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Expression of the transcription factor, TFII-I, during post-implantation mouse embryonic development
BMC Research Notes volume 3, Article number: 203 (2010)
General transcription factor (TFII-I) is a multi-functional transcription factor encoded by the Gtf2i gene, that has been demonstrated to regulate transcription of genes critical for development. Because of the broad range of genes regulated by TFII-I as well as its potential role in a significant neuro-developmental disorder, developing a comprehensive expression profile is critical to the study of this transcription factor. We sought to define the timing and pattern of expression of TFII-I in post-implantation embryos at a time during which many putative TFII-I target genes are expressed.
Antibodies to the N-terminus of TFII-I were used to probe embryonic mouse sections. TFII-I protein is widely expressed in the developing embryo. TFII-I is expressed throughout the period from E8-E16. However, within this period there are striking shifts in localization from cytoplasmic predominant to nuclear. TFII-I expression varies in both a spatial and temporal fashion. There is extensive expression in neural precursors at E8. This expression persists at later stages. TFII-I is expressed in developing lung, heart and gut structures. There is no evidence of isoform specific expression. Available data regarding expression patterns at both an RNA and protein level throughout development are also comprehensively reviewed.
Our immunohistochemical studies of the temporal and spatial expression patterns of TFII-I in mouse embryonic sections are consistent with the hypothesis that hemizygous deletion of GTF2I in individuals with Williams-Beuren Syndrome contributes to the distinct cognitive and physiological symptoms associated with the disorder.
TFII-I, or General Transcription Factor II-I (GTF2-I) is a member of ubiquitously expressed, multifunctional transcription factor family that integrates signals from multiple pathways and mediates cellular response to changes in the external environment . Both the high degree of sequence conservation in TFII-I among species and the lack of individuals with homozygous deletion of GTF2I suggest that its ubiquitous expression and various molecular functions are essential for viability.
TFII-I gene (Entrez Gene ID 2969) has been mapped to an interval of the human chromosome 7q11.23 (chr7:73,805,362-73,812,956). This region is commonly deleted in Williams-Beuren syndrome (WBS) (OMIM#194050), which is typically associated with hemizygous microdeletion of a 1.6 Mb region containing about 16 genes [2–5]. In mouse, the gene (Entrez Gene ID 14886, MGI 1202722) is located on chromosome 5qG2 (chr5:134,713,704-134,790,616). Experimentally generated mutant mice heterozygotic for Gtf2i and for its related transcription factor Gtf21-rd1 featured anomalies similar to those observed in Williams-Beuren Syndrome: retarded growth, microcephaly and craniofacial and skeletal defects. Homozygous loss of Gtf2i caused embryonic lethality .
Structurally, the TFII-I protein comprises several domains that define its biological function. Additional File 1 (Structure, Functions and Cellular Localization of TFII-I) presents schematic diagram of TFII-I protein structure and discusses functions of the domains. So far, direct involvement of TFII-I in gene regulation has been confirmed for more than 20 genes. Additional File 2 (TFII-I Target Genes) presents a list of genes that contain TFII-I binding sites in their sequences and that were found controlled by TFII-I.
In order to expand on the available description of timing and location of the expression of TFII-I documented in the literature [2, 7, 8] we reviewed a number of resources on gene expression profile. Additional File 3 (Developmental Expression of TFII-I mRNA and Reported Expression of TFII-I in Mouse Development) summarizes developmental expression of TFII-I mRNA based on profiles of expressed sequence tags (ESTs) available from NCBI , data from The Jackson Laboratory on Mouse Gene Expression Database [10, 11] and EMAGE--Edinburgh Mouse Atlas of Gene Expression .
We wished to expand on the existing data regarding expression with a focus on protein expression using immunohistochemistry to provide spatial detail. Detailed methods that were applied are described in Additional File 4 (Methods).
Expression of TFII-I by developmental stage
As suggested by previous studies, TFII-I immunoreactivity is present in mouse embryo continuously from E8-E16. The intensity, distribution and subcellular localization vary, dependent on the stage. At E8 (Figure 1A-D), TFII-I is expressed at higher levels in trophectodermal derivatives rather than in the embryo. Within the embryo TFII-I in the ectoderm is largely cytoplasmic. Expression is also present in the mesoderm, predominantly in a cytoplasmic distribution. In contrast, at E9, TFII-I shows discrete nuclear localization in neuroectoderm in addition to a more diffuse cytoplasmic distribution (Figure 2A-D and the inlet). Extensive TFII-I immunoreactivity is also present in the eye primordium (Figure 2B).
With increasing organ differentiation at E11, TFII-I can be localized to a number of developing organ systems (noted in detail below).
Sections through liver (Figure 3A) show widespread, low-level expression of TFII-I in developing hepatocytes. This immunoreactivity is fully blocked by pre-incubation of the antibody with peptide as described in methods (Figure 3C, D).
The expanding lung buds at E12 (Figure 4B) express high levels of TFII-I in the airways, vasculature and parenchyma. This pattern is demonstrated throughout embryonic development (Figure 4E, C) and is discussed in more detail below. In addition to expression in the pulmonary system, TFII-I is highly expressed in developing heart (Figure 4A). Expression in the brain is also noted (Figure 2E) and contrasts with that of the related transcription factor, TFII-IRD1 (Figure 2F), as we have previously described. TFII-I immunoreactivity is also noted in the developing nasopharynx (Figure 2G).
A survey of tissues from E15 demonstrates expression of TFII-I in multiple organ systems (Figures 3B, 4E, C, 5A-D). High levels of TFII-I expression are noted in skin and hair follicles (Figure 5A) as well as in submandibular glands (Figure 5B). Lung continues to show a similar pattern of expression to earlier time points (Figure 4E, C).
The relative expression of TFII-I in lung, diaphragm and liver is highlighted in Figure 3B with lung and diaphragm showing more abundant expression than liver. Again, immunoreactivity is eliminated by pre-incubation of the antibody with peptide (Figure 3C, D, 4D).
Similar patterns of expression are also seen at E16. Intense immunoreactivity is noted in skin (Figure 5C) as well as in subcutaneous vasculature (Figure 5A, B). Gut also demonstrates TFII-I expression, but at a lower level (Figure 5D). Lung continues to express TFII-I in the pattern seen at earlier stages (Figure 4C).
Expression of TFII-I by Organ Systems
As described above by developmental stage, TFII-I expression is widespread in developing mouse embryo. This, however, is not uniform, but instead shows variation in amount as well as sub-cellular distribution. Further, the expression appears to follow specific patterns based on organ system. These patterns are summarized in the sections below.
Expression in Vasculature, Heart and Lung
TFII-I protein has been detected in adult vasculature, endothelial cells, heart and lung. We examined the timing and localization of TFII-I in developing vascular structures and in heart as well as in lung. Expression was detected in vasculature beginning at E11 (data not shown). We were unable to identify vascular structures at earlier time points. In later time-points (Figure 4, 5), TFII-I immunoreactivity was noted in vascular endothelial cells in lung and dermis as well as in adjacent smooth muscle cells.
Heart showed high levels of TFII-I expression beginning at E11 (not shown) and throughout development (Figure 4A). Expression was uniform throughout the heart.
TFII-I immunoreactivity was noted throughout lung development. Staining was most intense in the airway epithelium early in development (Figure 4B). Later sections show staining in the adjacent vasculature as well as in the developing air sacs (Figure 4E, C). This pattern is also detected in adult mouse lung (not shown). The nasopharynx, the external extreme of the respiratory system, also expresses TFII-I from E12 (Figure 2G).
Expression of TFII-I in Gastrointestinal Tract and Liver
TFII-I expression is detected in the developing gut. Expression is most intense in the villi of the gut (Figure 5D). The liver also shows diffuse, low-level immunoreactivity as early as E11 (Figure 3A). This pattern is also noted at E15 (Figure 3B).
Expression of TFII-I in Glands and Skin
Significant TFII-I staining is noted in the skin and glands. Beginning at E11, skin and developing hair follicles show high levels of expression similar to those seen at E15 (Figure 5A). Significant TFII-I expression is also detected in the submandibular glands as noted at E15 (Figure 5B).
Expression of TFII-I in Central Nervous System
The presence of TFII-I has been examined both in development and adult brain because of its putative association with Williams-Beuren Syndrome . We find that TFII-I is expressed in a regionally defined fashion within the CNS consistent with previous reports (Figure 1 and 2). Further, we find that TFII-I and TFII-IRD1 demonstrate distinct patterns of expression in embryo (Figure 2E and 2F) as observed in adult mouse.
In early development only trace or low levels of Gtf2i were detected between 1-cell stage and E3.5. At day E3.5 strong signal in inner cell mass and moderate signal in trophoectoderm of blastocyst were revealed by direct immuno-fluorescence, RT-PCR and WISH (Whole Mount in Situ RNA Hybridization). For more details see Additional File 3.
Our studies have identified TFII-I protein expression in post-implantation embryos since the beginning of organogenesis, from E8 until E16. We found that in developmental period spanned by our study TFII-I was continuously present in the embryo, although it's spatial and temporal distribution varied, as well as its sub-cellular localization (see Table 1).
In adult animal TFII-I regulates expression of VEGFR-2 (Flk1, Kdr), a receptor critical in differentiation and angiogenesis [13, 14]. In mouse development Kdr can be detected as early as in E6.25 in embryo  and in E8.5 in extra-embryonic regions, in parallel to formation of blood islands . The fact that both genes are expressed at the same time may suggest that TFII-I control over Kdr launches early in embryonic life. It was shown recently that homozygous deletion of Gtf2i caused embryonic lethality due to defects in yolk sac vasculogenesis and angiogenesis: Gtf2i inactivation resulted in downregulation of the VEGFR2, which deteriorated vascular signaling .
We found high levels of TFII-I in the airway epithelium, starting at E11. This finding complements other study which showed expression of TFII-I in the epithelium and in the underlying mesenchyme during odontogenesis in mouse embryo, between day E12.5 and newborn .
Our data showing the presence of TFII-I in the embryo confirm its role in cell differentiation and organogenesis, although a mechanism of its interactions is not clear. In cultured cells TFII-I is capable of activating the cyclin D1 gene and thus inducing transition from phase G1 into phase S of the cell cycle . Cell entry into phase S, phase S progression and entry into phase G2/M were significantly delayed in TFII-I knocked down cells. It is postulated that in early phases of cell cycle TFII-I may also control PKC-β which additionally activates cyclin D1 via NFκB pathway. TFII-I itself can be phosphorylated by cyclin dependent kinase 1 (Cdk1) thus amplifying phase transition signals . First cyclins expressed in mouse embryo are cyclin b1 and b2, detected in the inner cell mass and the trophectoderm of the blastocyst at E4.5 , followed by cyclins D and E at E7.5 . Again, whether such an interaction between TFII-I and cyclins occurs at this early development stage, remains to be revealed.
Based on our observations and others [2, 6, 8, 17, 22–24] TFII-I is critical in early mouse development. The structure makes it uniquely capable of transducing multiple regulatory signals as occurs in development. Our data add to the existing knowledge on TFII-I function as a pleiotropic transcription factor.
cyclin dependent kinase 1
expressed sequence tag
General Transcription Factor II-I
matrix metalloproteinase 9: NGS: normal goat serum
phospholipase C gamma
Transcription Factor that shares similarity with TFII-I in the 95-amino-acid HLH-like I-repeat domain (other names: BEN/WBSCR11/MusTRD1/Cream1)
transient receptor potential calcium channel
Vascular endothelial growth factor receptor 2
whole mount in situ hybridization
Roy AL: Biochemistry and biology of the inducible multifunctional transcription factor TFII-I. Gene. 2001, 274: 1-13. 10.1016/S0378-1119(01)00625-4.
Jurado Perez LA, Wang YK, Peoples R, Coloma A, Cruces J, Francke U: A duplicated gene in the breakpoint regions of the 7q11.23 Williams-Beuren syndrome deletion encodes the initiator binding protein TFII-I and BAP-135, a phosphorylation target of BTK. Hum Mol Genet. 1998, 7: 325-34. 10.1093/hmg/7.3.325.
Ewart AK, Morris CA, Atkinson D, Jin W, Sternes K, Spallone P, Stock AD, Leppert M, Keating MT: Hemizygosity at the elastin locus in a developmental disorder, Williams syndrome. Nat Genet. 1993, 5: 11-16. 10.1038/ng0993-11.
Osborne LR, Li M, Pober B, Chitayat D, Bodurtha J, Mandel A, Costa T, Grebe T, Cox S, Tsui LC: A 1.5 million-base pair inversion polymorphism in families with Williams-Beuren syndrome. Nat Genet. 2001, 29: 321-5. 10.1038/ng753.
DeSilva U, Elnitski L, Idol JR, Doyle JL, Gan W, Thomas JW, Schwartz S, Dietrich NL, Beckstrom-Sternberg SM, McDowell JC: Generation and comparative analysis of approximately 3.3 Mb of mouse genomic sequence orthologous to the region of human chromosome 7q11.23 implicated in Williams syndrome. Genome Res. 2002, 12: 3-15. 10.1101/gr.214802.
Enkhmandakh B, Makeyev AV, Erdenechimeg L, Ruddle FH, Chimge NO, Tussie-Luna MI, Roy AL, Bayarsaihan D: Essential functions of the Williams-Beuren syndrome-associated TFII-I genes in embryonic development. Proc Natl Acad Sci USA. 2009, 106: 181-6. 10.1073/pnas.0811531106.
Danoff SK, Taylor HE, Blackshaw S, Desiderio S: TFII-I, a candidate gene for Williams syndrome cognitive profile: parallels between regional expression in mouse brain and human phenotype. Neuroscience. 2004, 123: 931-8. 10.1016/j.neuroscience.2003.08.038.
Enkhmandakh B, Bitchevaia N, Ruddle F, Bayarsaihan D: The early embryonic expression of TFII-I during mouse preimplantation development. Gene Expr Patterns. 2004, 4: 25-8. 10.1016/S1567-133X(03)00155-8.
Wheeler DL, Barrett T, Benson DA, Bryant SH, Canese K, Chetvernin V, Church DM, Dicuccio M, Edgar R, Federhen S: Database resources of the National Center for Biotechnology Information. Nucleic Acids Res. 2008, 36: D13-21. 10.1093/nar/gkm1000.
MGIWS Gene Expression Database (GXD), The Jackson Laboratory, Bar Harbor, Maine. 2005, In: Book. City, [http://www.informatics.jax.org]
Smith CM, Finger JH, Hayamizu TF, McCright IJ, Eppig JT, Kadin JA, Richardson JE, Ringwald M: The mouse Gene Expression Database (GXD): 2007 update. Nucleic Acids Res. 2007, 35: D618-23. 10.1093/nar/gkl1003.
Venkataraman S, Stevenson P, Yang Y, Richardson L, Burton N, Perry TP, Smith P, Baldock RA, Davidson DR, Christiansen JH: EMAGE--Edinburgh Mouse Atlas of Gene Expression: 2008 update. Nucleic Acids Res. 2008, 36: D860-5. 10.1093/nar/gkm938.
Jackson TA, Taylor HE, Sharma D, Desiderio S, Danoff SK: Vascular endothelial growth factor receptor-2: counter-regulation by the transcription factors, TFII-I and TFII-IRD1. J Biol Chem. 2005, 280: 29856-63. 10.1074/jbc.M500335200.
Mammoto A, Connor KM, Mammoto T, Yung CW, Huh D, Aderman CM, Mostoslavsky G, Smith LE, Ingber DE: A mechanosensitive transcriptional mechanism that controls angiogenesis. Nature. 2009, 457: 1103-8. 10.1038/nature07765.
Mitiku N, Baker JC: Genomic analysis of gastrulation and organogenesis in the mouse. Dev Cell. 2007, 13: 897-907. 10.1016/j.devcel.2007.10.004.
Damert A, Miquerol L, Gertsenstein M, Risau W, Nagy A: Insufficient VEGFA activity in yolk sac endoderm compromises haematopoietic and endothelial differentiation. Development. 2002, 129: 1881-92.
Ohazama A, Sharpe PT: TFII-I gene family during tooth development: candidate genes for tooth anomalies in Williams syndrome. Dev Dyn. 2007, 236: 2884-8. 10.1002/dvdy.21311.
Desgranges ZP, Ahn J, Lazebnik MB, Ashworth T, Lee C, Pestell RC, Rosenberg N, Prives C, Roy AL: Inhibition of TFII-I-dependent cell cycle regulation by p53. Mol Cell Biol. 2005, 25: 10940-52. 10.1128/MCB.25.24.10940-10952.2005.
Ashworth T, Roy AL: Phase specific functions of the transcription factor TFII-I during cell cycle. Cell Cycle. 2009, 8: 596-605. 10.4161/cc.8.4.7728.
Chapman DL, Wolgemuth DJ: Isolation of the murine cyclin B2 cDNA and characterization of the lineage and temporal specificity of expression of the B1 and B2 cyclins during oogenesis, spermatogenesis and early embryogenesis. Development. 1993, 118: 229-40.
Singer JD, Gurian-West M, Clurman B, Roberts JM: Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells. Genes Dev. 1999, 13: 2375-87. 10.1101/gad.13.18.2375.
Wang YK, Perez-Jurado LA, Francke U: A mouse single-copy gene, Gtf2i, the homolog of human GTF2I, that is duplicated in the Williams-Beuren syndrome deletion region. Genomics. 1998, 48: 163-170. 10.1006/geno.1997.5182.
Hinsley TA, Cunliffe P, Tipney HJ, Brass A, Tassabehji M: Comparison of TFII-I gene family members deleted in Williams-Beuren syndrome. Protein Sci. 2004, 13: 2588-99. 10.1110/ps.04747604.
Chimge NO, Mungunsukh O, Ruddle F, Bayarsaihan D: Gene expression analysis of TFII-I modulated genes in mouse embryonic fibroblasts. J Exp Zoolog B Mol Dev Evol. 2007, 308: 225-35. 10.1002/jez.b.21134.
Kaufman MH: The Atlas of Mouse Development. 1998, San Diego: Academic Press Limited, Revised Edition edn
We thank Drs. Steve Desiderio and Roger Reeves for helpful comments. We thank Dr. Harry Taylor for assistance in developing antibody reagents. We thank Joy Nicole Powell for assistance in compiling tables.
The authors declare that they have no competing interests.
IF analyzed immunohistochemistry and revised the manuscript. DS performed immunohistochemistry and assisted in the drafting of the manuscript. CB provided critical revisions to the manuscript. SD oversaw the project as a whole and drafted the manuscript.
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Fijalkowska, I., Sharma, D., Bult, C.J. et al. Expression of the transcription factor, TFII-I, during post-implantation mouse embryonic development. BMC Res Notes 3, 203 (2010). https://doi.org/10.1186/1756-0500-3-203
- Related Transcription Factor
- Phase Transition Signal
- Mouse Gene Expression
- Multifunctional Transcription Factor
- Diffuse Cytoplasmic Distribution