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  1. Lipofection-mediated introduction of the CRISPR/Cas9 system in porcine zygotes provides a simple method for gene editing, without requiring micromanipulation. However, the gene editing efficiency is inadequate...

    Authors: Qingyi Lin, Quynh Anh Le, Koki Takebayashi, Chommanart Thongkittidilok, Manita Wittayarat, Maki Hirata, Fuminori Tanihara and Takeshige Otoi

    Citation: BMC Research Notes 2021 14:389

    Content type: Research note

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  2. Due to different concerns in using appropriate mosquito blood feeding techniques, this work focused on evaluating the effectiveness of two artificial mosquito feeding systems (Rutledge and Hemotek) and three d...

    Authors: Fatoumata Seck, Aurélie Cailleau, Mawlouth Diallo and Ibrahima Dia

    Citation: BMC Research Notes 2021 14:388

    Content type: Research note

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  3. The mangrove cricket, Apteronemobius asahinai, shows endogenous activity rhythms that synchronize with the tidal cycle (i.e., a free-running rhythm with a period of ~ 12.4 h [the circatidal rhythm]). Little is kn...

    Authors: Aya Satoh, Miwako Takasu, Kentaro Yano and Yohey Terai

    Citation: BMC Research Notes 2021 14:387

    Content type: Data note

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  4. To identify sociodemographic factors and health conditions associated with self-rated wellbeing for Aboriginal and Torres Strait Islander adults. Participants were recruited via investigator networks and an on...

    Authors: A. Gall, A. Diaz, G. Garvey, K. Anderson, D. Lindsay and K. Howard

    Citation: BMC Research Notes 2021 14:386

    Content type: Research note

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  5. The objective of this study was to employ ensemble clustering and tree-based risk model approaches to identify interactions between clinicogenomic features for colorectal cancer using the 100,000 Genomes Project.

    Authors: Yuguo Wei, Nikolaos Papachristou, Stefanie Mueller, Wai Hoong Chang and Alvina G. Lai

    Citation: BMC Research Notes 2021 14:385

    Content type: Research note

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  6. We aimed to detect the effect of a couple of parameters including Alg, H2O2, and HRP concentrations on the gelation time of Alg-based hydrogels using an enzymatic cross-linked procedure.

    Authors: Sepideh Saghati, Ali Baradar Khoshfetrat, Hamid Tayefi Nasrabadi, Leila Roshangar and Reza Rahbarghazi

    Citation: BMC Research Notes 2021 14:384

    Content type: Research note

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  7. The mammalian Notch ligand DLL1 has essential functions during development. To visualise DLL1 in tissues, for sorting and enrichment of DLL1-expressing cells, and to efficiently purify DLL1 protein complexes w...

    Authors: Karin Schuster-Gossler, Karsten Boldt, Dorothee Bornhorst, Patricia Delany-Heiken, Marius Ueffing and Achim Gossler

    Citation: BMC Research Notes 2021 14:383

    Content type: Research note

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  9. Bacteriophages (or phages) are viruses which infect and lyse bacteria. The therapeutic use of phages (phage therapy) has regained attention in the last decades as an alternative strategy to treat infections ca...

    Authors: Luca G. Valente, Lea Federer, Manuela Iten, Denis Grandgirard, Stephen L. Leib, Stephan M. Jakob, Matthias Haenggi, David R. Cameron, Yok-Ai Que and Josef Prazak

    Citation: BMC Research Notes 2021 14:381

    Content type: Research note

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  10. We aimed to simplify our fetal RHD genotyping protocol by changing the method to attach Illumina’s sequencing adaptors to PCR products from the ligation-based method to a PCR-based method, and to improve its reli...

    Authors: Asuka Hori, Hiroko Ogata-Kawata, Aiko Sasaki, Ken Takahashi, Kosuke Taniguchi, Ohsuke Migita, Akihiro Kawashima, Aikou Okamoto, Akihiko Sekizawa, Haruhiko Sago, Fumio Takada, Kazuhiko Nakabayashi and Kenichiro Hata

    Citation: BMC Research Notes 2021 14:380

    Content type: Research note

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  12. This study aimed to evaluate the effect of swimming training (T) on the renal system and body composition parameters in young animals treated with a high sucrose diet (SUD) during 12 weeks.

    Authors: Jaqueline A. de Souza, Angélica B. Gonçalves Pinto, Emerson C. de Oliveira, Daniel B. Coelho, Nádia L. Totou, Wanderson G. de Lima and Lenice K. Becker

    Citation: BMC Research Notes 2021 14:378

    Content type: Research note

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  13. The study of care trajectories of psychiatric patients across hospitals was previously not possible in Belgium as each hospital stores its data autonomously, and government-related registrations do not contain...

    Authors: Eva Rens, Joris Michielsen, Geert Dom, Roy Remmen and Kris Van den Broeck

    Citation: BMC Research Notes 2021 14:377

    Content type: Research note

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  14. The characterization of microRNAs (miRNA) in recent years is an important advance in the field of gene regulation. To this end, several approaches for miRNA expression analysis and various bioinformatics tools...

    Authors: Maria Tsagiopoulou, Anastasis Togkousidis, Nikolaos Pechlivanis, Maria Christina Maniou, Aristea Batsali, Angelos Matheakakis, Charalampos Pontikoglou and Fotis Psomopoulos

    Citation: BMC Research Notes 2021 14:376

    Content type: Research note

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  15. Evidence on socioeconomic inequalities in infections with the novel coronavirus (SARS-CoV-2) is still limited as most of the available studies are ecological in nature and individual-level data is sparse. We t...

    Authors: Benjamin Wachtler, Stephan Müters, Niels Michalski, Carmen Koschollek, Stefan Albrecht, Sebastian Haller, Osamah Hamouda, Claudia Hövener and Jens Hoebel

    Citation: BMC Research Notes 2021 14:375

    Content type: Research note

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  16. COVID-19 has been introduced by the World Health Organization as a health emergency worldwide. Up to 9% of the patients with COVID-19 may be readmitted by 2 months after discharge. This study aimed to estimate...

    Authors: Mohammad Nematshahi, Davood Soroosh, Mahboubeh Neamatshahi, Fahimeh Attarian and Faeze Rahimi

    Citation: BMC Research Notes 2021 14:374

    Content type: Research note

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  17. Increasing access to quality, evidence-based mental health treatments, including psychotherapy, is a global priority. Knowledge of factors associated with delivery settings is critical to ensure that new pract...

    Authors: Kimberly Hook, Julia Kozishkurt, Olga Kovalchuk, Evelina Goncharenko, Vitalii Kodovbetskyi, Milana Opanasenko, Andrii Kopytko, Andriy Girnyk, Kateryna Kliuzko, Mari-Lynn Drainoni and Sergiy Bogdanov

    Citation: BMC Research Notes 2021 14:373

    Content type: Research note

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  20. Building on findings reported in a previous publication, the objective of this study is to explore if teacher-rated indicators of school ethos modify the association between problematic familial alcohol use an...

    Authors: Gabriella Olsson, Sara Brolin Låftman, Joakim Wahlström and Bitte Modin

    Citation: BMC Research Notes 2021 14:370

    Content type: Research note

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  21. Papillary Thyroid carcinoma accounts for more than 60% of adult thyroid carcinomas. Finding a helpful marker is vital to determine the correct treatment approach. The present study was aimed to evaluate the ex...

    Authors: Mohadeseh Hajian, Abolghasem Esmaeili and Ardeshir Talebi

    Citation: BMC Research Notes 2021 14:369

    Content type: Research note

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  24. Among the different methods to profile the genome-wide patterns of transcription factor binding and histone modifications in cells and tissues, CUT&RUN has emerged as a more efficient approach that allows for ...

    Authors: Joseph Boyd, Princess Rodriguez, Hilde Schjerven and Seth Frietze

    Citation: BMC Research Notes 2021 14:366

    Content type: Research note

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  25. To explore the acceptability and feasibility of choice architecture strategies for dietary change in UK secondary school canteens from the perspectives of pupils, school staff and catering providers through qu...

    Authors: Marie Murphy, Daniel Mensah, Elena Mylona and Oyinlola Oyebode

    Citation: BMC Research Notes 2021 14:365

    Content type: Research note

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  26. The increased prevalence of metabolic dyslipidemia (MD) and its association with a variety of disorders raised a lot of attention to its management. Caveolin 1 (CAV1) the key protein in the caval structure of ...

    Authors: Nasim Khatibi, Atieh Mirzababaei, Farideh Shiraseb, Faezeh Abaj, Fariba Koohdani and Khadijeh Mirzaei

    Citation: BMC Research Notes 2021 14:364

    Content type: Research note

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  27. Neuroscience research using laboratory animals has increased over the years for a number of reasons. Some of these studies require the use of anesthetics for surgical procedures. However, the use of anesthetic...

    Authors: Gabriela Cruz Pereira, Marcos Mônico Neto, Hanna Karen Moreira Antunes, Kil Sun Lee and Marcio Henrique Mello da Luz

    Citation: BMC Research Notes 2021 14:363

    Content type: Research note

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  28. This study aims to examine changes in patients’ perspectives and outlooks regarding the disease and their health after hospitalization for COVID-19 and investigate their discrimination and harassment experienc...

    Authors: Ken Goda, Tsuneaki Kenzaka, Shinsuke Yahata, Ayako Kumabe, Masahiro Katsurada and Hogara Nishisaki

    Citation: BMC Research Notes 2021 14:362

    Content type: Research note

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  29. Realimentation can compensate for weight loss from poor-quality feedstuffs or drought. Mature cows fluctuate in body weight throughout the year due to nutrient availability. The objective of this study was to ...

    Authors: Hannah C. Cunningham-Hollinger, Larry A. Kuehn, Kristi M. Cammack, Kristin E. Hales, William T. Oliver, Matthew S. Crouse, Celine Chen, Harvey C. Freetly and Amanda K. Lindholm-Perry

    Citation: BMC Research Notes 2021 14:361

    Content type: Research note

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  30. Children with functional abdominal pain disorders (FAPD) and clinical elevations in three risk areas (anxiety, functional disability, and pain) have been found to be at increased risk for persistent disability...

    Authors: Erin L. Moorman, Michael Farrell, Neha Santucci, Lee Denson, Christine Le and Natoshia R. Cunningham

    Citation: BMC Research Notes 2021 14:360

    Content type: Research note

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  31. Extracellular matrix proteins play important roles in embryonic development and antibodies that specifically detect these proteins are essential to understanding their function. The zebrafish embryo is a popul...

    Authors: Jonathon C. Reeck, Makenna J. Hardy, Xinzhu Pu, Cynthia Keller-Peck and Julia Thom Oxford

    Citation: BMC Research Notes 2021 14:359

    Content type: Research note

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  34. While there is evidence on the short-term effects of unilateral balance training (BT) on bipedal balance performance, less is known on the acute effects of unilateral BT on unilateral (i.e., ipsi- and contrala...

    Authors: Thomas Muehlbauer, Leander Abel, Simon Schedler and Stefan Panzer

    Citation: BMC Research Notes 2021 14:356

    Content type: Research note

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  35. Induction of labour (IOL), or starting labour artificially, can be a lifesaving intervention for pregnant women and their babies, and rates are rising significantly globally. As rates increase, it becomes incr...

    Authors: Shuchita Mundle, Hillary Bracken, Vaishali Khedikar, Jayashree Mulik, Brian Faragher, Thomas Easterling, Simon Leigh, Paul Granby, Alan Haycox, Mark A. Turner, Kate Lightly, Miroslava Ebringer, Zarko Alfirevic, Beverly Winikoff and Andrew D. Weeks

    Citation: BMC Research Notes 2021 14:355

    Content type: Data note

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  36. Diffuse Large B Cell Lymphoma (DLBCL) is the most common type of Non-Hodgkin Lymphoma (NHL). The aim of this study was to assess the clinico pathological characteristics of DLBCL specifically, among the affect...

    Authors: H. Mahmood, M. Habib, W. Aslam, S. Khursheed, S. Fatima, S. Aziz, M. Habib and M. Faheem

    Citation: BMC Research Notes 2021 14:354

    Content type: Research note

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  38. Illumina BeadChip arrays are commonly used to generate DNA methylation data for large epidemiological studies. Updates in technology over time create challenges for data harmonization within and between studie...

    Authors: Lauren A. Vanderlinden, Randi K. Johnson, Patrick M. Carry, Fran Dong, Dawn L. DeMeo, Ivana V. Yang, Jill M. Norris and Katerina Kechris

    Citation: BMC Research Notes 2021 14:352

    Content type: Research note

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  39. As in most organisms, the surface of the fruit fly Drosophila melanogaster is associated with bacteria. To examine whether this association depends on cuticle quality, we isolated and quantified surface bacteria ...

    Authors: Vladislav Mokeev, Justin Flaven-Pouchon, Yiwen Wang, Nicole Gehring and Bernard Moussian

    Citation: BMC Research Notes 2021 14:351

    Content type: Research note

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  40. The public health message ‘move for health’ is relevant given the high prevalence of insufficient physical activity, particularly in African countries. The call for behaviour modification including limiting un...

    Authors: Philippe Jean-Luc Gradidge, Catherine E. Draper, Daleen Casteleijn and António Palmeira

    Citation: BMC Research Notes 2021 14:350

    Content type: Research note

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  41. This study aimed to explore current attitudes towards sun protection, and sun-seeking behaviour among young Australian adolescents. It was done as part of a larger project aiming to develop a digital resource ...

    Authors: Nisali Gamage, Rebecca Nguyen, Isabelle M. Clare, Robyn M. Lucas, Mark Strickland, Joanna Granich and Shelley Gorman

    Citation: BMC Research Notes 2021 14:349

    Content type: Research note

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  42. Papaver decaisnei Hochst. & Steud. Ex Elkan and Papaver glaucum Boiss. & Hausskn. growing wild in Northern Iraq have been historically used for medicinal purposes. In this study, both species were evaluated for t...

    Authors: Hawraz Jawdat Jafaar, Ovgu Isbilen, Ender Volkan and Gunay Sariyar

    Citation: BMC Research Notes 2021 14:348

    Content type: Research note

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  43. Cenotes are flooded caves in Mexico’s Yucatan peninsula. Many cenotes are interconnected in an underground network of pools and streams forming a vast belowground aquifer across most of the peninsula. Many pla...

    Authors: Alejandra Escobar-Zepeda, Patricia Rosas-Escobar, Laura Marquez Valdelamar, Patricia de la Torre, Laila P. Partida-Martinez, Ruben Remegaldo, Alejandro Sanchez-Flores and Fredd Vergara

    Citation: BMC Research Notes 2021 14:333

    Content type: Research note

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  44. Existing information on Arctic marine food web structure is fragmented. Integrating data across research programs is an important strategy for building a baseline understanding of food web structure and functi...

    Authors: Ashley Ehrman, Carie Hoover, Carolina Giraldo, Shannon A. MacPhee, Jasmine Brewster, Christine Michel, James D. Reist, Michael Power, Heidi Swanson, Andrea Niemi, Wojciech Walkusz and Lisa Loseto

    Citation: BMC Research Notes 2021 14:347

    Content type: Data note

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  45. Studies on the genetic background of patients with multiple myeloma (MM) have been increasing; two important factors considered in such works are uncoupling protein-2 (UCP-2) and nuclear receptor subfamily 3 g...

    Authors: Ilknur Demir, Sacide Pehlivan, Vahap Okan, Handan Haydaroglu Sahin, Salih Sertaç Durusoy, Istemi Serin, Yasemin Oyaci and Mustafa Pehlivan

    Citation: BMC Research Notes 2021 14:346

    Content type: Research note

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  46. Species identification of Shigella isolates are so prominent for epidemiological studies and infection prevention strategies. We developed and evaluated RAPD and ERIC-PCR coupled with HRM for differentiation of n...

    Authors: Babak Pakbin, Afshin Akhondzadeh Basti, Ali Khanjari, Leila Azimi, Wolfram Manuel Brück and Abdollah Karimi

    Citation: BMC Research Notes 2021 14:345

    Content type: Research note

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  47. To assess intimate partner violence screening for males and females in a health system that underwent a systemic intervention to improve survivor identification and response. Electronic health record data from...

    Authors: Cari Jo Clark, Lynette M. Renner, Qi Wang, Nyla I. Flowers, Grace Morrow and Mary Logeais

    Citation: BMC Research Notes 2021 14:344

    Content type: Research note

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  48. Bullying victimisation (BV) among children in South Africa has been identified as a major public health concern. While several studies report on the prevalence rates of BV, there is currently a dearth of resea...

    Authors: Donnay Manuel, Sabirah Adams, Mulalo Mpilo and Shazly Savahl

    Citation: BMC Research Notes 2021 14:342

    Content type: Research note

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  49. The incidence of ductal carcinoma in situ (DCIS) is increasing due to more widespread mammographic screening. DCIS, the earliest form of breast cancer, is non-invasive at the time of detection. If DCIS tissues...

    Authors: Tomoo Jikuzono, Eriko Manabe, Shoko Kure, Haruki Akasu, Tomoko Ishikawa, Yoko Fujiwara, Masujiro Makita and Osamu Ishibashi

    Citation: BMC Research Notes 2021 14:340

    Content type: Data note

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  50. Our aim was to analyse the chromogranin A-derived peptides vasostatin and catestatin in serum from wild boar (Sus scrofa) captured in a corral trap. Acute capture-related stress quickly leads to a release of adre...

    Authors: Åsa Fahlman, Johan Lindsjö, Ulrika A. Bergvall, Erik O. Ågren, Therese Arvén Norling, Mats Stridsberg, Petter Kjellander and Odd Höglund

    Citation: BMC Research Notes 2021 14:337

    Content type: Research note

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