Inhibition of Streptococcus pneumoniae adherence to human epithelial cells in vitro by the probiotic Lactobacillus rhamnosus GG
- Sook-San Wong1, 2, 9,
- Zheng Quan Toh1, 2,
- Eileen M Dunne1,
- E Kim Mulholland1, 4, 5,
- Mimi LK Tang2, 6, 7,
- Roy M Robins-Browne3, 8,
- Paul V Licciardi†1, 2Email author and
- Catherine Satzke†1, 8
© Wong et al.; licensee BioMed Central Ltd. 2013
Received: 26 November 2012
Accepted: 1 April 2013
Published: 5 April 2013
Colonization of the nasopharynx by Streptococcus pneumoniae is considered a prerequisite for pneumococcal infections such as pneumonia and otitis media. Probiotic bacteria can influence disease outcomes through various mechanisms, including inhibition of pathogen colonization. Here, we examine the effect of the probiotic Lactobacillus rhamnosus GG (LGG) on S. pneumoniae colonization of human epithelial cells using an in vitro model. We investigated the effects of LGG administered before, at the same time as, or after the addition of S. pneumoniae on the adherence of four pneumococcal isolates.
LGG significantly inhibited the adherence of all the pneumococcal isolates tested. The magnitude of inhibition varied with LGG dose, time of administration, and the pneumococcal isolate used. Inhibition was most effective when a higher dose of LGG was administered prior to establishment of pneumococcal colonization. Mechanistic studies showed that LGG binds to epithelial cells but does not affect pneumococcal growth or viability. Administration of LGG did not lead to any significant changes in host cytokine responses.
These findings demonstrate that LGG can inhibit pneumococcal colonization of human epithelial cells in vitro and suggest that probiotics could be used clinically to prevent the establishment of pneumococcal carriage.
Streptococcus pneumoniae (the pneumococcus) is a Gram-positive bacterium that causes serious diseases such as pneumonia, meningitis and acute otitis media. Pneumococcal diseases are a leading cause of childhood morbidity and mortality worldwide, affecting more than three million children under the age of five, and causing an estimated 826,000 deaths in this age group each year . The disease burden is especially high in developing countries . Pneumococcal colonization of the nasopharynx is often asymptomatic, occurs early in life, and is considered a prerequisite for development of pneumococcal disease . In high-risk populations, pneumococci can colonize the nasopharynx within the first few weeks of life .
Pneumococcal conjugate vaccines (PCV) provide protection against the serotypes most prevalent in pediatric invasive disease . However, developing countries have a substantial burden of invasive disease from non-vaccine serotypes, and serotype replacement is likely to be more important in these settings . In addition, access to these vaccines is limited in resource-poor countries and colonization often occurs before the first dose of PCV, typically given at two months of age. Early life strategies to reduce or prevent colonization and carriage are urgently needed, particularly in populations with high rates of pneumococcal disease.
Probiotics are defined as “live microorganisms which when administered in adequate amounts confer a health benefit on the host” . They can influence host microbiota and play a role in disease prevention . Probiotics such as Lactobacillus and Bifidobacterium species are widely used in food products or as food supplements and have been extensively studied in the gastrointestinal tract . Probiotics are believed to benefit the host through several mechanisms including i) inhibition of colonization by pathogenic microorganisms , ii) modulation of host immune responses  and iii) improvement of epithelial cell barrier integrity .
Although less is known about the effects of probiotics in the respiratory tract, evidence that they could be used to prevent disease in this context is mounting [12, 13]. For example, lactobacilli have been shown to protect against pneumococcal infection in mice [14–16], and inhibit the invasion of group A streptococci in vitro. In humans, administration of a probiotic drink containing Lactobacillus rhamnosus GG (LGG), Bifidobacterium lactis sp B420, Lactobacillus acidophilus 145, and Streptococcus thermophilus reduced nasal colonization by Gram-positive pathogens in adults . These studies suggest that some probiotic species can reduce pneumococcal colonization, potentially serving as a safe and cost-effective complementary strategy to immunization. Here we describe the effects of the probiotic LGG on pneumococcal colonization using an in vitro adherence assay.
Optimization of the pneumococcal adherence assay
Pneumococcal isolates used in this study
PMP6 (ATCC 6305)
Adherence and invasion of pneumococcal isolates
234 ± 249
193 ± 14.6
48.1 ± 24.6
3.2 ± 2.1*
0.01 ± 0.009
43.8 ± 19.3
15.0 ± 20.3
0.9 ± 1.6
0.1 ± 0.2
3.1 ± 1.3
1.1 ± 0.2
0.01 ± 0.007
0.03 ± 0.03
0.6 ± 1.0
Effect of LGG on pneumococcal adherence
Effect of culture supernatants on pneumococcal adherence
LGG + Pnc
103.4 ± 10.3%
85.8 ± 1.6%
79.4 ± 26.5%
95.5 ± 12.0%
127.6 ± 40.7%
140.1 ± 25.6%
69.0 ± 43.0%
178.1 ± 26.9%
To assess whether the probiotic LGG could prevent colonization of S. pneumoniae in vitro, we tested its effect on the adherence of four pneumococcal isolates using an in vitro adherence assay. A fifth isolate, PMP41 (a serotype 3 strain), was excluded from the study due to low adherence. This was not unexpected given that serotype 3 is heavily encapsulated, which can result in low adherence . Results demonstrated that LGG inhibits pneumococcal adherence in vitro. Inhibition was most effective when LGG was added at a dose approximately 60-fold higher than pneumococci before pneumococcal adherence has been established.
Mechanistic studies demonstrated that LGG binds to epithelial cells, suggesting that LGG could prevent pneumococcal adherence either through steric hindrance or competition for binding sites. Heparin did not affect LGG adherence to epithelial cells, indicating that LGG does not compete for binding to glycosylaminoglycans. However, LGG could compete for binding to other molecules such as fibronectin and collagen, as both S. pneumoniae and Lactobacillus species have been shown to bind to these molecules [21–24]. Some probiotics are known to produce secreted compounds with antibacterial activity on other species [25, 26]. Co-culture experiments indicated that LGG does not have any direct effect on pneumococcal growth or viability, nor did secreted products present in culture media impact pneumococcal growth or adherence.
Several studies have reported increased secretion of inflammatory mediators IL-6 and IL-8 following exposure to pneumococci, both in vitro and in vivo[27–30]. LGG can modulate host cell production of IL-6 [31, 32] and IL-8 [33, 34] following exposure to pathogens or microbial antigens, such as flagellin, both in vivo and in vitro. Therefore, we hypothesized that exposure to LGG would reduce IL-6 and IL-8 production by epithelial cells. However, S. pneumoniae did not increase IL-8 or IL-6 secretion by epithelial cells, and other inflammatory cytokines and chemokines were undetectable. These data suggest that the inhibition of S. pneumoniae adherence to epithelial cells in this study was not due to LGG modulation of IL-6 or IL-8 production by epithelial cells. The lack of effect on cytokine secretion could be due to the relatively short incubation time or lack of co-stimulation. Marriott et al.  recently demonstrated that co-culture of pneumococci-primed macrophages, or supernatants from these cultures, with A549 epithelial cells significantly elevated IL-8 secretion, but not when pneumococcal bacteria were added to A549 cells alone, suggesting a critical role for epithelial-macrophage interactions in this response. Although not statistically significant, the reduction in IL-8 levels in the heparin-treated samples observed in our experiments (Figure 3C and 3D) is likely due to binding of IL-8 by heparin [36, 37].
Clinical studies investigating the impact of LGG administration on the incidence of otitis media and respiratory tract infections have reported variable outcomes. Rautava et al. found that infants who were given formula supplemented with LGG and Bifidobacterium lactis had a lower relative risk of otitis media and recurrent respiratory infections compared to infants given placebo . Administration of an LGG-containing probiotic mixture to women during the last month of pregnancy and to infants (who were given the probiotic mixture in combination with galactooligosaccharides) for six months after birth was associated with a reduction in infant respiratory infections, although no difference in otitis media was observed . Hosjak et al. found that LGG reduced the risk of respiratory and gastrointestinal infections in hospitalized children , whereas in children attending day care, LGG treatment reduced the relative risk of upper respiratory tract infections but did not affect lower respiratory tract or gastrointestinal tract infections . Few clinical data on the effects of probiotic treatment on nasopharyngeal carriage of respiratory pathogens are available. Oral administration of an LGG-containing probiotic mixture reduced nasal colonization of Gram-positive pathogens in adults  but did not affect nasopharyngeal carriage of S. pneumoniae in otitis-prone children . Furthermore, nasal delivery of Lactobacillus rhamnosus (strain LB2) did not affect the nasopharyngeal carriage of S. pneumoniae in children with secretory otitis media . LGG may provide a more systemic benefit, as lactobacilli have been shown to possess immunomodulatory and vaccine adjuvant properties [44, 45]. However, a probiotic known to colonize the respiratory tract, such as Streptococcus salivarius, may be more likely to prevent colonization of respiratory pathogens.
The principal finding from our study is that the probiotic LGG can reduce adherence of pneumococci to epithelial cells in an in vitro model. As LGG was effective in inhibiting adherence in the pre- and co- addition assays but less so in the post-addition assay, our data suggest that it would be more effective as a preventative strategy. Our findings support the notion that probiotics can be used as an additional strategy to prevent pneumococcal colonization and hence disease in early life. However, more research is needed to increase understanding of the mechanisms of probiotic action and identify what strategies (type of probiotic, mode, dose, and timing of administration) may be most effective in clinical settings.
Bacterial strains, cells and culture conditions
The pneumococcal isolates used in this study are described in Table 1. Bacterial isolates were obtained from our own culture collection or provided with permission from investigators from previous ethically-approved research. As no new sample collection or animal experiments were performed as part of this study, no additional ethics approval was required. Lactobacillus rhamnosu s strain GG (LGG) was obtained from Dicoflor capsules (Dicofarm, Italy). Pneumococci and LGG were cultured on horse blood agar (HBA; Oxoid, Australia) and de Man, Rogosa and Sharpe (MRS) agar (Oxoid, England) supplemented with 0.5% L-cysteine, respectively. For the adherence assay, pneumococci and LGG were cultured in Todd-Hewitt broth (Oxoid, England) supplemented with 0.5% yeast extract (Oxoid, England) or MRS broth (Oxoid, England), respectively. The human epithelial cell line CCL-23 was utilized (American Type Culture Collection, USA). Cells were maintained in modified Eagle’s medium (MEM) (Thermo Scientific, USA) supplemented with 10% fetal bovine serum (Thermo Scientific, Australia), 2 mM L-glutamine and 20 mM 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid. The concentration of fetal bovine serum was reduced to 5% for the adherence assays. Bacteria and epithelial cells were grown at 37°C and in 5% CO2 for maintenance and all assays.
Epithelial cells were seeded overnight at 1.5 x 105 cells /ml in a 24-well tray (Nunc, Denmark). The cells were then washed with prewarmed PBS and 500 μl MEM added. S. pneumoniae isolates were grown to mid-log phase, centrifuged at 1820 x g, and resuspended in 0.85% NaCl to a concentration of approximately 1 x 108 CFU/ml. A 10 μl inoculum containing 7.2 x 105 CFU (95%CI 5.8 x 105, 8.7 x 105 CFU) was added to the epithelial cells. PBS was used as a negative control and 100 U/ml heparin (Pfizer, Australia) was used as a positive control for blocking pneumococcal adherence. All assay conditions were performed in duplicate wells. The tray was centrifuged at 114 x g for five minutes and then incubated for three hours at 37°C, after which the medium was removed and the cells were gently washed three times with pre-warmed PBS to remove non cell-associated bacteria. Cells were lysed with 0.1% digitonin (Sigma-Aldrich, Australia) and viable counts of pneumococci were obtained by serial dilution and duplicate plating on HBA. Pneumococcal adherence was calculated as the percent of the original inoculum recovered at the end of the assay. LGG adherence was determined in a similar manner except that the samples were plated on MRS agar. To measure pneumococcal invasion, after the three hour incubation period, the culture medium was removed and the cells washed and incubated for another two hours with media containing 5 μg/ml penicillin and 100 μg/ml gentamicin to kill extracellular bacteria. The antibiotic-containing medium was then removed, after which the epithelial cells were lysed with digitonin and the number of CFU/ml determined. To determine the effect of LGG on pneumococcal adherence, LGG was added at 4.8 x 107 CFU (95%CI: 3.9 x 107, 5.8 x 107) or 4.8 x 106 CFU (95%CI: 3.9 x 106, 5.8 x 106 CFU) one hour before (pre-addition), at the same time as (co-addition) or one hour after (post-addition) pneumococci were added. Adherence is reported as percentage of adhering bacteria normalized to the ‘pneumococcal-only’ control (no heparin or LGG). Cell-free culture supernatants were prepared by collecting assay media after the 3 h incubation step, removing debris by centrifugation at 1820 x g for 3 min before passing resultant supernatants through a 0.22 μm pore size syringe filter (Millipore).
Detection of cytokines and chemokines by multiplex bead array
Concentrations of IL-1β, TNF-α, IL-6, and IL-8 were determined in epithelial cell culture supernatants following a three hour pneumococcal adherence assay using a multiplex method. Beadmates consisting of Beadlyte anti-cytokine beads and matched anti-cytokine biotinylated reporters were used according to the manufacturer’s protocol (Millipore, USA). In brief, 25 μl of bead preparation were incubated with 50 μl of standards, controls and samples in a 96 well plate overnight with shaking at 4°C. All culture supernatant samples were assayed undiluted in duplicate. The plate was washed twice and incubated for one hour at room temperature with 50 μl/well of detection antibodies, prior to a 30 minute incubation with 50 μl/well of streptavidin-phycoerythrin reagent. The plate was then washed twice and beads re-suspended in 100 μl/well sheath fluid before reading on a Luminex 200 Bio-analyzer (Luminex Corporation, USA). The lower limit of detection for all cytokines/chemokines was 0.13 pg/ml. Data analysis was performed using the LuminexIS 2.3 software (Luminex Corporation, USA).
Data were analysed using GraphPad Prism version 5.04 for Windows (GraphPad Software, USA). All data are presented as mean ± standard deviation (SD) unless otherwise specified. One-way analysis of variance (ANOVA) with Bonferroni’s post hoc test was used to analyze differences between groups for all data except comparisons of mid-log versus stationary growth in adherence and invasion levels, in which case the Mann–Whitney test was used. All experiments were performed on at least three separate occasions, except where otherwise indicated. P < 0.05 was considered significant for all analyses.
This work was supported by funding from the Murdoch Childrens Research Institute and the Victorian Government’s Operational Infrastructure Support Program. For the provision of isolates we thank Fiona Russell and the Fiji pneumococcal project team; Samir Saha and the staff of the Child Health Research Foundation, Dhaka Shishu Hospital, and the families who participated in the Pneumococcal carriage project; and Kate O’Brien, Johns Hopkins Bloomberg School of Public Health.
- O’Brien KL, Wolfson LJ, Watt JP, Henkle E, Deloria-Knoll M, McCall N, Lee E, Mulholland K, Levine OS, Cherian T: Burden of disease caused by Streptococcus pneumoniae in children younger than 5 years: global estimates. Lancet. 2009, 374 (9693): 893-902. 10.1016/S0140-6736(09)61204-6.PubMedView ArticleGoogle Scholar
- Gray BM, Converse GM, Dillon HC: Epidemiologic studies of Streptococcus pneumoniae in infants: acquisition, carriage, and infection during the first 24 months of life. J Infect Dis. 1980, 142 (6): 923-933. 10.1093/infdis/142.6.923.PubMedView ArticleGoogle Scholar
- Kwambana BA, Barer MR, Bottomley C, Adegbola RA, Antonio M: Early acquisition and high nasopharyngeal co-colonisation by Streptococcus pneumoniae and three respiratory pathogens amongst Gambian new-borns and infants. BMC Infect Dis. 2011, 11: 175-10.1186/1471-2334-11-175.PubMedPubMed CentralView ArticleGoogle Scholar
- Johnson HL, Deloria-Knoll M, Levine OS, Stoszek SK, Freimanis Hance L, Reithinger R, Muenz LR, O’Brien KL: Systematic evaluation of serotypes causing invasive pneumococcal disease among children under five: the pneumococcal global serotype project. PLoS Med. 2010, 7 (10): e1000348-10.1371/journal.pmed.1000348.PubMedPubMed CentralView ArticleGoogle Scholar
- Mulholland K, Satzke C: Serotype replacement after pneumococcal vaccination. Lancet. 2012, 379 (9824): 1387-PubMedView ArticleGoogle Scholar
- FAO/WHO 2001: Joint FAO/WHO Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria. 2001, Cordoba, Argentina, 1 to 4 OctoberGoogle Scholar
- Macfarlane GT, Cummings JH: Probiotics, infection and immunity. Curr Opin Infect Dis. 2002, 15 (5): 501-506. 10.1097/00001432-200210000-00008.PubMedView ArticleGoogle Scholar
- Sullivan A, Nord CE: Probiotics and gastrointestinal diseases. J Intern Med. 2005, 257 (1): 78-92. 10.1111/j.1365-2796.2004.01410.x.PubMedView ArticleGoogle Scholar
- Collado MC, Meriluoto J, Salminen S: Role of commercial probiotic strains against human pathogen adhesion to intestinal mucus. Lett Appl Microbiol. 2007, 45 (4): 454-460. 10.1111/j.1472-765X.2007.02212.x.PubMedView ArticleGoogle Scholar
- Jeon SG, Kayama H, Ueda Y, Takahashi T, Asahara T, Tsuji H, Tsuji NM, Kiyono H, Ma JS, Kusu T: Probiotic Bifidobacterium breve induces IL-10-producing Tr1 cells in the colon. PLoS Pathog. 2012, 8 (5): e1002714-10.1371/journal.ppat.1002714.PubMedPubMed CentralView ArticleGoogle Scholar
- Anderson RC, Cookson AL, McNabb WC, Kelly WJ, Roy NC: Lactobacillus plantarum DSM 2648 is a potential probiotic that enhances intestinal barrier function. FEMS Microbiol Lett. 2010, 309 (2): 184-192.PubMedGoogle Scholar
- Licciardi PV, Toh ZQ, Dunne E, Wong SS, Mulholland EK, Tang M, Robins-Browne RM, Satzke C: Protecting against pneumococcal disease: critical interactions between probiotics and the airway microbiome. PLoS Pathog. 2012, 8 (6): e1002652-10.1371/journal.ppat.1002652.PubMedPubMed CentralView ArticleGoogle Scholar
- Popova M, Molimard P, Courau S, Crociani J, Dufour C, Le Vacon F, Carton T: Beneficial effects of probiotics in upper respiratory tract infections and their mechanical actions to antagonize pathogens. J Appl Microbiol. 2012, 113 (6): 1305-1318. 10.1111/j.1365-2672.2012.05394.x.PubMedView ArticleGoogle Scholar
- Tanaka A, Seki M, Yamahira S, Noguchi H, Kosai K, Toba M, Morinaga Y, Miyazaki T, Izumikawa K, Kakeya H: Lactobacillus pentosus strain b240 suppresses pneumonia induced by Streptococcus pneumoniae in mice. Lett Appl Microbiol. 2011, 53 (1): 35-43. 10.1111/j.1472-765X.2011.03079.x.PubMedView ArticleGoogle Scholar
- Racedo S, Villena J, Medina M, Aguero G, Rodriguez V, Alvarez S: Lactobacillus casei administration reduces lung injuries in a Streptococcus pneumoniae infection in mice. Microbes Infect. 2006, 8 (9–10): 2359-2366.PubMedView ArticleGoogle Scholar
- Medina M, Villena J, Salva S, Vintini E, Langella P, Alvarez S: Nasal administration of Lactococcus lactis improves local and systemic immune responses against Streptococcus pneumoniae. Microbiol Immunol. 2008, 52 (8): 399-409. 10.1111/j.1348-0421.2008.00050.x.PubMedView ArticleGoogle Scholar
- Princivalli MS, Paoletti C, Magi G, Palmieri C, Ferrante L, Facinelli B: Lactobacillus rhamnosus GG inhibits invasion of cultured human respiratory cells by prtF1-positive macrolide-resistant group A streptococci. Lett Appl Microbiol. 2009, 48 (3): 368-372. 10.1111/j.1472-765X.2008.02540.x.PubMedView ArticleGoogle Scholar
- Gluck U, Gebbers JO: Ingested probiotics reduce nasal colonization with pathogenic bacteria (Staphylococcus aureus, Streptococcus pneumoniae, and beta-hemolytic streptococci). Am J Clin Nutr. 2003, 77 (2): 517-520.PubMedGoogle Scholar
- Tonnaer EL, Hafmans TG, Van Kuppevelt TH, Sanders EA, Verweij PE, Curfs JH: Involvement of glycosaminoglycans in the attachment of pneumococci to nasopharyngeal epithelial cells. Microbes Infect. 2006, 8 (2): 316-322. 10.1016/j.micinf.2005.06.028.PubMedView ArticleGoogle Scholar
- Ruiz V, Rodriguez-Cerrato V, Huelves L, Del Prado G, Naves P, Ponte C, Soriano F: Adherence of Streptococcus pneumoniae to polystyrene plates and epithelial cells and the antiadhesive potential of albumin and xylitol. Pediatr Res. 2011, 69 (1): 23-27. 10.1203/PDR.0b013e3181fed2b0.PubMedView ArticleGoogle Scholar
- de Leeuw E, Li X, Lu W: Binding characteristics of the Lactobacillus brevis ATCC 8287 surface layer to extracellular matrix proteins. FEMS Microbiol Lett. 2006, 260 (2): 210-215. 10.1111/j.1574-6968.2006.00313.x.PubMedView ArticleGoogle Scholar
- Lorca G, Torino MI, Font de Valdez G, Ljungh AA: Lactobacilli express cell surface proteins which mediate binding of immobilized collagen and fibronectin. FEMS Microbiol Lett. 2002, 206 (1): 31-37. 10.1111/j.1574-6968.2002.tb10982.x.PubMedView ArticleGoogle Scholar
- Yamaguchi M, Terao Y, Mori Y, Hamada S, Kawabata S: PfbA, a novel plasmin- and fibronectin-binding protein of Streptococcus pneumoniae, contributes to fibronectin-dependent adhesion and antiphagocytosis. J Biol Chem. 2008, 283 (52): 36272-36279. 10.1074/jbc.M807087200.PubMedPubMed CentralView ArticleGoogle Scholar
- Hermans PW, Adrian PV, Albert C, Estevao S, Hoogenboezem T, Luijendijk IH, Kamphausen T, Hammerschmidt S: The streptococcal lipoprotein rotamase A (SlrA) is a functional peptidyl-prolyl isomerase involved in pneumococcal colonization. J Biol Chem. 2006, 281 (2): 968-976.PubMedView ArticleGoogle Scholar
- Spinler JK, Taweechotipatr M, Rognerud CL, Ou CN, Tumwasorn S, Versalovic J: Human-derived probiotic Lactobacillus reuteri demonstrate antimicrobial activities targeting diverse enteric bacterial pathogens. Anaerobe. 2008, 14 (3): 166-171. 10.1016/j.anaerobe.2008.02.001.PubMedPubMed CentralView ArticleGoogle Scholar
- Wescombe PA, Heng NC, Burton JP, Chilcott CN, Tagg JR: Streptococcal bacteriocins and the case for Streptococcus salivarius as model oral probiotics. Future Microbiol. 2009, 4 (7): 819-835. 10.2217/fmb.09.61.PubMedView ArticleGoogle Scholar
- Bergeron Y, Ouellet N, Deslauriers AM, Simard M, Olivier M, Bergeron MG: Cytokine kinetics and other host factors in response to pneumococcal pulmonary infection in mice. Infect Immun. 1998, 66 (3): 912-922.PubMedPubMed CentralGoogle Scholar
- Xu F, Droemann D, Rupp J, Shen H, Wu X, Goldmann T, Hippenstiel S, Zabel P, Dalhoff K: Modulation of the inflammatory response to Streptococcus pneumoniae in a model of acute lung tissue infection. Am J Respr Cell Mol Biol. 2008, 39 (5): 522-529. 10.1165/rcmb.2007-0328OC.View ArticleGoogle Scholar
- Heikkinen T, Ghaffar F, Okorodudu AO, Chonmaitree T: Serum interleukin-6 in bacterial and nonbacterial acute otitis media. Pediatr. 1998, 102 (2 Pt 1): 296-299.View ArticleGoogle Scholar
- Schmeck B, Moog K, Zahlten J, van Laak V, N’Guessan PD, Opitz B, Rosseau S, Suttorp N, Hippenstiel S: Streptococcus pneumoniae induced c-Jun-N-terminal kinase- and AP-1 -dependent IL-8 release by lung epithelial BEAS-2B cells. Respir Res. 2006, 7: 98-10.1186/1465-9921-7-98.PubMedPubMed CentralView ArticleGoogle Scholar
- Zhang L, Xu YQ, Liu HY, Lai T, Ma JL, Wang JF, Zhu YH: Evaluation of Lactobacillus rhamnosus GG using an Escherichia coli K88 model of piglet diarrhoea: Effects on diarrhoea incidence, faecal microflora and immune responses. Vet Microbiol. 2010, 141 (1–2): 142-148.PubMedView ArticleGoogle Scholar
- Liu F, Li G, Wen K, Bui T, Cao D, Zhang Y, Yuan L: Porcine small intestinal epithelial cell line (IPEC-J2) of rotavirus infection as a new model for the study of innate immune responses to rotaviruses and probiotics. Viral Immunol. 2010, 23 (2): 135-149. 10.1089/vim.2009.0088.PubMedPubMed CentralView ArticleGoogle Scholar
- Nandakumar NS, Pugazhendhi S, Madhu Mohan K, Jayakanthan K, Ramakrishna BS: Effect of Vibrio cholerae on chemokine gene expression in HT29 cells and its modulation by Lactobacillus GG. Scand J Immunol. 2009, 69 (3): 181-187. 10.1111/j.1365-3083.2008.02214.x.PubMedView ArticleGoogle Scholar
- Lopez M, Li N, Kataria J, Russell M, Neu J: Live and ultraviolet-inactivated Lactobacillus rhamnosus GG decrease flagellin-induced interleukin-8 production in Caco-2 cells. J Nutr. 2008, 138 (11): 2264-2268. 10.3945/jn.108.093658.PubMedView ArticleGoogle Scholar
- Marriott HM, Gascoyne KA, Gowda R, Geary I, Nicklin MJ, Iannelli F, Pozzi G, Mitchell TJ, Whyte MK, Sabroe I: Interleukin-1beta regulates CXCL8 release and influences disease outcome in response to Streptococcus pneumoniae, defining intercellular cooperation between pulmonary epithelial cells and macrophages. Infect Immun. 2012, 80 (3): 1140-1149. 10.1128/IAI.05697-11.PubMedPubMed CentralView ArticleGoogle Scholar
- Lortat-Jacob H, Grosdidier A, Imberty A: Structural diversity of heparan sulfate binding domains in chemokines. Proc Natl Acad Sci USA. 2002, 99 (3): 1229-1234. 10.1073/pnas.032497699.PubMedPubMed CentralView ArticleGoogle Scholar
- Webb LM, Ehrengruber MU, Clark-Lewis I, Baggiolini M, Rot A: Binding to heparan sulfate or heparin enhances neutrophil responses to interleukin 8. Proc Natl Acad Sci USA. 1993, 90 (15): 7158-7162. 10.1073/pnas.90.15.7158.PubMedPubMed CentralView ArticleGoogle Scholar
- Rautava S, Salminen S, Isolauri E: Specific probiotics in reducing the risk of acute infections in infancy–a randomised, double-blind, placebo-controlled study. Br J Nutr. 2009, 101 (11): 1722-1726. 10.1017/S0007114508116282.PubMedView ArticleGoogle Scholar
- Kukkonen K, Savilahti E, Haahtela T, Juntunen-Backman K, Korpela R, Poussa T, Tuure T, Kuitunen M: Long-term safety and impact on infection rates of postnatal probiotic and prebiotic (synbiotic) treatment: randomized, double-blind, placebo-controlled trial. Pediatr. 2008, 122 (1): 8-12. 10.1542/peds.2007-1192.View ArticleGoogle Scholar
- Hojsak I, Abdovic S, Szajewska H, Milosevic M, Krznaric Z, Kolacek S: Lactobacillus GG in the prevention of nosocomial gastrointestinal and respiratory tract infections. Pediatr. 2010, 125 (5): e1171-1177. 10.1542/peds.2009-2568.View ArticleGoogle Scholar
- Hojsak I, Snovak N, Abdovic S, Szajewska H, Misak Z, Kolacek S: Lactobacillus GG in the prevention of gastrointestinal and respiratory tract infections in children who attend day care centers: a randomized, double-blind, placebo-controlled trial. Clin Nutr. 2010, 29 (3): 312-316. 10.1016/j.clnu.2009.09.008.PubMedView ArticleGoogle Scholar
- Hatakka K, Blomgren K, Pohjavuori S, Kaijalainen T, Poussa T, Leinonen M, Korpela R, Pitkaranta A: Treatment of acute otitis media with probiotics in otitis-prone children-a double-blind, placebo-controlled randomised study. Clin Nutr. 2007, 26 (3): 314-321. 10.1016/j.clnu.2007.01.003.PubMedView ArticleGoogle Scholar
- Skovbjerg S, Roos K, Holm SE, Grahn Hakansson E, Nowrouzian F, Ivarsson M, Adlerberth I, Wold AE: Spray bacteriotherapy decreases middle ear fluid in children with secretory otitis media. Arch Dis Child. 2009, 94 (2): 92-98. 10.1136/adc.2008.141002.PubMedView ArticleGoogle Scholar
- Wells JM: Immunomodulatory mechanisms of lactobacilli. Microb Cell Fact. 2011, 10 (Suppl 1): S17-10.1186/1475-2859-10-S1-S17.PubMedPubMed CentralView ArticleGoogle Scholar
- Isolauri E, Joensuu J, Suomalainen H, Luomala M, Vesikari T: Improved immunogenicity of oral D x RRV reassortant rotavirus vaccine by Lactobacillus casei GG. Vaccine. 1995, 13 (3): 310-312. 10.1016/0264-410X(95)93319-5.PubMedView ArticleGoogle Scholar
- Walls T, Power D, Tagg J: Bacteriocin-like inhibitory substance (BLIS) production by the normal flora of the nasopharynx: potential to protect against otitis media?. J Med Microbiol. 2003, 52 (Pt 9): 829-833.PubMedView ArticleGoogle Scholar
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