at each time point and statistically analysed by Mann–Whitney U-test.
Araki-Sasaki cells were treated with epiplakin-siRNA or control siRNA as described above. Cells were seeded into wells of 96-well plates at the concentration of 1.0 ×104 cells/100 l/well 72 h after siRNA treatment, and incubated for 24 h. Twenty-six wells were prepared for each condition. Cell proliferation was assayed by using Alamar blue (Trek Diagnostic Systems, West Sussex, UK) according to the manufacturer’s protocol . After a wash with phosphate-buffered saline (PBS), 40 μL Alamar blue was diluted in culture medium (1:2).Thirty and sixty min later, the optical absorbance at 570 nm was measured at 570 nm.
The efficacy of knockdown of epiplakin protein expression was first evaluated by using western blotting with anti-epiplakin antibody (T-16, sc-87104, Santa Cruz:diluted 1:500) as previously reported . Then protein expression of E-cadherin, keratin 6 and vimentin was assayed by western blotting as previously reported . SDS-PAGE was done with the gel of Mini-PROTEAN® TGX™ Gel (Bio-Rad, #456-1096) under 200 V. The PVDF membrane was Immobilon-P Membrane, PVDF, 0.45 µm, 26 × 26 cm sheet.
(Merk Millipore Corporation, #IPVH304F0) and transfer the proteins under 5 V. Antibodies used were anti-E-cadherin antibody (G-10, sc-8426, Santa Cruz Biotechnology, diluted 1:1000) and anti-kerarin 6 antibody (LHK6, sc-53260, Santa Cruz Biotechnology, diluted 1:1000), and anti-vimentin antibody (C-20, sc-7557, Santa Cruz Biotechnology, diluted 1:1000).
Further study is to be conducted to uncover the mechanism of cell migration acceleration in the absence of epiplakin in vivo and in vitro in order to understand the biological mechanism underlying corneal epithelial wound healing.
SiRNA knockdown of epiplakin gene expression accelerated migration and proliferation of corneal epithelial cell in cell culture. Decreased expression of E-cadherin, keratin 6 and vimentin might be included in the mechanisms of cell migration acceleration in the absence of epiplakin. The mechanism of cell proliferation stimulation by epiplakin knockdown is to be investigated.
SS conceived and designed the experiments. MK and YO performed the experiments. TM and OY participated in study design, data analysis. All authors read and approved the final manuscript.
The authors thank Prof. Sakuhei Fujiwara, Department of Dermatology, Ohita University School of Medicine, Ohita, Japan, for his helpful suggestion.
The authors declare that they have no competing interests.
This study was supported by Grant of Wakayama Medical Award for Young Researchers (to MK) and the Grants from the Ministry of Education, Science, Sports and Culture of Japan (C21592241 to YO, C19592036 to SS). The abstract of the manuscript was presented by Dr. Kokado at the ARVO Annual meeting (2009, 2012, Fort Lauderdale, FL).
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Fujiwara S, Takeo N, Otani Y, et al. Epiplakin, a novel member of the Plakin family originally identified as a 450-kDa human epidermal autoantigen. Structure and tissue localization. J Biol Chem. 2001;276:13340–7.View ArticlePubMedGoogle Scholar
- Takeo N, Wang W, Matsuo N, Sumiyoshi H, Yoshioka H, Fujiwara S. Structure and heterogeneity of the human gene for epiplakin (EPPK1). J Invest Dermatol. 2003;121:1224–6.View ArticlePubMedGoogle Scholar
- Goto M, Sumiyoshi H, Sakai T, et al. Elimination of epiplakin by gene targeting results in acceleration of keratinocyte migration in mice. Mol Cell Biol. 2006;26:548–58.View ArticlePubMedPubMed CentralGoogle Scholar
- Kokado M, Okada Y, Goto M, Ishikawa K, Miyamoto T, et al. Increased fragility, impaired differentiation, and acceleration of migration of corneal epithelium of epiplakin-null mice. Invest Ophthalmol Vis Sci. 2013;54:3780–9.View ArticlePubMedGoogle Scholar
- Velez-delValle C, Marsch-Moreno M, Castro-Muñozledo F, et al. Epithelial cell migration requires the interaction between the vimentin and keratin intermediate filaments. Sci Rep. 2016;13(6):24389.View ArticleGoogle Scholar
- Araki-Sasaki K, Ohashi Y, Sasabe T, Hayashi K, Watanabe H, Tano Y, Handa H. An SV40-immortalized human corneal epithelial cell line and its characterization. Invest Ophthalmol Vis Sci. 1995;36:614–21.PubMedGoogle Scholar
- Hamid R, Rotshteyn Y, Rabadi L, et al. Comparison of Alamar blue and MTT assays for high through-put screening. Toxicol In Vitro. 2004;18:703–10.View ArticlePubMedGoogle Scholar
- Okada Y, Shirai K, Reinach PS, Kitano-Izutani A, Miyajima M, Flanders KC, Jester JV, Tominaga M, Saika S. TRPA1 is required for TGF-β signaling and its loss blocks inflammatory fibrosis in mouse corneal stroma. Lab Invest. 2014;94:1030–41.View ArticlePubMedGoogle Scholar
- Sharma GD, He J, Bazan HE. p38 and ERK1/2 coordinate cellular migration and proliferation in epithelial wound healing: evidence of cross-talk activation between MAP kinase cascades. J Biol Chem. 2003;278:21989–97.View ArticlePubMedGoogle Scholar
- Saika S, Okada Y, Miyamoto T, Yamanaka O, Ohnishi Y, Ooshima A, Liu CY, Weng D, Kao WW. Role of p38 MAP kinase in regulation of cell migration and proliferation in healing corneal epithelium. Invest Ophthalmol Vis Sci. 2004;45:100–9.View ArticlePubMedGoogle Scholar
- Okada Y, Saika S, Shirai K, Yamanaka O, Kitano A, Wang Z, Yang H, Reinach P. JNK MAPK signaling contributes in vivo to injury-induced corneal epithelial migration. Ophthalmic Res. 2009;42:185–92.View ArticlePubMedGoogle Scholar
- Lyu J, Joo CK. Wnt-7a up-regulates matrix metalloproteinase-12 expression and promotes cell proliferation in corneal epithelial cells during wound healing. J Biol Chem. 2005;280:21653–60.View ArticlePubMedGoogle Scholar
- Tsang SM, Liu L, Teh MT, et al: Desmoglein3, via an interaction with E-cadherin, is associated with activation of Src. PLoS One. 2010. doi:10.1371/journal.pone.0014211.Google Scholar
- Mao Z, Ma X, Rong Y, et al. Connective tissue growth factor enhances the migration of gastric cancer through downregulation of E-cadherin via the NF-κB pathway. Cancer Sci. 2011;102:104–10.View ArticlePubMedGoogle Scholar
- Kim NH, Kim SN, Kim YK. Involvement of HDAC1 in E-cadherin expression in prostate cancer cells; its implication for cell motility and invasion. Biochem Biophys Res Commun. 2011;404:915–21.View ArticlePubMedGoogle Scholar
- Wan H, South AP, Hart IR. Increased keratinocyte proliferation initiated through downregulation of desmoplakin by RNA interference. Exp Cell Res. 2007;313:2336–44.View ArticlePubMedGoogle Scholar
- Chidgey M, Brakebusch C, Gustafsson E, Cruchley A, Hail C, Kirk S, Merritt A, North A, Tselepis C, Hewitt J, Byrne C, Fassler R, Garrod D. Mice lacking desmocollin 1 show epidermal fragility accompanied by barrier defects and abnormal differentiation. J Cell Biol. 2001;155:821–32.View ArticlePubMedPubMed CentralGoogle Scholar
- Merritt AJ, Berika MY, Zhai W, et al. Suprabasal desmoglein 3 expression in the epidermis of transgenic mice results in hyperproliferation and abnormal differentiation. Mol Cell Biol. 2002;22:5846–58.View ArticlePubMedPubMed CentralGoogle Scholar
- Brennan D, Hu Y, Joubeh S, et al. Suprabasal Dsg2 expression in transgenic mouse skin confers a hyperproliferative and apoptosis-resistant phenotype to keratinocytes. J Cell Sci. 2007;120:758–71.View ArticlePubMedGoogle Scholar
- Kolly C, Zakher A, Strauss C, Suter MM, Müller EJ. Keratinocyte transcriptional regulation of the human c-Myc promoter occurs via a novel Lef/Tcf binding element distinct from neoplastic cells. FEBS Lett. 2007;581:1969–76.View ArticlePubMedGoogle Scholar
- Dmello C, Sawant S, Alam H, Gangadaran P, Tiwari R, Dongre H, Rana N, Barve S, Costea DE, Chaukar D, Kane S, Pant H, Vaidya M. Vimentin-mediated regulation of cell motility through modulation of beta4 integrin protein levels in oral tumor derived cells. Int J Biochem Cell Biol. 2016;70:161–72.View ArticlePubMedGoogle Scholar