Skip to main content
Download PDF

Phage typing or CRISPR typing for epidemiological surveillance of Salmonella Typhimurium?

BMC Research Notes volume 10, Article number: 578 (2017) Cite this article

Abstract

Objective

Salmonella Typhimurium is the most dominant Salmonella serovar around the world. It is associated with foodborne gastroenteritis outbreaks but has recently been associated with invasive illness and deaths. Characterization of S. Typhimurium is therefore very crucial for epidemiological surveillance. Phage typing has been used for decades for subtyping of S. Typhimurium to determine the epidemiological relation among isolates. Recent studies however have suggested that high throughput clustered regular interspaced short palindromic repeats (CRISPR) typing has the potential to replace phage typing. This study aimed to determine the efficacy of high-throughput CRISPR typing over conventional phage typing in epidemiological surveillance and outbreak investigation of S. Typhimurium.

Results

In silico analysis of whole genome sequences (WGS) of well-documented phage types of S. Typhimurium reveals the presence of different CRISPR type among strains belong to the same phage type. Furthermore, different phage types of S. Typhimurium share identical CRISPR type. Interestingly, identical spacers were detected among outbreak and non-outbreak associated DT8 strains of S. Typhimurium. Therefore, CRISPR typing is not useful for the epidemiological surveillance and outbreak investigation of S. Typhimurium and phage typing, until it is replaced by WGS, is still the gold standard method for epidemiological surveillance of S. Typhimurium.

Introduction

Salmonellosis is one of the most common causes of foodborne disease worldwide. Nontyphoidal salmonellosis (NTS) is a zoonotic disease transmitted from animals to humans through consumption of contaminated food. Worldwide, Salmonella enterica serovar Typhimurium (S. Typhimurium) accounts for most human infection of NTS and has been associated with foodborne outbreaks in developing and developed countries resulting in high morbidity and mortality [1]. Furthermore, the recent emergence of the multidrug-resistant (MDR) S. Typhimurium variant of a distinct Sequence Type ST313 in sub-Saharan Africa represents a major public health concern as it is associated with invasive illness and deaths [2]. An efficient laboratory system for epidemiological surveillance and outbreak investigation of Salmonella Typhimurium is therefore very crucial.

Phage typing system is a phenotypical method that has been used for decades for subtyping of S. Typhimurium to determine the epidemiological relation among isolates [3]. Phage typing is a rapid and low cost approach for the epidemiological surveillance and outbreak investigation of S. Typhimurium. The system distinguishes more than 300 definitive phage types (DT) of S. Typhimurium based on their patterns of lysis to a unique collection of Salmonella phages but it has shown some limitations including the maintenance of typing phages by the reference laboratory and the updating of the system furthermore it depends entirely on the experience of the individual laboratory for interpretation of the results [4].

Recent studies have suggested that high throughput clustered regular interspaced short palindromic repeats (CRISPRs) typing and the microbead-based CRISPOL assay have the potential to replace traditional bacterial typing and subtyping systems including phage typing [5, 6]. CRISPRs consist of direct repeats (DRs) separated by variable spacer sequences that are derived from foreign phages or plasmids [7] while CRISPOL is a bead-based liquid hybridization assay for CRISPR polymorphism [5].

A recent study reported identical CRISPRs between two different phage types of S. Typhimurium; DT8 and DT30 [8] which reveals the limitations of CRISPR typing for epidemiological surveillance of S. Typhimurium.

This study aimed to analyze the CRISPR/CRISPOL type of well-documented phage types of S. Typhimurium in order to determine the efficacy of high-throughput CRISPR and CRISPOL typing over conventional phage typing in epidemiological surveillance of S. Typhimurium.

Main text

Methods

Whole genome sequence of different phage types of S. Typhimurium

The whole genome sequence of well-documented phage types of S. Typhimurium (Tables 1, 2) were obtained from Enterobase (https://enterobase.warwick.ac.uk/). Furthermore, a set of different phage types of S. Typhimurium that are used as control in Anderson phage typing scheme (Tables 1, 2) were selected for whole genome sequencing (WGS). Genomic DNA was extracted using QIAamp DNA Mini Kit (Qiagen) according to manufacturer’s instructions and submitted for WGS using an Illumina MiSeq on 250 bp paired-end (PE) libraries. The quality of PE data was evaluated using FastQC toolkit (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/). Adapter sequences were removed using ea-utils package (https://expressionanalysis.github.io/ea-utils/). PE reads for each isolate were de novo assembled using velvet [9]. The best assembly with the highest N50 value was obtained. Raw sequence data of control phage types of S. Typhimurium have been submitted to the European Nucleotide Archive (ENA) under study Accession No.: PRJEB18673 (http://www.ebi.ac.uk/ena/data/view/PRJEB18673) and also available via Enterobase (https://enterobase.warwick.ac.uk/).

Table 1 Salmonella Typhimurium strains belonging to the same phage type show different CRISPR/CRISPOL type
Full size table
Table 2 Salmonella Typhimurium strains belonging to different phage types show identical CRISPR/CRISPOL type
Full size table

In silico CRISPR and CRISPOL analysis

PE reads of different phage types of S. Typhimurium were also assembled using Enterobase (https://enterobase.warwick.ac.uk/) where CRISPRs and CRISPOL were called directly from the raw reads rather than the assembly.

Enterobase was used to determine the CRISPR type and CRISPOL type of all phage types of S. Typhimurium. In Enterobase, each phage type of S. Typhimurium was assigned unique accession number (Tables 1, 2).

Previously, sequenced CRISPR loci of different phage types of S. Typhimurium using polymerase chain reaction (PCR) [5] were also included in this study (Table 3).

Table 3 CRISPOL type among different phage types of S. Typhimurium
Full size table

PE reads of S. Typhimurium phage type DT8 associated with a foodborne outbreak in the summer of 2013 in the States of Jersey [10] were downloaded from ENA; study Accession Number PRJNA248792 (http://www.ebi.ac.uk/ena/data/view/PRJNA248792) and assembled by Enterobase. CRISPR and CRISPOL types were determined for all outbreak strains using Enterobase (Additional file 1: Table S1).

Spacers sequence within the assembled genomes of outbreak and non-outbreak associated DT8 strains were also characterized using CRISPRFinder (http://crispr.i2bc.paris-saclay.fr/Server/) (Additional file 1: Table S1).

Results

In silico analysis of genome sequences of control and well documented phage types of S. Typhimurium revealed two CRISPR loci, CRISPR-1 and CRISPR-2, within all phage types of S. Typhimurium. Although DRs are almost identical among all phage types of S. Typhimurium spacers sequences within the CRISPR loci are not unique to the phage type as strains belong to the same phage type have different spacers and subsequently different CRISPR/CRISPOL type (Table 1) furthermore, different phage types have identical spacers and same CRISPR/CRISPOL type (Table 2).

Different CRISPR/CRISPOL type within the same phage type of S. Typhimurium

In Table 1, three strains of S. Typhimurium that belong to phage type DT1 including strains DT1, TM 68-619 and TM 65-111 have different spacers and subsequently show different CRISPR/CRISPOL type; 8579/430, 2536/54 and 7387/90 respectively. Two strains belong to phage type DT10 have different CRISPR/CRISPOL type; MS34 (9509/1629) and S81-784 (9913/1688). Two strains belong to phage type DT15a have different CRISPR/CRISPOL type; 9517/1634 in isolate MS41 and 9916/1756 in isolate S81-798. Moreover, three strains belong to DT41 have different CRISPR/CRISPOL type; 9513/1630 in isolate M11-2004, 7434/223 in isolate CQ 41 and 9929/1766 in isolate S02-0321.

Identical CRISPR/CRISPOL type within different phage types of S. Typhimurium

CRISPR/CRISPOL type among phage types DT8 and DT30

Identical spacers were detected among different phage types of S. Typhimurium. For example, three strains of DT8 including M12-2001, M15-2006 and MS32 have the same CRISPR/CRISPOL type (812/250) as a strain belongs to phage type DT30 (MS57). Moreover, different strains belong to phage type DT8 have different CRISPR/CRISPOL type; M18-2003 (1069/6) and MS150057 (2260/708) (Table 2).

Interestingly, S. Typhimurium DT8 strains associated with the foodborne outbreak in the summer of 2013 in the States of Jersey [10] showed identical CRISPR/CRISPOL type (1069/6) however, the same CRISPR/CRISPOL type were reported in other DT8 strains that do not belong to the outbreak as confirmed by WGS [10]. Identical spacers were detected among outbreak associated and non-outbreak associated DT8 strains (Additional file 1: Table S1).

CRISPR/CRISPOL type among phage types DT104, DT104b and U302

Variations in the CRISPR/CRISPOL type among strains of the same phage type such as DT104 and DT104b have been also noticed (Table 2). Although three strains of S. Typhimurium phage type DT104 including TM75-339, MS150098 and MS150095, have identical spacer sequences and CRISPR/CRISPOL type (12/21) the same CRISPR/CRISPOL type is present in different phage types including U302 (M18-2006; 12/21) and DT104b (MS130531; 12/21).

CRISPR/CRISPOL type among phage types DT40, DT56, DT99 and U319

Strains of S. Typhimurium belong to different phage types such as DT99, DT56, U319 and DT40 (S05-2864) have identical spacer sequences and identical CRISPR/CRISPOL type (7433/14). Moreover, several strains belong to phage type DT40 including S05-2864, M20-2006, M19-2003 and CQ 40 have different CRISPR/CRISPOL type; 7433/14, 9520/1637, 9519/1636 and 745/18 respectively (Table 2).

CRISPR/CRISPOL type among phage types DT7a, DT20a, DT120, DT193 and untypable strains

In Table 2, strains of S. Typhimurium belong to phage type DT120 have different spacers and subsequently different CRISPR/CRISPOL type including S02-3776 (9921/1759), 07_2198 (9911/1753), M16-2000 (9510/1428), and S/20160374 (322/1).

Interestingly, a strains of phage type DT120 (M16-2000) has identical spacers and CRISPR/CRISPOL type (9510/1428) as another strain belongs to phage type DT7a (MS120840). Moreover, some strains belong to phage types DT120 (S/20160374 and S/20160407), DT20a (MS150110), DT193 (MS150007) and untypable strain (MS150097) have identical spacers and therefore share the same CRISPR/CRISPOL type (322/1). Different strains belong to phage type DT193 have different spacers and CRISPR/CRISPOL type; MS150007 (322/1) and MS150252 (317/2).

CRISPR/CRISPOL type among phage types DT3, DT12 and DT193a

Some strains of phage types DT12 (DT12) and DT3 (S81-482) have identical spacers and identical CRISPR/CRISPOL type; 5268/19. Moreover, a strain belongs to DT12 (S02-2651) has identical CRISPR/CRISPOL type, 774/46, as a strain belongs to phage type DT193a (MS120454) (Table 2).

CRISPR/CRISPOL type among phage types DT135, DT191a and RDNC

Identical spacer sequences and CRISPR/CRISPOL type (91/4) were detected in different phage types of S. Typhimurium including DT135 (MS150112 and MS150180), DT191a (DT19a) and strains that react with phages but do not confirm to recognized pattern (RDNC) (MS150102 and MS150230). Furthermore, other strains belong to phage type DT135 show different spacers and subsequently different CRISPR/CRISPOL type; 5753/396 in DT135 and 3247/66 in MS150100 (Table 2).

CRISPOL assay confirms the no relation among phage type and CRISPRs

CRISPOL assay developed by Fabre et al. [5] when carried out on representative phage types of S. Typhimurium it reveals that there is no relation among the phage type and the CRISPOL type as strains belong to the same phage type have different CRISPOL type as seen in DT104 strains (Table 3). On the other hand, different phage types including DT7, DT193, U311, DT41 showed identical CRISPOL type as ‘1’ (Table 3).

Discussion

Salmonella Typhimurium is the most dominant Salmonella serovar around the world and has been associated with foodborne outbreaks in both developing and high-income countries [1, 11] and infection can result in bacteraemia and invasive disease [12, 13]. Epidemiological characterization of S. Typhimurium is therefore very crucial for the surveillance and outbreak investigation.

Phage typing system [3] has been a very useful phenotypical, definitive method for epidemiological characterization of S. Typhimurium and identification of the source of infection [14,15,16,17]. Although it has been suggested that the high throughput CRISPR typing and subtyping have the potential to replace traditional phage typing [5] this study demonstrates that It is impossible for CRISPR typing and CRISPOl assay to replace phage typing for epidemiological characterization of S. Typhimurium as there is no correlation between the phage type and the CRISPR/CRISPOL type.

Interestingly, S. Typhimurium DT8 strains associated with the foodborne outbreak in the summer of 2013 in the States of Jersey [10] showed identical CRISPR/CRISPOL type however, the same CRISPR/CRISPOL type were reported in other DT8 strains that do not belong to the outbreak as confirmed by WGS [10]. Detection of identical spacers among outbreak associated and non-outbreak associated DT8 strains reveals the limitation of CRISPR typing and subtyping in investigation of outbreaks.

The MDR DT104 strain of S. Typhimurium has been associated with foodborne outbreaks all over the world and phage typing was very successful in epidemiological characterization of the outbreak and identification of the source [18,19,20] however in this study strains belong to DT104 showed different spacers and subsequently different CRISPR/CRISPOL type therefore CRISPR typing and CRISPOL assay cannot be used in public health laboratories to determine the epidemiological relation among S. Typhimurium isolates.

The presence of CRISPR/CRISPOL type within the same phage type and the presence of identical spacers among different phage types of S. Typhimurium confirms the limitations of CRISPR typing and subtyping for the epidemiological surveillance and outbreak investigation of S. Typhimurium.

There is no doubt that rapid WGS will shape the future of diagnostic microbiology as it has the potential to replace the routine typing and subtyping methods including Anderson phage typing system for the surveillance of outbreaks caused by different Salmonella serovars in real-time [10, 21, 22]. However, in the meantime, traditional phage typing scheme of S. Typhimurium remains the gold standard method for subtyping of S. Typhimurium for laboratory surveillance and outbreak investigation despite its technical limitations. Furthermore, it represents an ideal model for studying the complex dynamics of phage-host interaction [8].

In conclusion, high throughput CRISPR/CRISPOL typing might be useful for the discrimination among different Salmonella serovars however it is not useful for the epidemiological surveillance and outbreak investigation of S. Typhimurium and phage typing, until it is replaced by WGS, is still the gold standard method for epidemiological surveillance of S. Typhimurium.

Limitations

More outbreaks of S. Typhimurium caused by phage types other than DT8 can be included to confirm the unsuitability of CRISPR typing in epidemiological surveillance and outbreak investigation of S. Typhimurium.

Abbreviations

CRISPR:

clustered regular interspaced short palindromic repeats

DT:

phage type

MDR:

multidrug resistant

NSSLRL:

National Salmonella Shigella Listeria Reference Laboratory

NTS:

nontyphoidal salmonella

PE:

paired end

PCR:

polymerase chain reaction

RDNC:

strains that react with phages but do not confirm to recognized pattern

SSSCDRL:

Scottish Salmonella, Shigella and Clostridium difficile Reference Laboratory

S. Typhimurium:

Salmonella Typhimurium

WGS:

whole genome sequencing

References

  1. Majowicz SE, Musto J, Scallan E, Angulo FJ, Kirk M, O’Brien SJ, Jones TF, Fazil A, Hoekstra RM. International collaboration on enteric disease “burden of illness” S: the global burden of nontyphoidal Salmonella gastroenteritis. Clin Infect Dis. 2010;50(6):882–9.

    Article  PubMed  Google Scholar 

  2. Okoro CK, Kingsley RA, Connor TR, Harris SR, Parry CM, Al-Mashhadani MN, Kariuki S, Msefula CL, Gordon MA, de Pinna E, et al. Intracontinental spread of human invasive Salmonella typhimurium pathovariants in sub-Saharan Africa. Nat Genet. 2012;44(11):1215–21.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  3. Anderson ES, Ward LR, Saxe MJ, de Sa JD. Bacteriophage-typing designations of Salmonella typhimurium. J Hyg (Lond). 1977;78(2):297–300.

    CAS  Article  Google Scholar 

  4. Baggesen DL, Sorensen G, Nielsen EM, Wegener HC. Phage typing of Salmonella typhimurium—is it still a useful tool for surveillance and outbreak investigation? Euro surveillance: bulletin European sur les maladies transmissibles. Eur Commun Dis Bull. 2010;15(4):19471.

    CAS  Google Scholar 

  5. Fabre L, Zhang J, Guigon G, Le Hello S, Guibert V, Accou-Demartin M, de Romans S, Lim C, Roux C, Passet V, et al. CRISPR typing and subtyping for improved laboratory surveillance of Salmonella infections. PLoS ONE. 2012;7(5):e36995.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  6. Shariat N, Dudley EG. CRISPRs: molecular signatures used for pathogen subtyping. Appl Environ Microbiol. 2014;80(2):430–9.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  7. Jansen R, Embden JD, Gaastra W, Schouls LM. Identification of genes that are associated with DNA repeats in prokaryotes. Mol Microbiol. 2002;43(6):1565–75.

    CAS  Article  PubMed  Google Scholar 

  8. Mohammed M, Cormican M. Whole genome sequencing provides possible explanations for the difference in phage susceptibility among two Salmonella typhimurium phage types (DT8 and DT30) associated with a single foodborne outbreak. BMC Res Notes. 2015;8:728.

    Article  PubMed  PubMed Central  Google Scholar 

  9. Zerbino DR, Birney E. Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res. 2008;18(5):821–9.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  10. Ashton PM, Nair S, Peters TM, Bale JA, Powell DG, Painset A, Tewolde R, Schaefer U, Jenkins C, Dallman TJ, et al. Identification of Salmonella for public health surveillance using whole genome sequencing. PeerJ. 2016;4:e1752.

    Article  PubMed  PubMed Central  Google Scholar 

  11. Galanis E, Lo Fo Wong DM, Patrick ME, Binsztein N, Cieslik A, Chalermchikit T, Aidara-Kane A, Ellis A, Angulo FJ, Wegener HC, et al. Web-based surveillance and global Salmonella distribution, 2000–2002. Emerg Infect Dis. 2006;12(3):381–8.

    Article  PubMed  PubMed Central  Google Scholar 

  12. Ao TT, Feasey NA, Gordon MA, Keddy KH, Angulo FJ, Crump JA. Global burden of invasive nontyphoidal Salmonella disease, 2010. Emerg Infect Dis. 2015;21(6):941.

    CAS  Article  PubMed Central  Google Scholar 

  13. Tapia MD, Tennant SM, Bornstein K, Onwuchekwa U, Tamboura B, Maiga A, Sylla MB, Sissoko S, Kourouma N, Toure A, et al. Invasive nontyphoidal Salmonella infections among children in Mali, 2002–2014: microbiological and epidemiologic features guide vaccine development. Clin Infect Dis. 2015;61(Suppl 4):S332–8.

    Article  PubMed  PubMed Central  Google Scholar 

  14. Fielding JE, Snell P, Milazzo A, Del Fabbro L, Raupach J. An outbreak of Salmonella typhimurium phage type 4 linked to cold set cheesecake. Commun Dis Intell Q Rep. 2003;27(4):513–4.

    PubMed  Google Scholar 

  15. Tribe IG, Tsimogiannis H, Mmolawa P, Davos D. An outbreak of Salmonella typhimurium phage type 29 linked to a noodle restaurant in South Australia. Commun Dis Intell Q Rep. 2001;25(2):72.

    CAS  PubMed  Google Scholar 

  16. Moffatt CR, Combs BG, Mwanri L, Holland R, Delroy B, Cameron S, Givney RC. An outbreak of Salmonella typhimurium phage type 64 gastroenteritis linked to catered luncheons in Adelaide, South Australia, June 2005. Commun Dis Intell Q Rep. 2006;30(4):443–8.

    PubMed  Google Scholar 

  17. Boxall NS, Adak GK. E DEP, Gillespie IA: a Salmonella typhimurium phage type (PT) U320 outbreak in England, 2008: continuation of a trend involving ready-to-eat products. Epidemiol Infect. 2011;139(12):1936–44.

    CAS  Article  PubMed  Google Scholar 

  18. Molbak K, Baggesen DL, Aarestrup FM, Ebbesen JM, Engberg J, Frydendahl K, Gerner-Smidt P, Petersen AM, Wegener HC. An outbreak of multidrug-resistant, quinolone-resistant Salmonella enterica serotype typhimurium DT104. N Engl J Med. 1999;341(19):1420–5.

    CAS  Article  PubMed  Google Scholar 

  19. Cody SH, Abbott SL, Marfin AA, Schulz B, Wagner P, Robbins K, Mohle-Boetani JC, Vugia DJ. Two outbreaks of multidrug-resistant Salmonella serotype typhimurium DT104 infections linked to raw-milk cheese in northern California. JAMA. 1999;281(19):1805–10.

    CAS  Article  PubMed  Google Scholar 

  20. Dechet AM, Scallan E, Gensheimer K, Hoekstra R, Gunderman-King J, Lockett J, Wrigley D, Chege W, Sobel J, Multistate Working G. Outbreak of multidrug-resistant Salmonella enterica serotype typhimurium definitive type 104 infection linked to commercial ground beef, northeastern United States, 2003–2004. Clin Infect Dis. 2006;42(6):747–52.

    Article  PubMed  Google Scholar 

  21. Mohammed M, Delappe N, O’Connor J, McKeown P, Garvey P, Cormican M. Whole genome sequencing provides an unambiguous link between Salmonella Dublin outbreak strain and a historical isolate. Epidemiol Infect 2016;144(3):576–81.

    CAS  Article  PubMed  Google Scholar 

  22. Wuyts V, Denayer S, Roosens NH, Mattheus W, Bertrand S, Marchal K, Dierick K, De Keersmaecker SC. Whole genome sequence analysis of Salmonella enteritidis PT4 outbreaks from a National Reference Laboratory’s Viewpoint. PLoS curr 2015. https://doi.org/10.1371/currents.outbreaks.aa5372d90826e6cb0136ff66bb7a62fc.

    Google Scholar 

Download references

Acknowledgements

Not applicable.

Competing interests

The author no competing interests.

Availability of data and materials

Raw sequence data of control phage types of S. Typhimurium will be publically available via ENA under study Accession No.: PRJEB18673 (http://www.ebi.ac.uk/ena/data/view/PRJEB18673) and also available via Enterobase (https://enterobase.warwick.ac.uk/). All sequencing data is available on request.

Consent to publish

Not applicable.

Ethical approval and consent

Not applicable.

Funding

Not applicable.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Author information

Authors and Affiliations

  1. Department of Biomedical Sciences, Faculty of Science and Technology, University of Westminster, London, UK

    Manal Mohammed

Authors
  1. Manal Mohammed
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Manal Mohammed.

Additional file

13104_2017_2878_MOESM1_ESM.docx

Additional file 1: Table S1. CRISPR and CRISPOL types of outbreak and non-outbreak associated DT8 strains of S. Typhimurium. Identical CRISPR and CRISPOL types were detected among outbreak and non-outbreak strains.

Rights and permissions

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Mohammed, M. Phage typing or CRISPR typing for epidemiological surveillance of Salmonella Typhimurium?. BMC Res Notes 10, 578 (2017). https://doi.org/10.1186/s13104-017-2878-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/s13104-017-2878-0

Keywords

  • Salmonella Typhimurium
  • CRISPR typing
  • Phage typing
  • Surveillance
  • Outbreaks

BMC Research Notes

ISSN: 1756-0500

Contact us