Skip to main content
  • Research article
  • Open access
  • Published:

Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe



Replication and transcription, the two key functions of DNA, require unwinding of the DNA double helix. It has been shown that replication origins in the budding yeast, Saccharomyces cerevisiae contain an easily unwound stretch of DNA. We have used a recently developed method for determining the locations and degrees of stress-induced duplex destabilization (SIDD) for all the reported replication origins in the genome of the fission yeast, Schizosaccharomyces pombe.


We have found that the origins are more susceptible to SIDD as compared to the non-origin intergenic regions (NOIRs) and genes. SIDD analysis of many known origins in other eukaryotes suggests that SIDD is a common property of replication origins. Interestingly, the previously shown deletion-dependent changes in the activities of the origins of the ura4 origin region on chromosome 3 are paralleled by changes in SIDD properties, suggesting SIDD’s role in origin activity. SIDD profiling following in silico deletions of some origins suggests that many of the closely spaced S. pombe origins could be clusters of two or three weak origins, similar to the ura4 origin region.


SIDD appears to be a highly conserved, functionally important property of replication origins in S. pombe and other organisms. The distinctly low SIDD scores of origins and the long range effects of genetic alterations on SIDD properties provide a unique predictive potential to the SIDD analysis. This could be used in exploring different aspects of structural and functional organization of origins including interactions between closely spaced origins.


The duplication of genomic DNA in eukaryotic cells is accomplished by replication forks emanating bidirectionally from a large number of replication origins distributed throughout the genome [1, 2]. Replication origins of Saccharomyces cerevisiae, the best characterized eukaryotic origins, are confined to specific DNA regions of 100–200 bp known as autonomously replicating sequences or ARS elements as the plasmids containing them can replicate autonomously in yeast cells [3, 4]. The S. cerevisiae ARS elements are marked by the presence of two essential features, a close match to an 11 to15-bp ARS consensus sequence (ACS) and a stretch of easily unwound DNA next to ACS at the 3 end of its T-rich strand [57]. The ACS is occupied by the origin recognition complex (ORC) and other initiator proteins [8] and the adjacent easily unwound region facilitates double helix opening for initiation of replication. Mutations in ACS that destroy ORC binding or in the easily unwound region that increase its stability also cause loss of origin activity [7]. Although the ACS is essential for origin activity, it is not sufficient and only a small fraction of all genomic ACSs (~500 out of ~12000 ACS matches) is associated with active origins in S. cerevisiae. However, its presence has been helpful in precisely locating origins in broad origin regions mapped in genome-wide studies [915]. Unlike S. cerevisiae, the replication origins of all other studied eukaryotic cells lack a conserved nucleotide sequence.

Replication origins have also been extensively studied in the fission yeast, Schizosaccharomyces pombe, where they correspond, mostly but not always [16], to ARS elements. S. pombe ARS elements are ~1 kb in size and they lack any known conserved nucleotide sequences [17] like other eukaryotic origins. In addition to 54 precisely localized origins by the two-dimensional agarose gel electrophoresis origin mapping technique (2D technique) and DNA combing [1826], different genome-wide origin mapping studies have located several hundred to nearly one thousand potential origins in S. pombe[11, 25, 2729]. All these origins are confined to the intergenic regions (IRs), which usually have higher AT content than the genomic average. Most of them are inefficient, firing only in a small fraction of a cell population, and the closely spaced origins seem to interact with each other in a hierarchical fashion [18]. Replication origins of S. pombe have not been extensively analyzed for their helical stability and the destabilization properties of the origin-containing intergenic regions (OIRs) remain to be known.

Of the three methods developed to analyze duplex destabilization of any given stretch of DNA, those used by MELTMAP [30] and WEBTHERMODYN [31] are based only on the local nucleotide composition while the one used by WEBSIDD [32] also takes into account the effects of superhelical stress occurring in vivo on strand opening behaviors of all the base pairs in a topologically constrained domain. The global coupling of strand opening behaviors results into widespread changes in destabilization properties of all the base-pairs in the domain and a deletion/mutation in one region may cause such changes in regions several kilo-base away from it [33]. The computation of the destabilization energy, G(x), also called SIDD (stress-induced duplex destabilization) energy, using the WEBSIDD tool has been shown to predict accurately the location and extent of destabilization of different regulatory regions, promoters and replication origins, in viral, bacterial and yeast genomes and in some cases it has been found to be important for the origin activity [13, 3335]. The SIDD analysis results for S. cerevisiae origins have been found not only consistent with that of the duplex unwinding element (DUE) analysis by WEBTHERMODYN but also more informative in several ways because of its above-mentioned features [7, 33].

In this study we have analyzed the SIDD profile of all known replication origins of S. pombe mapped previously using different techniques. Our results show that S. pombe origins are more susceptible to stress-induced duplex destabilization than their adjacent genes and non-origin intergenes and that, in case of closely spaced origins, the extent of destabilization appears to influence the origin activity.


Sequence data collection

A fixed window size of 5 kb, unless otherwise mentioned, was used for all origins which have been mapped earlier to relatively smaller regions using the 2D technique, bioinformatics [25] or the microarray methods [27, 28] with the origins placed at the center. Origins larger than 5 kb were analyzed within the coordinates mentioned in the referred papers. Once boundaries were marked, sequences were downloaded from the S. pombe GeneDB, modified version of March-04-2011. The randomly selected comparison regions, genes and intergenes, were analyzed similarly.

Calculation of SIDD profiles

We used WEBSIDD server [32] to determine SIDD profile of previously reported origins, genes and non-origin intergenes using fixed window sizes as mentioned above. The conditions for the assessment of superhelical denaturation and the basis of computation have been described (WEBSIDD manual).


The SIDD profiling using the WEBSIDD results in a plot between the destabilization energy, G(x), under specified growth conditions and degree of superhelicity, and base-pair position. Under these conditions, the base-pairs with a G(x) value close to 10 kcal/mol are as stable as they would be in a relaxed molecule and those with a G(x) value near 0 kcal/mol are highly destabilized. The G(x) values between 0–10 kcal/mol show positions of intermediate stability [33]. Even a partial destabilization of a DNA region caused by a few kcal/mol lowering of G(x) will significantly facilitate its opening by other factors like helicases. SIDD analysis of S. cerevisiae replication origins has shown earlier that they are highly susceptible to superhelically driven DNA duplex destabilization [33]. To find out whether the replication origins of S. pombe are similarly destabilized, we performed SIDD calculations of all major reported replication origins in this organism. Because the boundaries of the topologically constrained domains occurring in vivo are not known and because of the variability of SIDD profile from window to window and for different sized windows centered on the same origin (Figure 1), we thought it necessary to figure out the analysis parameters that would predict the origin locations most reliably. For this, we determined SIDD profiles of 48 replication origins detected earlier using a two-dimensional origin mapping technique [36] and DNA combing [22]. These gold standard techniques are capable of physically mapping replication origins to short stretches of DNA ranging from several hundred base-pairs to a few kilo-bases. Initially, we used sliding windows which produced extremely variable SIDD profiles and any given region appeared highly stable or unstable in different overlapping windows. This is probably due to the fact that in SIDD calculations the melting properties of different basepairs in a domain are influenced by each other, and, in a sliding window, the relative position of every basepair is altered. However when different sized windows were centered on the same region, the variations were greatly reduced, mostly confined to limited changes in the depths of the valleys and, sometimes, to their slightly shifted locations (Figure 1D-F). Table S1 shows the results obtained using one variable window, the OIR plus 1-kb flanking regions on both sides, and two fixed windows of 5 kb and10 kb, all centered on origins to determine SIDD profiles of all 48 2D origins. Although all the origins appeared to contain SIDD valleys in all 3 windows, the SIDD valleys generally appeared shallower with increasing window size (e.g. Figure 1E). The average lowest G(x) for the 48 origins in the variable window, 5-kb window and 10-kb window analyses was 1.58-, 1.65- and 2.56- kcal/mol, respectively. Within the 5-kb windows for these 48 2D-gel-verified origins, valleys bottoms had G(x) values between 1.23 and 2.48 kcal/mol (Additional file 1: Table S1). All further analysis was done using a fixed window of 5 kb unless otherwise mentioned.

Figure 1
figure 1

Variability patterns of SIDD profiles in sliding and fixed windows. (A-C) Variability of SIDD profiles of Ori1098 in sliding windows. Note that the deep SIDD valley corresponding to the origin (grey bar) in the center of a 5-kb window (A) becomes shallow on sliding 1 kb from left to right (B) and shallower on sliding 1kb from right to left (C). (D-F) The locations of the SIDD valleys show little or no alteration although their depths change to different extents between 5-kb and 10-kb windows centered on an origin.

Next, we performed SIDD analysis of 355 AT islands [25], 460 origins mapped by high-resolution microarray ChIP-chip analysis of the locations of ORC and MCM subunits [28] and 476 weak origins mapped by copy number method [37]. Most of these origins are confined to relatively short stretches of DNA. Because the SIDD properties of AT islands and ChIP-chip origins were indistinguishable from each other and because most of them overlap with each other, we merged them together as 564 ‘origins’ for presentation (Additional file 2: Table S2). The 440 copy number origins which did not match with the origins are listed as weak origins. All the origins and weak origins showed the presence of one, two or sometimes three or more significantly destabilized IRs (Figure 2). All but four of the 564 origins (99.3%) were found to be highly unstable with the average minimum G(x) value 1.85 kcal/mol (Table 1, Additional file 2: Table S2). The four OIRs showing a minimum G(x) greater than 9.0 kcal/mol associated with Ori1003, Ori2024, Ori2030 and AT-1120, are all flanked by destabilized regions on one or both sides (see the profile of Ori1003 in Figure 2G). Among the ChIP-chip origins, the average lowest G(x) for the early origins (1.77 kcal/mol) differed only slightly from that of the late origins (1.88 kcal/mol). The 440 weak origins showed an average lowest G(x) value 2.46 kcal/mol. Similarly analyzed 360 genes appeared to be highly stable with an average lowest G(x) value 8.47 kcal/mol. NOIRs showed an intermediate level of stability with an average lowest G(x) value 3.16 kcal/mol for 434 intergenes. Using the 5-kb window analysis, 91% origins, 67% weak origins, 43% NOIRs and <2% genes coincided with the lowest SIDD valley in their window. The number of origins, genes and NOIRs studied, the averages of their lowest G(x) values and their AT contents are given in Table 1.

Figure 2
figure 2

Representative patterns of the SIDD profiles of different genomic entities. (A-G) origins, (H) a NOIR and, (I) a gene. All these origins except (D) and (G) are 2D-gel proven. The locations of the origins/AT islands are marked with grey bars. Note in (G) the presence of a deep SIDD valley not overlapping but close to Ori1003, one of the four origins found to be highly stable in this study.

Table 1 Averages of the lowest SIDD values, sizes and AT contents of different entities

The distribution patterns of the lowest G(x) values of these entities are clearly distinguishable from each other (Figure 3A). The origins comprised a vast majority (>76% to >80%) of all the intergenes falling in any of the three arbitrarily fixed lowest G(x) cut off values of 2.0, 2.5 and, 3.0 kcal/mol (Table 2). Since all the genomic origin mapping studies, whose origin mapping data have been used for the present study, have failed to report at least 3 2-D proven origins, ori2, ori4 and ori5, in a 75-kb region [19], this ratio is likely to increase in favor of origins as future studies come up with additional origins.

Figure 3
figure 3

Origins are more susceptible to SIDD than NOIRs and genes. (A) Distribution patterns of the lowest G(x) values of 564 origins (ChIP-chip origins and AT-islands) [25, 28] (black dots), 440 weak origins [37] (grey dots), 434 NOIRs (magenta dots) and 360 genes (cyan dots). (B) The graph of AT% vs lowest G(x) values of the four analyzed entities, ChIP-chip origins and ATislands (black dots), weak origins (grey dots), NOIRs (magenta dots) and genes (cyan dots) in the 5-kb context.

Table 2 Majority of origins are more susceptible to SIDD than NOIRs and genes

We used Wilcoxon-Mann–Whitney rank sum test to determine the statistical significance of the observed differences between the lowest G(x) values of OIRs and NOIRs and found that they differed significantly (P=<0.05) for both, the origins and the weak origins. The origins and the weak origins also differed significantly from each other (P=<0.05) in their G(x) values. We conclude that the susceptibility to stress-induced destabilization of different entities of the S. pombe genome shows the following pattern: origins>weak origins>NOIRs>genes.

To find out the functional significance of this coincidence, we performed SIDD analysis of the ura4 origin region [18], one of the best genetically dissected chromosomal origin regions in S. pombe. This origin region contains two strong ARS elements, ars3002, ars3003 and one weak ARS element, ars3004, which later turned out to be a part of extended ars3002[39], within a ~5-kb region on chromosome 3. Each of the two strong ARS elements functions as a replication origin in the chromosome. When any one of them is deleted the origin activity seems to be transferred to the other. When both of them are deleted, only then the weak ARS, ars3004, becomes active as origin [18]. The SIDD profile of the region showed that both the chromosomally active origins, ars3002 and ars3003, were stress-destabilized in the wild type cells (Figure 4A) and deletion of either of them resulted into the disappearance of the corresponding SIDD valleys and formation of deeper valleys under the remaining ARS (Figure 4B and C). Deletion of both of them resulted into destabilization of ars3004 which had been shown to get activated as an origin in this strain (Figure 4D). These results strongly suggested that the extent of SIDD could be an important factor influencing the origin activity.

Figure 4
figure 4

Alterations in the origin activity are paralleled by SIDD changes in the ura4 origin region. Relationship between the extent of SIDD and origin activity in the ura4 origin region in (A) wild-type, and, (B-D) ARS deletion S. pombe strains [18]. The map of the concerned region, locations of ARS elements, restriction sites (Av=AvrII, B=BamHI, Bg=BglII, Bs=BstBI, C=ClaI RI=EcoRI, RV=EcoRV, H=HinDIII, N=NruI, Ns=NsiI,S=SmaI) and the 2D autoradiograms of different fragments shown on the top of each section have been reproduced from [18] with permission. The arrows mark the bubble arc signal, indicative of the origin activity of the detected fragment. The lowest G(x) value for each ARS element is shown in kcal/mol near the valley.

As mentioned above, many origin containing regions showed the presence of more than one destabilized intergenes. Are these origins similar to the ura4 origin region in terms of interactive SIDD properties? To test this possibility, we generated in silico deletions of the closely spaced stress-destabilized intergenes in thirty-five randomly chosen such origins in different combinations and performed SIDD analysis of these regions. Indeed, we found a transition of SIDD valleys from one intergene to another akin to the ura4 deletion strains in all of them. The SIDD profiles of two such origins and their deleted versions are shown in Figure 5.

Figure 5
figure 5

Other closely spaced stress-destabilized intergenes show SIDD interactions akin to the ura4 origin region. SIDD profiles of two ChIP-chip origins, Ori1092 (rip1) and Ori1047 (ars1), to check the interactions between the closely spaced stress destabilized intergenes following in silico deletions. In case of Ori1092, the intergenes were deleted in silico and replaced with ura4 gene somewhat mimicking the experiments done in [18]. The locations of the origins (dark grey bar), other SIDD valleys (light grey bar) and genes (top) are shown (S. pombe GeneDB). The lowest G(x) values are mentioned below the valleys.


We have analyzed SIDD profiles of different genomic regions containing three different but overlapping sets of replication origins mapped earlier by different groups, genes and non-origin intergenes of S. pombe. This is the first extensive SIDD profiling and comparative analysis of different genomic entities in S. pombe. A vast majority of origins colocalized with the IRs showing the lowest SIDD value. In fact, 99.3% of the 564 origins defined by ChIP-chip or AT content had lowest SIDD values (G(x) values) less than 6.07, and the four putative origins with SIDD values greater than 9 have not been verified as functional origins. It is known that the destabilization energy or the SIDD energy, G(x), is directly related to stability [33]. Therefore, S. pombe replication origins correspond to genomic regions susceptible to destabilization under stress. In fact, under the conditions of our analysis, there appears to be a gradient of stability among different genomic entities in which the origins are least stable followed by weak origins, NOIRs and the genes which are most stable.

Although the origins are more susceptible to stress-destabilization as compared to genes and non-origin intergenes in S. pombe, the depth of the SIDD valley alone may not be used to predict the genome-wide locations of origins because similar valleys are also present elsewhere in the genome. However, consideration of the AT-richness, a known attribute of S. pombe origins, makes them stand as a distinct class with the highest AT content and lowest free energy requirement for strand separation under stress. Plotting AT content versus lowest SIDD values (Figure 3B) shows a gradual increase in AT content and decrease in SIDD energy as we move from genes to NOIRs to OIRs. The lowest SIDD values and the AT contents of early- and late-firing origins [28] were found to be very similar (data not shown) suggesting no link between SIDD and the time of origin activation. Since clustered A/T stretches are essential components of fission yeast replication origins and AT-hooks of spOrc4 bind to asymmetrical As and Ts in origin regions [4042], it appears that the binding efficiency of ORC and the extent of stress-induced destabilization both cooperatively determine origin usage and compromising with any one or both of them would adversely affect the firing frequency of an origin.

Recently, a genome-wide comparative analysis of replication origins in three species of fission yeasts, S. pombe, S. octosporus and S. japonicus has revealed that in contrast to AT-rich origins of S. pombe and S. octosporus, origins of S. japonicus are marked by the presence of GC-rich regions [43]. Preliminary results from SIDD analysis of some defined S. japonicus ARS elements suggest the presence of SIDD-prone DNA in them (data not shown) suggesting that even the GC-rich origins of this organism are partially stress-destabilized.

The presence of an easily unwound region at the 3 end of the essential ARS consensus sequence, the binding site for the ORC proteins, is an essential feature of S. cerevisiae replication origins [7] and majority of them are stress-destabilized [33]. Because of a large origin size and the absence of any ACS like landmark, it would not be possible to deduce a similar relationship in case of S. pombe origins. However, notwithstanding the structural differences between the S. cerevisiae and S. pombe origins, SIDD appears to be a property common to both of them. This is anticipated as the presence of SIDD sites would facilitate conversion of double stranded DNA to single stranded form and ORC-assisted loading of other initiation factors on to origins. We found that the 217 ORC binding sites [28] associated with nucleosome free regions [44] are located in the same intergene, mostly within a few hundred basepairs (≤500 bp) of the SIDD sites.

It is very interesting that the earlier reported changes in the activities of the origins associated with the ura4 origin region following their systematic deletion are paralleled by SIDD changes. We previously interpreted these results by suggesting that closely spaced origins can interfere with each other. Whichever one fires first is likely to generate replication forks that will replicate the other origins in the cluster before those other origins have a chance to fire on their own. Consequently, deleting any single origin from a cluster would be predicted to increase the firing rate of the remaining origins. Our new analyses suggest an alternative mechanism: deleting one origin in a cluster may facilitate the firing of the other origins by contributing to the SIDD of each of the other origins. Perhaps both mechanisms contribute to the observed results. Future experiments may be able to discriminate between these possibilities by comparing the effects on the functions of the origins in clusters of deletions having large SIDD effects versus deletions having small SIDD effects.

Based on the interactive behavior of the SIDD properties of the closely spaced intergenes, we predict that more than half of all the origins in S. pombe genome are like the ura4 origin region having more than one closely spaced inefficient origins functioning synergistically. In vivo experiments would be required to find out if the observed deletion-dependent transitions of SIDD points are also followed by changes in firing frequency of the associated origins. The wide-spread alterations in SIDD properties following mutation in a DNA region [33, this study] could be utilized to study interactions between closely spaced origins (clusters of origins) in chromosomes of S. pombe, and probably other organisms also, to ascertain the functional organization of their replication origins.

Finally, SIDD analysis of the known origins in some other species like Kluvyeromyces lactis[45], Arabidposis thaliana[46], Candida albicans[47] and in humans [4850] (see Table 3) indicates that origins are stress destabilized in these organisms also. Despite tremendous variability in their nucleotide compositions, the facilitation of duplex opening encrypted in the regulatory regions, especially origins of different genomes as shown in this and many other earlier studies [7, 13, 3335], seems to be a widely conserved property. The results of this study extend similar earlier findings in S. cerevisiae and strongly support the idea that these properties in conjunction with other known origin attributes may be useful in predicting the locations of origins in other genomes too.

Table 3 The replication origins of other organisms are also stress-destabilized


SIDD analysis of replication origins and comparison regions of S. pombe shows that the origins are located in intergenic regions (OIRs) which are significantly more susceptible to strand separation under superhelical stress than NOIRs and genes. SIDD appears to be a widely conserved origin property that can be used to predict origin locations in conjunction with other known origin attributes, e.g., AT richness in case of S. pombe. The interactive nature of SIDD can also be used to predict interaction between closely-spaced origins as the deletion-induced changes in origin activity are accompanied by similar changes in degree of susceptibility to destabilization.

Availability of supporting data

The data sets supporting the results of this article are included within the article and its additional files.



Two-dimensional electrophoresis origin mapping technique


Autonomously replicating sequence


ARS core consensus sequence


Non-origin intergenic regions


Mini chromosome maintenance proteins


Origin recognition complex


Origin containing intergenic regions


Stress-induced duplex destabilization.


  1. Huberman JA, Riggs AD: On the mechanism of DNA replication in mammalian chromosomes. J Mol Biol. 1968, 32: 327-341. 10.1016/0022-2836(68)90013-2.

    Article  PubMed  CAS  Google Scholar 

  2. Hand R: Eukaryotic DNA: Organization of the genome for replication. Cell. 1978, 15: 317-325. 10.1016/0092-8674(78)90001-6.

    Article  PubMed  CAS  Google Scholar 

  3. Hsiao CL, Carbon J: High frequency transformation of yeast by plasmid containing the cloned yeast ARG gene. Proc Natl Acad Sci USA. 1979, 76: 3829-3833. 10.1073/pnas.76.8.3829.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  4. Stinchcomb DT, Struhl K, Davis RW: Isolation and characterization of yeast chromosomal replicator. Nature. 1979, 282: 39-43. 10.1038/282039a0.

    Article  PubMed  CAS  Google Scholar 

  5. Marahrens Y, Stillman B: A yeast chromosomal origin of DNA replication defined by multiple functional elements. Science. 1992, 255: 817-823. 10.1126/science.1536007.

    Article  PubMed  CAS  Google Scholar 

  6. Newlon CS, Theis JF: The structure and function of yeast ARS elements. Curr Opin Genet Dev. 1993, 3: 752-758. 10.1016/S0959-437X(05)80094-2.

    Article  PubMed  CAS  Google Scholar 

  7. Natale DA, Umek RM, Kowalski D: Ease of DNA unwinding is a conserved property of yeast replication origins. Nucleic Acids Res. 1993, 21: 555-560. 10.1093/nar/21.3.555.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  8. Bell SP, Dutta A: DNA replication in eukaryotic cells. Annual Review Biochem. 2002, 71: 333-374. 10.1146/annurev.biochem.71.110601.135425.

    Article  CAS  Google Scholar 

  9. Raghuraman MK, Winzeler EA, Collingwood D, Hunt S, Wodicka L, Conway A, Lockhart DJ, Davis RW, Brewer BJ, Fangman WL: Replication dynamics of the yeast genome. Science. 2001, 294: 115-121. 10.1126/science.294.5540.115.

    Article  PubMed  CAS  Google Scholar 

  10. Wyrick JJ, Aparicio JG, Chen T, Barnett JD, Jennings EG, Young RA, Bell SP, Aparicio OM: Genome-wide distribution of ORC and MCM proteins in Saccharomyces cerevisiae: high-resolution mapping of replication origins. Science. 2001, 294: 2357-2360. 10.1126/science.1066101.

    Article  PubMed  CAS  Google Scholar 

  11. Feng W, Collingwood D, Boeck ME, Fox LA, Alvino GM, Fangman WL, Raghuraman MK, Brewer BJ: Genomic mapping of single-stranded DNA in hydroxyurea challenged yeasts identifies origins of replication. Nat Cell Biol. 2006, 8: 148-155. 10.1038/ncb1358.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  12. Nieduszynski CA, Knox Y, Donaldson AD: Genome-wide identification of replication origins in yeast by comparative genomics. Genes Dev. 2006, 20: 1874-1879. 10.1101/gad.385306.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  13. Nieduszynski CA, Hiraga S, Prashanth A, Benham CJ, Donaldson AD: OriDB: a DNA replication origin database. Nucleic Acids Res. 2007, 35: D40-46. 10.1093/nar/gkl758.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  14. Berbenetz NM, Nislow C, Brown GW: Diversity of eukaryotic DNA replication origins revealed by genome-wide analysis of chromatin structure. PLoS Genet. 2010, 6: e1001092-10.1371/journal.pgen.1001092.

    Article  PubMed  PubMed Central  Google Scholar 

  15. Eaton ML, Galani K, Kang S, Bell SP, MacAlpine DM: Conserved nucleosome positioning defines replication origins. Genes Dev. 2010, 24: 748-753. 10.1101/gad.1913210.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  16. Dai J, Chuang RY, Kelly TJ: DNA replication origins in the Schizosaccharomyces pombe genome. Proc Natl Acad Sci U S A. 2005, 102: 337-342. 10.1073/pnas.0408811102.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  17. Masukata H, Huberman JA, Frattini MG, Kelly TJ: DNA replication. Schizosaccharomyces pombe: genetics genomics and beyond. Edited by: Egel R. 2004, Berlin, Heidelberg: Springer-Verlag, 73-99.

    Chapter  Google Scholar 

  18. Dubey DD, Zhu J, Carlson DL, Sharma K, Huberman JA: Three ARS elements contribute to the ura4 replication origin region in the fission yeast. Schizosaccharomyces pombe. EMBO J. 1994, 13: 3638-3647.

    PubMed  CAS  PubMed Central  Google Scholar 

  19. Dubey DD, Srivastava VK, Pratihar AS, Yadava MP: High density of weak replication origins in a 75-kb region of chromosome 2 of fission yeast. Genes Cells. 2010, 15: 1-12. 10.1111/j.1365-2443.2009.01363.x.

    Article  PubMed  CAS  Google Scholar 

  20. Gomez M, Antequera F: Organization of the DNA replication origins in the fission yeast genome. EMBO J. 1999, 18: 5683-5690. 10.1093/emboj/18.20.5683.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  21. Okuno Y, Okazaki T, Masukata H: Identification of a predominant replication origin in fission yeast. Nucleic Acids Res. 1997, 25: 530-536. 10.1093/nar/25.3.530.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  22. Patel PK, Arcangioli B, Baker SP, Bensimon A, Rhind N: DNA replication origins fire stochastically in fission yeast. Mol Biol Cell. 2006, 17: 308-316.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  23. Sanchez JA, Kim S-M, Huberman JA: Ribosomal DNA replication in the fission yeast. Schizosaccharomyces pombe. Exp Cell Res. 1998, 238: 220-230. 10.1006/excr.1997.3835.

    Article  PubMed  CAS  Google Scholar 

  24. Segurado M, Gomez M, Antequera F: Increased recombination intermediates and homologous integration hot spots at DNA replication origins. Mol Cell. 2002, 10: 907-916. 10.1016/S1097-2765(02)00684-6.

    Article  PubMed  CAS  Google Scholar 

  25. Segurado M, de Luis A, Antequera F: Genome-wide distribution of DNA replication origins at A+ T-rich islands in Schizosaccharomyces pombe. EMBO Rep. 2003, 4: 1048-1053. 10.1038/sj.embor.7400008.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  26. de Castro E, Soriano I, Martin L, Serrano R, Quintales L, Antequera F: Nucleosomal organization of replication origins and meiotic recombination hotspots in fission yeast. EMBO J. 2012, 31: 124-137.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  27. Heichinger C, Penkett CJ, Bahler J, Nurse P: Genome-wide characterization of fission yeast DNA replication origins. EMBO J. 2006, 25: 5171-5179. 10.1038/sj.emboj.7601390.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  28. Hayashi M, Katou Y, Itoh T, Tazumi M, Yamada Y, Takahashi T, Nakagawa T, Shirahige K, Masukata H: Genome-wide localization of Pre-RC sites and identification of replication origins in fission yeast. EMBO J. 2007, 26: 1327-1339. 10.1038/sj.emboj.7601585.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  29. Mickle KL, Ramanathan S, Rosebrock A, Oliva A, Chaudary A, Yompakdee C, Scott D, Leatherwood J, Huberman JA: Checkpoint independence of most replication origins in fission yeast. BMC Mol Biol. 2007, 8: e112-10.1186/1471-2199-8-112.

    Article  Google Scholar 

  30. Lerman LS, Silverstein K: Computational simulation of DNA melting and its applications to denaturing gradient gel electrophoresis. Methods Enzymol. 1987, 155: 482-501.

    Article  PubMed  CAS  Google Scholar 

  31. Huang Y, Kowalski D: WEB-THERMODYN: Sequence analysis software for profiling DNA helical stability. Nucleic Acids Res. 2003, 31: 3819-3821. 10.1093/nar/gkg562.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  32. Bi C, Benham CJ: WEBSIDD: server for predicting stress-induced duplex destabilized (SIDD) sites in superhelical DNA. Bioinformatics. 2004, 20: 1477-1479. 10.1093/bioinformatics/bth304.

    Article  PubMed  CAS  Google Scholar 

  33. Prashanth AK, Benham CJ: Susceptibility to superhelically driven DNA duplex destabilization: a highly conserved property of yeast replication origins. PLoS Comput Biol. 2005, 1: e7-10.1371/journal.pcbi.0010007.

    Article  Google Scholar 

  34. Potaman VN, Bissler JJ, Hashem VI, Oussatcheva EA, Lu L, Shlyakhtenko LS, Lyubchenko YL, Matsuura T, Ashizawa T, Leffak M: Unpaired structures in SCA10 (ATTCT)n.(AGAAT)n repeats. J Mol Biol. 2003, 326: 1095-1111. 10.1016/S0022-2836(03)00037-8.

    Article  PubMed  CAS  Google Scholar 

  35. Benham CJ, Bi C: The analysis of stress-induced duplex destabilization in long genomic DNA sequences. J Comp Biol. 2004, 11: 519-543. 10.1089/cmb.2004.11.519.

    Article  CAS  Google Scholar 

  36. Brewer BJ, Fangman WL: The localization of replication origin on ARS plasmids in Saccharomyces cerevisiae. Cell. 1987, 51: 463-471. 10.1016/0092-8674(87)90642-8.

    Article  PubMed  CAS  Google Scholar 

  37. Kiang L, Heichinger C, Watt S, Bahler J, Nurse P: Cyclin-dependent kinase inhibits reinitiation of a normal S-phase program during G2 in fission yeast. Mol Cell Biol. 2009, 29: 4025-4032. 10.1128/MCB.00185-09.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  38. Hayano M, Kanoh Y, Matsumato S, Renard-Guillet C, Shirahige K, Masai H: Rif1 is a global regulator of timing of replication origin firing in fission yeast. Genes Dev. 2012, 26: 137-150. 10.1101/gad.178491.111.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  39. Kim S-M, Huberman JA: Influence of a replication enhancer on the hierarchies of origin efficiencies within a cluster of DNA replication origins. J Mol Biol. 1999, 288: 867-882. 10.1006/jmbi.1999.2728.

    Article  PubMed  CAS  Google Scholar 

  40. Okuno Y, Satoh H, Sekiguchi M, Masukata H: Clustered adenine/thymine stretches are essential for function of a fission yeast replication origin. Mol Cell Biol. 1999, 19: 6699-6709.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  41. Chuang RY, Kelly TJ: The fission yeast homologue of Orc4 binds to replication origin DNA via multiple AT- hooks. Proc Natl Acad Sci U S A. 1999, 96: 2656-2661. 10.1073/pnas.96.6.2656.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  42. Lee JK, Moon KY, Jiang Y, Hurwitz H: The Schizosaccharomyces pombe origin recognition complex interacts with multiple AT-rich regions of the replication origin DNA by means of the AT-hook domains of the spOrc4 protein. Proc Natl Acad Sci U S A. 2001, 98: 13589-13594. 10.1073/pnas.251530398.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  43. Xu J, Yanagisawa Y, Tsankov AM, Hart C, Aoki K, Kommajosyula N, Steinmann KE, Bochicchio J, Russ C, Regev A: Genome-wide identification and characterization of replication origins by deep sequencing. Genome Biol. 2012, 13: R27-10.1186/gb-2012-13-4-r27.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  44. Givens RM, Lai WKM, Rizzo JM, Bard JE, Mieczkowski PA, Leatherwood JM, Huberman JA, Buck MJ: Chromatin architectures at fission yeast transcriptional promoters and replication origins. Nucleic Acids Res. 2012, 40: 7176-7189. 10.1093/nar/gks351.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  45. Liachko I, Bhaskar A, Lee C, Chung SC, Tye BK, Keich U: A comprehensive genome-wide map of autonomously replicating sequences in a naive genome. PLoS Genet. 2010, 6: e1000946-10.1371/journal.pgen.1000946.

    Article  PubMed  PubMed Central  Google Scholar 

  46. Costas C, Sanchez Mdela P, Stroud H, Yu Y, Oliveros JC, Feng S, Benguria A, Vidriero LI, Zhang X, Solano R: Genome-wide mapping of Arabidopsis thaliana origins of DNA replication and their associated epigenetic marks. Nat Struct Mol Biol. 2011, 18: 395-401. 10.1038/nsmb.1988.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  47. Koren A, Tsai HJ, Tirosh I, Burrack LS, Barkai N, Berman J: Epigenetically-inherited centromere and neocentromere DNA replicates earliest in S-phase. PLoS Genet. 2010, 6: e1001068-10.1371/journal.pgen.1001068.

    Article  PubMed  PubMed Central  Google Scholar 

  48. Giacca M, Zentilin L, Norio P, Diviacco S, Dimitrova D, Contreas G, Biamonti G, Perini G, Weighardt F, Riva S: Fine mapping of a replication origin of human DNA. Proc Natl Acad Sci U S A. 1994, 91: 7119-7123. 10.1073/pnas.91.15.7119.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  49. Dijkwel PA, Hamlin JL: The Chinese Hamster dihydrofolate reductase origin consists of multiple potential nascent-strand start sites. Mol Cell Biol. 1995, 15: 3023-3031.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  50. Francino MP, Ochman H: Strands symmetry around the β-globin origin of replication in primates. Mol Biol Evol. 2000, 17: 416-422. 10.1093/oxfordjournals.molbev.a026321.

    Article  PubMed  CAS  Google Scholar 

Download references


The authors are thankful to Joel A. Huberman, Rajiva Raman, Nicholas R. Rhind and Kaustuv Sanyal for critical reading of the manuscript. This work was facilitated by the internet facility provided to VBSPU by UPDESCO.

This work was supported by Council of Scientific and Industrial Research [grant #38(1233)/09/EMRII] to DDD. The award of direct Senior Research Fellowships to MPY, SP from Council of Scientific and Industrial Research and to RKM from Indian Council of Medical Research is gratefully acknowledged.

Author information

Authors and Affiliations


Corresponding author

Correspondence to Dharani D Dubey.

Additional information

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

MPY and SP analyzed chromosome 1. MPY did the deletion analysis of the ura4 origin region and weak origins. SP did the 2D origins and the origins in other organisms, compiled the figures and tables. VPT analyzed chromosome 2 and RKM analyzed chromosome 3. DDD conceptualized the analysis, analyzed the raw data and wrote the manuscript. All authors read the final version of the manuscript and agreed upon the findings reported therein.

Mukesh P Yadav, Sreedevi Padmanabhan contributed equally to this work.

Electronic supplementary material


Additional file 1:Table S1. The size, AT% and lowest G(x) values of all the intergenes proven as origins by 2D technique in 1-kb upstream and downstream, 5-Kb and 10-Kb windows centered on origins (OIRs). (PDF 38 KB)


Additional file 2:Table S2. Table showing lowest G(x) values, size, AT% of the ChIP-chip origins and AT-islands, weak origins, genes and NOIRs analyzed in 5-kb context. (XLSX 117 KB)

Authors’ original submitted files for images

Rights and permissions

Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and permissions

About this article

Cite this article

Yadav, M.P., Padmanabhan, S., Tripathi, V.P. et al. Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe. BMC Res Notes 5, 643 (2012).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: