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Comparison and impact of associated anomalies on the anal position index in neonates with anorectal malformation

Abstract

Objective

Some prognostic factors have affected the functional outcomes of patients with anorectal malformations (ARM) after definitive surgery, including the associated anomalies. Moreover, the anal position index (API) study in neonates from developing countries is minimal. We aimed to (1) compare the API between neonates with ARM and controls; and (2) determine the impact of associated anomalies on the API in neonates with ARM.

Results

We ascertained 68 subjects: 35 neonates with ARM and 33 controls. The API of neonates with ARM was similar to controls, either male or female neonates (p = 0.51 and 0.90, respectively). Interestingly, the API in ARM males with associated anomalies (0.42 ± 0.07) was significantly lower than in control males (0.48 ± 0.02) (p = 0.005). Moreover, the API of ARM neonates with vertebral anomalies (0.35 ± 0.04) was lower than ARM neonates without vertebral anomalies (0.47 ± 0.07) (p = 0.021). In conclusion, associated anomalies and sex might affect the API in neonates with ARM. These findings should be considered and informed during counseling to the parents regarding the prognosis of functional outcomes in ARM neonates, particularly with associated anomalies.

Introduction

Anal position index (API) is a quantitative measurement to determine the normal anal position by defining the ratio of scrotum–anal distance to scrotum-coccyx distance and fourchette-anal distance to fourchette–coccyx distance for males and females, respectively [1].

Some prognostic factors have affected the functional outcomes of patients with anorectal malformations (ARM) after definitive surgery, including the associated anomalies [2,3,4,5]. The associated anomalies found in ARM are genitourinary (40–50%), followed by cardiovascular (30–35%), spinal cord tethering (25–30%), gastrointestinal anomalies (5–10%), and VACTERL (4–9%) anomalies [2]. Moreover, the API study in neonates from developing countries is minimal [6, 7]. Here, we aimed: (1) to compare the API between neonates with ARM and controls; and (2) to determine the impact of associated anomalies on the API in neonates with ARM.

Main text

Material and methods

Patient samples

We evaluated the medical records of neonates with ARM and controls at our institution from November 2018 and April 2021. Neonates with incomplete medical records were excluded. The Institutional Review Board of the Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada/Dr. Sardjito Hospital (KE/FK/1191/EC/2020) approved this study. Written informed consent was obtained from the parents or guardians of neonates.

Anal position index (API)

For controls, API was determined as the ratio of scrotum–anal distance to scrotum-coccyx distance and of fourchette-anal distance to fourchette–coccyx distance for males and females, respectively. For neonates with ARM, API was defined as the ratio of scrotum—the center of anal dimple distance to scrotum-coccyx distance and fourchette-center of anal dimple distance to fourchette–coccyx distance for males and females, respectively (Additional file 1: Fig. S1). API was determined prior to surgery at the time of identification of an ARM at birth. Anal dimple is an area of usually elevated, hyperpigmented skin in the perineum midline in neonates with ARM. However, its epicenter might be depressed (anal fossette) [8].

Statistical analysis

The data were presented as frequency (percentage) and mean ± SD. The significance of mean differences among groups was determined using the One-way Anova test. The p-value of < 0.05 was considered statistically significant. The IBM Statistical Package for Social Science (SPSS) version 21 (IBM Corp., Chicago) was used to perform all statistical analyses.

Results

Baseline characteristics of neonates

We ascertained 68 neonates: 35 neonates with ARM, consisting of 30 males and five females, and 33 controls, involving 17 males and 16 females. Most associated anomalies in ARM neonates were congenital heart disorder (63.6%), followed by Down syndrome (54.5%), vertebral anomaly (27.3%), trachea-oesophageal anomaly (27.3%), and limb anomaly (18.2%). The frequency of associated anomalies was a significant difference between male and female neonates (p = 0.005) (Additional file 2: Table S1) [9].

Comparison of API between neonates with ARM and controls

The API of neonates with ARM was similar to controls, either male or female neonates (p = 0.51 and 0.90, respectively) (Table 1) [9].

Table 1 Comparison of API between neonates with ARM and controls according to sex

Next, we conducted a subgroup analysis of API differences of neonates with associated anomalies and controls stratified by subjects’ sex. (Table 2). The API of ARM males with associated anomalies (0.42 ± 0.07) was significantly lower than control males (0.48 ± 0.02) (p = 0.005) (Table 2).

Table 2 Comparison of API between ARM neonates with associated anomalies and controls stratified by subjects’ sex

Association of API and associated anomalies in neonates with ARM

The API of ARM neonates with vertebral anomalies (0.35 ± 0.04) was lower than ARM neonates without vertebral anomalies (0.47 ± 0.07) (p = 0.021), while the other associated anomalies were not correlated with the API (p > 0.05) (Table 3).

Table 3 Association of API and associated anomalies in neonates with ARM

Discussion

Here, our study is able to provide new data on API in neonates, both controls and ARM from a developing country and different populations from previous reports [6, 7, 10,11,12,13,14]. We included the ARM patients with associated anomalies, including the vertebral anomaly, as the novelty of our study (vs. a normal sacrum was the inclusion criteria [12]). In addition, there are still variations in the API among studies [14]. These API variations might be due to different methods for API measurement and ethnic populations [15]. The measurement of API is suggested to determine the neonates' age to minimize the impact of ethnic variations on the API. However, a recent systematic review concluded that ethnic variations did not affect the API [8].

Our study shows that the API is affected by the associated anomalies in neonates with ARM, particularly in males. The associated anomalies have been associated with the functional outcomes of ARM patients after definitive surgery [2,3,4,5]. Therefore, it is suggested that a pediatric surgeon find any associated anomalies in neonates with ARM to ensure appropriate management and counseling for the parents [16].

Another novelty of our study is that we included all ARM neonates with and without associated anomaly vs. ARM neonates without sacral anomaly [12]. In addition, they suggested not using the anal dimple as the proposed neoanus since it might be anterior to the normal anus position [12]. A previous report also suggested that the API should not be used as the only parameter for surgical intervention [13] because the API measurement might be inaccurate, particularly in patients with constipation. Constipation might result in perineal elongation due to the fecal impaction in the rectum [8]. The association between API and constipation is controversial. While some studies noted their association [10, 16], other reports were not [7, 15, 18]. Notably, our study aimed to compare the API between neonates with ARM and controls and determine the impact of associated anomalies on the API in neonates with ARM. Therefore, we did not associate the API with ARM patients' prognosis after definitive surgery. Our study focused on the associated anomalies in ARM patients that affected the API.

Interestingly, our subgroup analysis revealed that the API in ARM males with associated anomalies was significantly lower than in control males (Table 2). Our study is the first report that analyzed the impact of sex and associated anomalies in ARM neonates on the API to the best of our knowledge. In addition, although it was not statistically significant, the ARM group with associated anomalies also had a lower birth weight. Therefore, studying the association between API and birth weight is interesting.

Among associated anomalies, only vertebral anomaly showed a significant association with the API, revealing that ARM neonates with vertebral anomaly have lower API than ARM neonates without vertebral anomaly (Table 3). These findings were also another novelty of our study. However, we did not determine the sacral ratio in our patients. It is known that patients with a lower sacral ratio may have an influence on the API. Furthermore, the most common associated anomalies in our patients were congenital heart disorder (63.6%), followed by Down syndrome (54.5%), vertebral (27.3%), trachea-oesophageal (27.3%), and limb anomaly (18.2%). In contrast, a previous study showed that the most common associated anomalies in ARM patients were genitourinary (39.7%), spinal anomaly (33.3%), and congenital heart disorder (16.1%) [19].

Conclusions

Associated anomalies and sex might affect the API in neonates with ARM. These findings should be considered and informed during counseling to the parents regarding the prognosis of functional outcomes in ARM neonates, particularly with associated anomalies.

Limitations

Several limitations of our study are as follows: (1) we included the neonates with ARM and controls from one institution only. It might not reflect all ethnic populations in Indonesia; (2) limited sample size. A multicentre study with a larger sample size is essential to clarify our findings; (3) we classified several vertebral anomalies within one group for analysis; (4) we did not associate the API and the location of the fistula. It is known that the higher the malformation, the closer the anal sphincter is located. Moreover, there are more associated anomalies in the ARM patients with a higher fistula location. These facts should be noted during the interpretation of our results.

Availability of data and materials

All data generated or analyzed during this study are included in the submission. The raw data are available from the corresponding author on reasonable request.

Abbreviations

API:

Anal position index

ARM:

Anorectal malformation

SD:

Standard deviation

References

  1. Reisner SH, Sivan Y, Nitzan M, Merlob P. Determination of anterior displacement of the anus in newborn infants and children. Pediatrics. 1984;73:216–7.

    Article  CAS  Google Scholar 

  2. Makrufardi F, Arifin DN, Afandy D, Yulianda D, Dwihantoro A, Gunadi. Anorectal malformation patients’ outcomes after definitive surgery using Krickenbeck classification: a cross-sectional study. Heliyon. 2020;6:e03435.

    Article  PubMed Central  Google Scholar 

  3. Rahmi MA, Ryantono F, Maharani A, Dwihantoro A, Gunadi. Functional outcomes in anorectal malformation patients following definitive surgery. Mal J Med Health Sci. 2020;16:1–5.

    Google Scholar 

  4. Wood RJ, Levitt MA. Anorectal malformations. Clin Colon Rectal Surg. 2018;31:61–70.

    Article  PubMed Central  Google Scholar 

  5. Levitt MA, Kant A, Pena A. The morbidity of constipation in patients with anorectal malformations. J Pediatr Surg. 2010;45:1228–33.

    Article  Google Scholar 

  6. Sharma S, Perumal V, Sharma K, Gupta DK. Varied parameters and utility of the anal position index: a systematic review and meta-analysis. Pediatr Surg Int. 2021;37:469–77.

    Article  Google Scholar 

  7. Duci M, Fascetti-Leon F, Bogana G, Gamba P, Midrio P. Conservative management of anterior located anus: a medium-long term follow up. J Pediatr Surg. 2021;S0022–3468(21):00297–9.

    Google Scholar 

  8. Davies MRQ, Rode H. Recent advances concerning the normal and abnormal anatomy of the anus and rectum. In: Holschneider AM, Hutson JM, editors. Anorectal malformations in children. Heidelberg: Springer; 2006. p. 65–86.

    Google Scholar 

  9. Pitaka RT Makhmudi A Gunadi. Perbandingan Indeks Posisi Anus Antara Neonatus Dengan Dan Tanpa Malfrmasi Anorektal (Bahasa) Universitas Gadjah Mada. Thesis (Unpublished document). 2021.

  10. Erturk N, Kandemir O. Determination of normal anal position index using a modified technique in Turkish neonates. J Indian Assoc Pediatr Surg. 2017;22:29–32.

    Article  PubMed Central  Google Scholar 

  11. Núñez-Ramos R, Fabbro MA, González-Velasco M, Núñez Núñez R, Romanato B, Vecchiato L, et al. Determination of the anal position in newborns and in children with chronic constipation: comparative study in two European healthcare centres. Pediatr Surg Int. 2011;27:1111–5.

    Article  Google Scholar 

  12. Patel JN, Kumar A, Yadav PS, Chadha R, Datta V, Roy CS. The position of the anal dimple in newborns and infants with anorectal malformations and its correlation with the normal anal position. J Pediatr Surg. 2018;53:1560–5.

    Article  Google Scholar 

  13. Rerksuppaphol S, Rerksuppaphol L. Normal anal position in Thai newborns. J Med Assoc Thai. 2008;91:1839–44.

    PubMed  Google Scholar 

  14. Chan WT, Lee HC, Wang WN, et al. Determination of the normal position of the anus in Taiwanese infants. Pediatr Neonatol. 2009;50:158–61.

    Article  Google Scholar 

  15. Herek O, Polat A. Incidence of anterior displacement of the anus and its relationship to constipation in children. Surg Today. 2004;34:190–2.

    Article  Google Scholar 

  16. Cho S, Moore SP, Fangman T. One hundred three consecutive patients with anorectal malformations and their associated anomalies. Arc Arch Pediatr Adolesc. 2001;155:587–91.

    Article  CAS  Google Scholar 

  17. Thambidorai CR, Raghu R, Zulfiqar A. Magnetic resonance imaging in anterior ectopic anus. Pediatr Surg Int. 2008;24:161–5.

    Article  CAS  Google Scholar 

  18. Kyrklund K, Pakarinen MP, Taskinen S, Rintala RJ. Bowel function and lower urinary tract symptoms in females with anterior anus treated conservatively: controlled outcomes into adulthood. J Pediatr Surg. 2015;50:1168–73.

    Article  Google Scholar 

  19. Totonelli G, Catania VD, Morini F, Fusaro F, Mosiello G, Iacobelli BD, Bagolan P. VACTERL association in anorectal malformation: effect on the outcome. Ped Surg Int. 2015;31:805–8.

    Article  Google Scholar 

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Acknowledgements

We would like to express our gratitude to all who assisted us with the research professionally. Some results for the manuscript are from Ririd Tri Pitaka’s thesis.

Funding

This study was funded by the Universitas Gadjah Mada.

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Authors and Affiliations

Authors

Contributions

RTP, AM and G conceived the study. G and ARF drafted the manuscript, and AM critically revised the manuscript for important intellectual content. RTP, G, ARF, and AM facilitated all project-related tasks. All authors have read and approved the manuscript and agreed to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved. All author read and approved the final manuscript.

Corresponding author

Correspondence to Gunadi.

Ethics declarations

Ethics approval and consent to participate

This study was approved by the Medical and Health Research Ethics Committee of Universitas Gadjah Mada's Faculty of Medicine, Public Health, and Nursing/Dr. Sardjito Hospital (KE/FK/1191/EC/2020). Written informed consent was obtained from the patients’ parents for participating in this study.

Consent to publication

Written informed consent was taken from the patient’s parents for publication of the associated images.

Competing interests

The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

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Supplementary Information

Additional file1: Figure S1

. API measurement in controls: API was determined as the ratio of scrotum–anal distance to scrotum-coccyx distance and of fourchette-anal distance to fourchette–coccyx distance for males a and females b, respectively; and neonates with ARM: API was defined as the ratio of scrotum–center of anal dimple distance to scrotum-coccyx distance and fourchette-center of anal dimple distance to fourchette–coccyx distance for males c and females d, respectively.

Additional file2:

Table S1. Baseline characteristics of neonates in our study.

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Pitaka, R.T., Fauzi, A.R., Makhmudi, A. et al. Comparison and impact of associated anomalies on the anal position index in neonates with anorectal malformation. BMC Res Notes 15, 294 (2022). https://doi.org/10.1186/s13104-022-06186-x

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