- Research note
- Open Access
Association between cesarean section and constipation in infants: the Japan Environment and Children’s Study (JECS)
BMC Research Notes volume 11, Article number: 882 (2018)
There have been increasing reports on the association between cesarean section (C-section) and the subsequent development of diseases in infants. C-section affects the diversity of microbiota in the infant’s gut. In the present study, we investigated the association between infants delivered by C-section and the development of constipation at 1 year old due to altered gut microbiota using data from the Japan Environment and Children’s Study (JECS).
This cohort study (n = 83,019) used data from JECS, an ongoing cohort study which began in January 2011. Data on bowel movement and potential confounding factors were recorded. A log-binomial regression model was used to estimate the risk of C-section, and the results were expressed as risk ratios and their respective 95% confidence intervals. Although infants delivered by C-section were of significantly younger gestational age and lesser birth weight than vaginally delivered infants, the frequency of bowel movements was almost similar between the two, independent of the mode of delivery. The prevalence of constipation in the entire infant was 1.37%. No significant differences were observed for C-section in crude and adjusted risk ratios for constipation.
During the last few decades, there have been increasing number of reports on the association between cesarean section (C-section) and the development of diseases later in childhood. C-section has been associated with several diseases in infants, such as asthma, gastroenteritis, inflammatory bowel disease, celiac disease, type 1 diabetes mellitus, and obesity [1,2,3,4,5]. Because infants born by C-section are not exposed to the mother’s vaginal and intestinal microbiota, the microbiota in these infants is similar to the mother’s skin flora . In mice, the first exposure to microorganisms seems to be important for gut microbiota and priming of the regulatory immune system . Thus, the mode of delivery may be a crucial factor for infants in terms of the development of the immune system and subsequent incidence of disease.
Functional gastrointestinal disorders, such as infantile colic, gastroesophageal reflux, and constipation, may be caused by abnormal intestinal flora . Disruption of the intestinal microbiota is considered an etiological factor in pediatric functional constipation . We therefore hypothesized that infants delivered by C-section could show increased morbidity to constipation due to altered gut microbiota.
In the present study, we investigated the association between infants delivered by C-section and the development of constipation using data from the Japan Environment and Children’s Study (JECS).
In this study, data were obtained from JECS, an ongoing birth cohort study that commenced in January 2011. JECS was designed to investigate mothers and children using surveys until their newborns reached the age of 13 years. It is scheduled to continue up to 2027 and is measuring the effect of environmental factors on children’s health. The detailed methodology has been previously reported .
Briefly, pregnant women from 15 regions in Japan were recruited for 3 years from 2011 to 2014. The JECS protocol was approved by the Review Board on Epidemiological Studies of the Ministry of the Environment and by the Ethics Committees of all participating institutions. JECS was conducted after obtaining written informed consent from all participants. A total of 103,062 pregnancies have participated in JECS (set jecs-an-20180131). After excluding multiple participation, multiple births, and miscarriages or still births, the remaining 92,790 women had singleton live births. Missing information about the mode of delivery or the infants’ defecation habit resulted in the exclusion of 9771 women; consequently, 83,019 participants were analyzed in the present study.
Follow-up was accomplished with self-administered questionnaires, which were completed at 1 month, 6 month and 1 year after birth. The questionnaires collected data related to the breastfeeding, stool condition, and confounding factors. Medical data were collected from the transcribed medical records and included gestational age, birth weight, mode of delivery and maternal age. The rate of exclusive breastfeeding was determined at 1 month after birth. Constipation was defined as chronic fecal retention characterized by defecation frequency of < 3 per week at 1 year old.
The correlation between C-section and constipation in infants was assessed. A log-binomial regression model was used to estimate the crude risk, confounding-adjusted risk, and 95% confidence interval (CI) for C-section-delivered infants. The following potential factors were assessed for confounding-adjusted risk: Adjusted for maternal age, marital status, body mass index before pregnancy, employment status, total income, education, maternal allergy, exercise and total calorie intake during pregnancy, smoking and alcohol consumption after 1 month delivery, previous delivery, gestational age, birth weight, sex of the neonate, congenital anomaly of the neonate, passive smoking and feeding at 1 month birth, and nursing at 6 months. All statistical analyses were performed using SAS version 9.4 (SAS Institute Inc., Cary, NC, USA).
Clinical characteristics of participants are shown in Table 1. C-section-delivered infants had younger gestational age and lesser birth weight than those of vaginally delivered infants (p < 0.001). The rate of exclusive breastfeeding was lower and maternal age was higher in the C-section group than in the vaginal delivery group (p < 0.001). The frequency of bowel movements is shown in Table 2. There was no difference between the groups at 1 year old. The prevalence of infant constipation in the entire cohort was 1.37%. Crude and adjusted risk ratios for constipation in C-section-delivered infants are shown in Table 3. No significant differences were observed between the two groups at 1 year old.
In the present study, C-section was not associated with constipation in infants. This is the first report which addresses the direct association between cesarean section and constipation in infants. Because infants born by C-section have a less diverse gut flora [6, 7], we expected that they would tend to show constipation. However, the frequency of bowel movement was the same for both the groups (Table 2). Most instances of constipation in infants occurred at the time of transition from breastfeeding to consumption of infant formula . Mothers who delivered by C-section had lower rates of exclusive breastfeeding than those who delivered vaginally (Table 1). Bowel movements were analyzed by excluding the mode of feeding (breastfeeding or infant formula) to demonstrate the effect of the mode of delivery.
Although there are several definitions of constipation, bowel movement of < 3 per week is acceptable in infants . In the present study, the rate of constipation (< 3 week) was 1.37%, which is considerably lower than Western countries (Italy, 17.6% ; USA, 5% ). The prevalence of infants’ constipation has been reported range 0.05% to 0.3% in Thailand which is low rate like Japan . The large variation in prevalence of constipation may be due to different country and food culture. This low rate of constipation in Japanese infants may have resulted in no differences between the groups in present study.
This study showed that C-section has no effect on the frequency of bowel movement in infants at 1 year old.
Our study had several limitations. First, there were differences in the backgrounds of the two groups (Table 1). C-section-delivered infants were younger and had lesser birth weight than vaginally delivery infants, which may have resulted in differences in bowel maturation. However, Iacono et al. found that preterm birth is not associated with constipation . Second, regarding the timing of analysis, there is a wide range of age with respect to the onset of constipation . A diagnosis of constipation was made at 1 year of age in this study. Most studies evaluate the prevalence of constipation in children between 2 and 14 years of age [15,16,17]. The onset of constipation occurs within the first year of life in almost 50% of the affected children . To investigate the effect of C-section on constipation, further studies will be required to determine the prevalence of constipation across different age groups.
Japan Environment and Children’s Study
Kuhle S, Tong OS, Woolcott CG. Association between caesarean section and childhood obesity: a systematic review and meta-analysis. Obes Rev. 2015;16:295–303.
Kristensen K, Henriksen L. Cesarean section and disease associated with immune function. J Allergy Clin Immunol. 2016;137:587–90.
Bager P, Simonsen J, Nielsen NM, Frisch M. Cesarean section and offspring’s risk of inflammatory bowel disease: a national cohort study. Inflamm Bowel Dis. 2012;18:857–62.
Decker E, Engelmann G, Findeisen A, Gerner P, Laass M, Ney D, et al. Cesarean delivery is associated with celiac disease but not inflammatory bowel disease in children. Pediatrics. 2010;125:e1433–40.
Cardwell CR, Stene LC, Joner G, Cinek O, Svensson J, Goldacre MJ, et al. Caesarean section is associated with an increased risk of childhood-onset type 1 diabetes mellitus: a meta-analysis of observational studies. Diabetologia. 2008;51:726–35.
Dominguez-Bello MG, Costello EK, Contreras M, Magris M, Hidalgo G, Fierer N, et al. Delivery mode shapes the acquisition and structure of the initial microbiota across multiple body habitats in newborns. Proc Natl Acad Sci USA. 2010;107:11971–5.
Hansen CH, Andersen LS, Krych L, Metzdorff SB, Hasselby JP, Skov S, et al. Mode of delivery shapes gut colonization pattern and modulates regulatory immunity in mice. J Immunol. 2014;193:1213–22.
Indrio F, Di Mauro A, Di Mauro A, Riezzo G, Panza R, Cavallo L, et al. Prevention of functional gastrointestinal disorders in neonates: clinical and socioeconomic impact. Benef Microbes. 2015;6:195–8.
de Meij TG, de Groot EF, Eck A, Budding AE, Kneepkens CM, Benninga MA, et al. Characterization of microbiota in children with chronic functional constipation. PLoS ONE. 2016;11:e0164731.
Kawamoto T, Nitta H, Murata K, Toda E, Tsukamoto N, Hasegawa M, et al. Rationale and study design of the Japan environment and children’s study (JECS). BMC Public Health. 2014;14:25.
Iacono G, Merolla R, D’Amico D, Bonci E, Cavataio F, Di Prima L, et al. Gastrointestinal symptoms in infancy: a population-based prospective study. Dig Liver Dis. 2005;37:432–8.
van den Berg MM, Benninga MA, Di Lorenzo C. Epidemiology of childhood constipation: a systematic review. Am J Gastroenterol. 2006;101:2401–9.
van Tilburg MA, Hyman PE, Walker L, Rouster A, Palsson OS, Kim SM, et al. Prevalence of functional gastrointestinal disorders in infants and toddlers. J Pediatr. 2015;166:684–9.
Osatakul S, Puetpaiboon A. Use of Rome II versus Rome III criteria for diagnosis of functional constipation in young children. Pediatr Int. 2014;56:83–8.
Yong D, Beattie RM. Normal bowel habit and prevalence of constipation in primary-school children. Ambul Child Health. 1998;4:277–82.
Kajiwara M, Inoue K, Usui A, Kurihara M, Usui T. The micturition habits and prevalence of daytime urinary incontinence in Japanese primary school children. J Urol. 2004;171:403–7.
Issenman RM, Hewson S, Pirhonen D, Taylor W, Tirosh A. Are chronic digestive complaints the result of abnormal dietary patterns? Diet and digestive complaints in children at 22 and 40 months of age. Am J Dis Child. 1987;141:679–82.
Del Ciampo IR, Galvão LC, Del Ciampo LA, Fernandes MI. Prevalence of chronic constipation in children at a primary health care unit. J Pediatr. 2002;78:497–502.
TY, KM, HI and AT designed the study. KM, KH and AT analyzed and interpreted the data. TY KM, and AT wrote the manuscript. All authors contributed to the critical revision of the manuscript. All authors read and approved the final manuscript.
We would like to express our gratitude to all participants of this study, and all individuals involved in data collection.
The authors declare that they have no competing interests.
Availability of data and materials
The data used to derive our conclusions are unsuitable for public deposition due to ethical restrictions and specific legal framework in Japan. The Ethical Guidelines for Epidemiological Research enforced by the Japan Ministry of Education, Culture, Sports, Science, and Technology and the Ministry of Health, Labor and Welfare also restricts the open sharing of epidemiological data.
Consent for publication
Ethics approval and consent to participate
The JECS protocol was approved by the Review Board of the Ministry of the Environment for epidemiological studies, and by the Ethics Committees of all participating institutions. The JECS is conducted in accordance with the Declaration of Helsinki and other nationally valid regulations, and written informed consent was obtained from all participants.
JECS was funded by the Ministry of the Environment, Japan. The findings and conclusions of this article are solely the responsibility of the authors and do not represent the official views of government. The funding bodies had no role in the study design, collection and analysis of data, interpretation of results, writing of the manuscript, or decision to publish.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Members of the JECS (principal investigator, Toshihiro Kawamoto) as of 2018: Yukihiro Ohya (National Center for Child Health and Development, Tokyo, Japan), Reiko Kishi (Hokkaido University, Sapporo, Japan), Nobuo Yaegashi (Tohoku University, Sendai, Japan), Koichi Hashimoto (Fukushima Medical University, Fukushima, Japan), Chisato Mori (Chiba University, Chiba, Japan), Shuichi Ito (Yokohama City University, Yokohama, Japan), Zentaro Yamagata (University of Yamanashi, Chuo, Japan), Hidekuni Inadera (University of Toyama, Toyama, Japan), Michihiro Kamijima (Nagoya City University, Nagoya, Japan), Takeo Nakayama (Kyoto University, Kyoto, Japan), Hiroyasu Iso (Osaka University, Suita, Japan), Masayuki Shima (Hyogo College of Medicine, Nishinomiya, Japan), Yasuaki Hirooka (Tottori University, Yonago, Japan), Narufumi Suganuma (Kochi University, Nankoku, Japan), Koichi Kusuhara (University of Occupational and Environmental Health, Kitakyushu, Japan), and Takahiko Katoh (Kumamoto University, Kumamoto, Japan).
About this article
Cite this article
Yoshida, T., Matsumura, K., Tsuchida, A. et al. Association between cesarean section and constipation in infants: the Japan Environment and Children’s Study (JECS). BMC Res Notes 11, 882 (2018) doi:10.1186/s13104-018-3990-5
- Birth cohort
- Cesarean section