Diarrhoeal disease. http://www.who.int/mediacentre/factsheets/fs330/en/. Accessed 4 April 2018.
Juhas M. Horizontal gene transfer in human pathogens. Crit Rev Microbiol. 2015;41(1):101–8.
Article
PubMed
CAS
Google Scholar
Tauxe RV. Emerging foodborne pathogens. Int J Food Microbiol. 2002;78(1):31–41.
Article
PubMed
CAS
Google Scholar
Chen J, Carpena N, Quiles-Puchalt N, Ram G, Novick RP, Penadés JR. Intra- and inter-generic transfer of pathogenicity island-encoded virulence genes by cos phages. ISME J. 2014;9:1260.
Article
PubMed
PubMed Central
CAS
Google Scholar
Lai YS, Rosenshine I, Leong JM, Frankel G. Intimate host attachment: enteropathogenic and enterohaemorrhagic Escherichia coli. Cell Microbiol. 2013;15(11):1796–808.
PubMed
PubMed Central
CAS
Google Scholar
Schmidt MA. LEEways: tales of EPEC, ATEC and EHEC. Cell Microbiol. 2010;12(11):1544–52.
Article
PubMed
CAS
Google Scholar
Castro VS, Carvalho RCT, Conte CA, Figuiredo EES. Shiga-toxin producing Escherichia coli: pathogenicity, supershedding, diagnostic methods, occurrence, and foodborne outbreaks. Compr Rev Food Sci Food Saf. 2017;16(6):1269–80.
Article
CAS
Google Scholar
Patzi-Vargas S, Zaidi MB, Perez-Martinez I, León-Cen M, Michel-Ayala A, Chaussabel D, Estrada-Garcia T. Diarrheagenic Escherichia coli carrying supplementary virulence genes are an important cause of moderate to severe diarrhoeal disease in Mexico. PLoS Negl Trop Dis. 2015;9(3):e0003510.
Article
PubMed
PubMed Central
CAS
Google Scholar
Scheutz F, Teel LD, Beutin L, Piérard D, Buvens G, Karch H, Mellmann A, Caprioli A, Tozzoli R, Morabito S, et al. Multicenter evaluation of a sequence-based protocol for subtyping Shiga toxins and standardizing Stx nomenclature. J Clin Microbiol. 2012;50(9):2951–63.
Article
PubMed
PubMed Central
CAS
Google Scholar
Johnson RP, Clarke RC, Wilson JB, Read SC, Rahn K, Renwick SA, Sandhu KA, Alves D, Karmali MA, Lior H, et al. Growing concerns and recent outbreaks involving non-O157:H7 serotypes of verotoxigenic Escherichia coli. J Food Prot. 1996;59(10):1112–22.
Article
Google Scholar
Parsons BD, Zelyas N, Berenger BM, Chui L. Detection, characterization, and typing of Shiga toxin-producing Escherichia coli. Front Microbiol. 2016. https://doi.org/10.3389/fmicb.2016.00478.
Article
PubMed
PubMed Central
Google Scholar
Verstraete K, Van Coillie E, Werbrouck H, Van Weyenberg S, Herman L, Del-Favero J, De Rijk P, De Zutter L, Joris M-A, Heyndrickx M, et al. A qPCR assay to detect and quantify Shiga toxin-producing E. coli (STEC) in cattle and on farms: a potential predictive tool for STEC culture-positive farms. Toxins. 2014;6(4):1201–21.
Article
PubMed
PubMed Central
CAS
Google Scholar
Brusa V, Galli L, Linares LH, Ortega EE, Lirón JP, Leotta GA. Development and validation of two SYBR green PCR assays and a multiplex real-time PCR for the detection of Shiga toxin-producing Escherichia coli in meat. J Microbiol Methods. 2015;119:10–7.
Article
PubMed
CAS
Google Scholar
Singh P, Mustapha A. Multiplex real-time PCR assays for detection of eight Shiga toxin-producing Escherichia coli in food samples by melting curve analysis. Int J Food Microbiol. 2015;215:101–8.
Article
PubMed
CAS
Google Scholar
Salinas-Ibáñez ÁG, Lucero-Estrada C, Chialva C, Zárate JM, Juri-Ayub M, Escudero ME. Design of an internal amplification control for a duplex PCR used in the detection of Shiga toxin producing Escherichia coli in pediatric feces. Mol Cell Probes. 2015;29(6):351–7.
Article
PubMed
CAS
Google Scholar
Li B, Liu H, Wang W. Multiplex real-time PCR assay for detection of Escherichia coli O157:H7 and screening for non-O157 Shiga toxin-producing E. coli. BMC Microbiol. 2017;17(1):215.
Article
PubMed
PubMed Central
Google Scholar
Wang G, Clark CG, Rodgers FG. Detection in Escherichia coli of the genes encoding the major virulence factors, the genes defining the O157:H7 serotype, and components of the type 2 Shiga toxin family by multiplex PCR. J Clin Microbiol. 2002;40(10):3613–9.
Article
PubMed
PubMed Central
CAS
Google Scholar
Brandal LT, Lindstedt BA, Aas L, Stavnes TL, Lassen J, Kapperud G. Octaplex PCR and fluorescence-based capillary electrophoresis for identification of human diarrheagenic Escherichia coli and Shigella spp. J Microbiol Methods. 2007;68(2):331–41.
Article
PubMed
CAS
Google Scholar
Kong RYC, So CL, Law WF, Wu RSS. A sensitive and versatile multiplex PCR system for the rapid detection of enterotoxigenic (ETEC), enterohaemorrhagic (EHEC) and enteropathogenic (EPEC) strains of Escherichia coli. Mar Pollut Bull. 1999;38(12):1207–15.
Article
CAS
Google Scholar
Heijnen L, Medema G. Quantitative detection of E. coli, E. coli O157 and other Shiga toxin producing E. coli in water samples using a culture method combined with real-time PCR. J Water Health. 2006;4(4):487–98.
Article
PubMed
Google Scholar
Riyaz-Ul-Hassan S, Syed S, Johri S, Verma V, Qazi GN. Application of a multiplex PCR assay for the detection of Shigella, Escherichia coli and Shiga toxin-producing Esch. coli in milk. J Dairy Res. 2009;76(2):188–94.
Article
PubMed
CAS
Google Scholar
Ashbolt NJ, Grabow WOK, Snozzi M: Indicators of microbial water quality. In: Fewtrell L, Bartram J, editors. Water Quality Guidelines, Standards and Health: assessment of risk and risk management for water-related infectious disease. WHO edition. IWA and WHO; 2001. p. 289–316.
Corpet F. Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res. 1988;16(22):10881–90.
Article
PubMed
PubMed Central
CAS
Google Scholar
Markoulatos P, Siafakas N, Moncany M. Multiplex polymerase chain reaction: a practical approach. J Clin Lab Anal. 2002;16(1):47–51.
Article
PubMed
CAS
Google Scholar
Roux KH. Optimization and troubleshooting in PCR. PCR Methods Appl. 1995;4(5):S185–94.
Article
PubMed
CAS
Google Scholar
Edwards MC, Gibbs RA. Multiplex PCR. In: Dieffenbach CW, Dveksler GS, editors. PCR primer, a laboratory manual. New York: Cold Spring Harbor Laboratory Press; 1995. p. 157–71.
Google Scholar
Kwok S, Chang SY, Sninsky JJ, Wang A. A guide to the design and use of mismatched and degenerate primers. PCR Methods Appl. 1994;3(4):S39–47.
Article
PubMed
CAS
Google Scholar
Anglès d’Auriac M. Development of microbiological molecular diagnostic techniques for the rapid screening and identification of selected human bacterial pathogens and indicators. Oslo: University of Oslo; 2009.
Google Scholar
Sarkar G, Kapelner S, Sommer SS. Formamide can dramatically improve the specificity of PCR. Nucleic Acids Res. 1990;18(24):7465.
Article
PubMed
PubMed Central
CAS
Google Scholar
Abu Al-Soud W, Radstrom P. Effects of amplification facilitators on diagnostic PCR in the presence of blood, feces, and meat. J Clin Microbiol. 2000;38(12):4463–70.
PubMed
PubMed Central
CAS
Google Scholar
Kunin CM. Separation, characterization, and biological significance of a common antigen in Enterobacteriaceae. J Exp Med. 1963;85:541–8.
CAS
Google Scholar
Kuhn H-M, Meier-Dieter U, Mayer H. ECA, the enterobacterial common antigen. FEMS Microbiol Lett. 1988;54(3):195–222.
Article
CAS
Google Scholar
Ohta M, Ina K, Kusuzaki K, Kido N, Arakawa Y, Kato N. Cloning and expression of the rfe-rff gene cluster of Escherichia coli. Mol Microbiol. 1991;5(8):1853–62.
Article
PubMed
CAS
Google Scholar
Rick PD, Silver RP: Enterobacterial Common Antigen and capsular polysaccharides. In: Neidhardt FC, editor. Escherichia coli and Salmonella cellular and molecular biology. vol. 1, 2nd ed. Washington, DC: American Society of Microbiology; 1996. p. 104–22.
Reeves PR, Hobbs M, Valvano MA, Skurnik M, Whitfield C, Coplin D, Kido N, Klena J, Maskell D, Raetz CR, et al. Bacterial polysaccharide synthesis and gene nomenclature. Trends Microbiol. 1996;4(12):495–503.
Article
PubMed
CAS
Google Scholar
Malkamaki M. Antibodies to the enterobacterial common antigen: standardization of the passive hemagglutination test and levels in normal human sera. J Clin Microbiol. 1981;13(6):1074–9.
PubMed
PubMed Central
CAS
Google Scholar
Hubner I, Steinmetz I, Obst U, Giebel D, Bitter-Suermann D. Rapid determination of members of the family Enterobacteriaceae in drinking water by an immunological assay using a monoclonal antibody against enterobacterial common antigen. Appl Environ Microbiol. 1992;58(9):3187–91.
PubMed
PubMed Central
CAS
Google Scholar
Ito K, Iida M, Yamazaki M, Moriya K, Moroishi S, Yatsuyanagi J, Kurazono T, Hiruta N, Ratchtrachenchai O-A. Intimin types determined by heteroduplex mobility assay of Intimin gene (eae)-positive Escherichia coli strains. J Clin Microbiol. 2007;45(3):1038–41.
Article
PubMed
PubMed Central
CAS
Google Scholar
Adu-Bobie J, Frankel G, Bain C, Goncalves AG, Trabulsi LR, Douce G, Knutton S, Dougan G. Detection of intimins alpha, beta, gamma, and delta, four intimin derivatives expressed by attaching and effacing microbial pathogens. J Clin Microbiol. 1998;36(3):662–8.
PubMed
PubMed Central
CAS
Google Scholar
Oswald E, Schmidt H, Morabito S, Karch H, Marches O, Caprioli A. Typing of Intimin genes in human and animal enterohemorrhagic and enteropathogenic Escherichia coli: characterization of a new Intimin variant. Infect Immun. 2000;68(1):64–71.
Article
PubMed
PubMed Central
CAS
Google Scholar
Yu J, Kaper JB. Cloning and characterization of the eae gene of enterohaemorrhagic Escherichia coli O157:H7. Mol Microbiol. 1992;6(3):411–7.
Article
PubMed
CAS
Google Scholar
Elliott SJ, Wainwright LA, McDaniel TK, Jarvis KG, Deng YK, Lai LC, McNamara BP, Donnenberg MS, Kaper JB. The complete sequence of the locus of enterocyte effacement (LEE) from enteropathogenic Escherichia coli E2348/69. Mol Microbiol. 1998;28(1):1–4.
Article
PubMed
CAS
Google Scholar
Beutin L, Krause G, Zimmermann S, Kaulfuss S, Gleier K. Characterization of Shiga toxin-producing Escherichia coli strains isolated from human patients in Germany over a 3-year period. J Clin Microbiol. 2004;42(3):1099–108.
Article
PubMed
PubMed Central
Google Scholar
Ramachandran V, Hornitzky MA, Bettelheim KA, Walker MJ, Djordjevic SP. The common ovine Shiga toxin 2-containing Escherichia coli serotypes and human isolates of the same serotypes possess a Stx2d toxin type. J Clin Microbiol. 2001;39(5):1932–7.
Article
PubMed
PubMed Central
CAS
Google Scholar
Franke S, Harmsen D, Caprioli A, Pierard D, Wieler LH, Karch H. Clonal relatedness of Shiga-like toxin-producing Escherichia coli O101 strains of human and porcine origin. J Clin Microbiol. 1995;33(12):3174–8.
PubMed
PubMed Central
CAS
Google Scholar
Schmidt H, Scheef J, Morabito S, Caprioli A, Wieler LH, Karch H. A new Shiga toxin 2 variant (Stx2f) from Escherichia coli isolated from pigeons. Appl Environ Microbiol. 2000;66(3):1205–8.
Article
PubMed
PubMed Central
CAS
Google Scholar
Morabito S, Dell’Omo G, Agrimi U, Schmidt H, Karch H, Cheasty T, Caprioli A. Detection and characterization of Shiga toxin-producing Escherichia coli in feral pigeons. Vet Microbiol. 2001;82(3):275–83.
Article
PubMed
CAS
Google Scholar
Sonntag AK, Zenner E, Karch H, Bielaszewska M. Pigeons as a possible reservoir of Shiga toxin 2f-producing Escherichia coli pathogenic to humans. Berl Munch Tierarztl Wochenschr. 2005;118(11–12):464–70.
PubMed
CAS
Google Scholar
Baranzoni GM, Fratamico PM, Gangiredla J, Patel I, Bagi LK, Delannoy S, Fach P, Boccia F, Anastasio A, Pepe T. Characterization of Shiga toxin subtypes and virulence genes in porcine Shiga toxin-producing Escherichia coli. Front Microbiol. 2016;7:574.
Article
PubMed
PubMed Central
Google Scholar
Mellmann A, Bielaszewska M, Zimmerhackl LB, Prager R, Harmsen D, Tschape H, Karch H. Enterohemorrhagic Escherichia coli in human infection: in vivo evolution of a bacterial pathogen. Clin Infect Dis. 2005;41(6):785–92.
Article
PubMed
Google Scholar
Bielaszewska M, Prager R, Köck R, Mellmann A, Zhang W, Tschäpe H, Tarr PI, Karch H. Shiga toxin gene loss and transfer in vitro and in vivo during enterohemorrhagic Escherichia coli O26 infection in humans. Appl Environ Microbiol. 2007;73(10):3144–50.
Article
PubMed
PubMed Central
CAS
Google Scholar
Fach P, Perelle S, Dilasser F, Grout J. Comparison between a PCR-ELISA test and the vero cell assay for detecting Shiga toxin-producing Escherichia coli in dairy products and characterization of virulence traits of the isolated strains. J Appl Microbiol. 2001;90(5):809–18.
Article
PubMed
CAS
Google Scholar
Karch H, Meyer T. Single primer pair for amplifying segments of distinct Shiga-like-toxin genes by polymerase chain reaction. J Clin Microbiol. 1989;27(12):2751–7.
PubMed
PubMed Central
CAS
Google Scholar
Lin Z, Kurazono H, Yamasaki S, Takeda Y. Detection of various variant verotoxin genes in Escherichia coli by polymerase chain reaction. Microbiol Immunol. 1993;37(7):543–8.
Article
PubMed
CAS
Google Scholar
Paton AW, Paton JC, Goldwater PN, Manning PA. Direct detection of Escherichia coli Shiga-like toxin genes in primary fecal cultures by polymerase chain reaction. J Clin Microbiol. 1993;31(11):3063–7.
PubMed
PubMed Central
CAS
Google Scholar
Read SC, Clarke RC, Martin A, De Grandis SA, Hii J, McEwen S, Gyles CL. Polymerase chain reaction for detection of verocytotoxigenic Escherichia coli isolated from animal and food sources. Mol Cell Probes. 1992;6(2):153–61.
Article
PubMed
CAS
Google Scholar
Yamasaki S, Lin Z, Shirai H, Terai A, Oku Y, Ito H, Ohmura M, Karasawa T, Tsukamoto T, Kurazono H, et al. Typing of verotoxins by DNA colony hybridization with poly- and oligonucleotide probes, a bead-enzyme-linked immunosorbent assay, and polymerase chain reaction. Microbiol Immunol. 1996;40(5):345–52.
Article
PubMed
CAS
Google Scholar
Ge B, Zhao S, Hall R, Meng J. A PCR-ELISA for detecting Shiga toxin-producing Escherichia coli. Microbes Infect. 2002;4(3):285–90.
Article
PubMed
CAS
Google Scholar
Kimata K, Shima T, Shimizu M, Tanaka D, Isobe J, Gyobu Y, Watahiki M, Nagai Y. Rapid categorization of pathogenic Escherichia coli by multiplex PCR. Microbiol Immunol. 2005;49(6):485–92.
Article
PubMed
CAS
Google Scholar
Fratamico P, Strobaugh TP. Simultaneous detection of Salmonella spp. and Escherichia coli O157:H7 by multiplex PCR. J Ind Microbiol Biotechnol. 1998;21:92–8.
Article
CAS
Google Scholar
Osek J, Dacko J. Development of a PCR-based method for specific identification of genotypic markers of Shiga toxin-producing Escherichia coli strains. Zoonoses Public Health. 2001;48(10):771–8.
CAS
Google Scholar
Toma C, Lu Y, Higa N, Nakasone N, Chinen I, Baschkier A, Rivas M, Iwanaga M. Multiplex PCR assay for identification of human diarrheagenic Escherichia coli. J Clin Microbiol. 2003;41(6):2669.
Article
PubMed
PubMed Central
CAS
Google Scholar
Hervert CJ, Martin NH, Boor KJ, Wiedmann M. Survival and detection of coliforms, Enterobacteriaceae, and gram-negative bacteria in Greek yogurt. J Dairy Sci. 2017;100(2):950–60.
Article
PubMed
CAS
Google Scholar